<i>At</i>L1 a Non-LTR Retrotrasposon Fragment in the Genome of <i>Arabidopsis thaliana </i> with Homology to Plants and Animals — Oak Academic Publishing
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<i>At</i>L1 a Non-LTR Retrotrasposon Fragment in the Genome of <i>Arabidopsis thaliana </i> with Homology to Plants and Animals
Department of Life Sciences, University of Parma, Parma, Italy
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Department of Life Sciences, University of Parma, Parma, Italy
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Department of Physics and Earth Sciences “Macedonio Melloni”, University of Parma, Parma, Italy
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Department of Life Sciences, University of Parma, Parma, Italy
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Department of Life Sciences, University of Parma, Parma, Italy
1 Department of Life Sciences, University of Parma, Parma, Italy
2 Department of Life Sciences, University of Parma, Parma, Italy
3 Department of Physics and Earth Sciences “Macedonio Melloni”, University of Parma, Parma, Italy
4 Department of Life Sciences, University of Parma, Parma, Italy
5 Department of Life Sciences, University of Parma, Parma, Italy
We report the isolation of At L1, a 249 bp non-LTR retrotransposon fragment from Arabidopsis thaliana by fingerprint ing mRNAs extracted from A. thaliana plants, ecotype Columbia, in different heat stress conditions. Southern blot and PCR analysis suggested that At L1 occurs as a single- or low-copy insert in the genome of A. thaliana ecotype Columbia. The presence of At L 1 in the genome of different Arabidopsis ecotypes was confirmed by PCR amplification and se quencing thus excluding all possible contamination. A preliminary scan of the At L1 nucleotide sequence against the EMBL and NCBI databases revealed a high degree of similarity to a group of LINE type L1 retrotransposons of mam mals and with a cDNA sequence of Artemisia annua . A phylogenetic analysis of LINE elements from animals and plants placed At L1 and A. annua sequences in close proximity to some mammalian sequences but distant from the other plants LINE elements including those from Arabidopsis .
KeywordsArabidopsisHeat ShockPlant RetrotransposonsLINE RetrotransposonsHorizontal Transposon Transfer (HTT)
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