Proteomics Uncovers a Role for Enhanced Ultraviolet-B Radiation on Wheat Leaves

Abstract

Wheat ( Triticum aestivum ) , as a kind of important economic crop cultured in the Northern China, is affected by pre sent-day enhanced ultraviolet-B (UV-B) radiation. To get the information of the impact by UV-B radiation on it, the protein s of wheat ( Jin mai NO .8 ) leaves, which were divided into the normal light group (CK) and UV-B radiation group (B), were extracted and ran at SDS-PAGE at different treatment days (5, 6, 7). The proteins were also analyzed by run two-dimensional gel electrophoresis (2-DE), which allowed the identification of some significantly different gel spots. The proteins spots were further verified by Matrix-Assisted Laser Desorption/lonization-time of Flight Mass Spectrometry. The results showed: 1) the enhanced UV-B affects the growth of the wheat, as the visual changes appear on the sixth day ; 2) the proteins expression s between the B group and the CK group were remarkab ly differen t on the sixth day ; 3) the proteins of wheat leaves of the sixth day were further analyzed by 2-DE revealed that twenty-one protein points were identificated between the B group and the CK group. Among these twenty-one proteins, six proteins of them were up-regulated and twelve proteins of them were down-regulated, three new proteins were expressed only in the B group. Three proteins among six proteins, which were up-regulated, were further verified as RuBisCo large subunit binding protein; SOD; Calmodulin. The result indicates wheat could improve genes encoding proteins in their leaves and protect themselves, when enhanced UV-B affects the growth of the wheat.