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Response of Explants of Calli Rice (<i>Oryza sativa</i> L.) Japonica cv. “Ilmi” to Gene Transformation Using <i>Agrobacterium tumefaciens</i>-Mediated
Agrotechnology Study Program, Faculty of Agriculture, Jember University, Jember, Indonesia
Division of Plant Biosciences, School of Applied Biosciences, College of Agriculture & Life Science, Kyungpook National University, Daegu, South Korea
Agrotechnology Study Program, Faculty of Agriculture, Jember University, Jember, Indonesia
Division of Plant Biosciences, School of Applied Biosciences, College of Agriculture & Life Science, Kyungpook National University, Daegu, South Korea
- 1 Agrotechnology Study Program, Faculty of Agriculture, Jember University, Jember, Indonesia
- 2 Division of Plant Biosciences, School of Applied Biosciences, College of Agriculture & Life Science, Kyungpook National University, Daegu, South Korea
- 3 Agrotechnology Study Program, Faculty of Agriculture, Jember University, Jember, Indonesia
- 4 Division of Plant Biosciences, School of Applied Biosciences, College of Agriculture & Life Science, Kyungpook National University, Daegu, South Korea
American Journal of Plant Sciences·Volume 04 (2013)·Pages 838–843·Published 11 April 2013·DOI10.4236/ajps.2013.44102
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Abstract
The AtBI-1 gene encoding the Arabidopsis thaliana Bax inhibitor was introduced into Japonica cultivars of rice ( “ Ilmi ” ) by Agrobacterium-mediated transformation, and a large number of transgenic plants were produced. The neomycin phosphotransferase II (NPTII) gene was used as a selectable marker. The activity of neomycin phosphotransferase could be successfully detected in transgenic rice calluses. Introduction of the AtBI-1 gene was also confirmed by PCR using AtBI-1 specific oligonucleotide primers in regenerated plants. Stable integration and expression of the AtbI-1 gene in plants were confirmed by GFP analysis .
KeywordsAtBI-1Agrobacterium-MediatedGFPTransformationRice
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