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Improved Micropropagation in <i>Hybanthus enneaspermus</i> L. Muell

¹ Department of Botany, Virudhunagar Hindu Nadar Senthikumara Nadar College (Autonomous), Virudhunagar, India;
² Department of Biotechnology, Pondicherry University, Pondicherry, India;
³ Department of Botany, Virudhunagar Hindu Nadar Senthikumara Nadar College (Autonomous), Virudhunagar, India
⁴ Department of Biotechnology, Government College Technology, Coimbatore, India.
⁵ Department of Botany, Virudhunagar Hindu Nadar Senthikumara Nadar College (Autonomous), Virudhunagar, India;

American Journal of Plant Sciences·Volume 04 (2013)·Pages 1169–1172·Published 4 June 2013·DOI10.4236/ajps.2013.46144

Abstract

Multiple shoots were induced on the cut ends of the leaf of Hybanthus enneaspermus on MS medium fortified with different concentration of cytokinins (KI and BAP) and in combination of KI and BAP. To enhance the multiple shoots induction , different type s of auxins were used. IAA induced a maximum of 52.3 shoots per explants in combination with KI + BAP. The regenerated shoots show two different morphological leaves. Shoots are 100% rooted on half - strength MS medium supplemented with 2.46 μM · l - 1 IBA. Rooted plantlets were successfully transferred to garden after acclimatization and they appeared morphologically normal.

Keywords&lti&gtHybanthus enneaspermus&lt/i&gtMultiple ShootsRegenerationMorphology

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Improved Micropropagation in <i>Hybanthus enneaspermus</i> L. Muell

Abstract

References (15)