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<i>Arabidopsis</i> Transcription Factor WRKY33 Is Involved in Drought by Directly Regulating the Expression of <i>CesA8</i>
College of Life Science, Capital Normal University, Beijing, China.
College of Life Science, Capital Normal University, Beijing, China.
College of Life Science, Capital Normal University, Beijing, China.
College of Life Science, Capital Normal University, Beijing, China.
College of Life Science, Capital Normal University, Beijing, China.
- 1 College of Life Science, Capital Normal University, Beijing, China.
- 2 College of Life Science, Capital Normal University, Beijing, China.
- 3 College of Life Science, Capital Normal University, Beijing, China.
- 4 College of Life Science, Capital Normal University, Beijing, China.
- 5 College of Life Science, Capital Normal University, Beijing, China.
American Journal of Plant Sciences·Volume 04 (2013)·Pages 21–27·Published 19 June 2013·DOI10.4236/ajps.2013.46A004
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Abstract
Arabidopsis ( Arabidopsis thaliana ) WRKY33 is a key transcription factor in pathogen-induced defense signaling, but its function in abiotic stresses remains largely unclear. In this study, we report on the use of a reverse-genetic approach, as well as a yeast ( Saccharomyces cerevisiae ) expression system, to determine the role of WRKY33 in drought. A T-DNA insertion deletion mutant of WRKY 33 is more sensitive to dehydration. Through genome-wide screening the target genes of WRKY33 in yeast, we identified 23 candidate genes including a drought tolerance gene CesA 8 . Further results revealed that WRKY33 repressed CesA 8 expression through binding to the W-box elements of CesA 8 distal promoter region and probably interacting with the transcriptional activator of CesA 8, MYB46. These findings revealed the primary molecular mechanism underlying the function of WRKY33 in response to drought
KeywordsWRKY33Drought<i>CesA8</i>Target GeneInverse Yeast One-Hybrid Assay
- T. Eulgem, P. J. Rushton, S. Robatzek and I. E. Somssich, “The WRKY Super Family of Plant Transcription Factors,” Trends in Plant Science, Vol. 5, No. 5, 2000, pp. 199-206. doi:10.1016/S1360-1385(00)01600-9
- P. J. Rushton, I. E. Somssich, P. Ringler and Q. J. Shen, “WRKY Transcription Factors,” Trends in Plant Science, Vol. 15, No. 5, 2010, pp. 247-258. doi:10.1016/j.tplants.2010.02.006
- B. ülker and I. E. Somssich, “WRKY Transcription Factors: From DNA Binding towards Biological Function,” Current Opinion in Plant Biology, Vol. 7, No. 5, 2004, pp. 491-498. doi:10.1016/j.pbi.2004.07.012
- Z. Zheng, S. A. Qamar, Z. Chen and T. Mengiste, “Arabidopsis WRKY33 Transcription Factor Is Required for Resistance to Necrotrophic Fungal Pathogens,” The Plant Journal, Vol. 48, No. 4, 2006, pp. 592-605. doi:10.1111/j.1365-313X.2006.02901.x
- B. Lippok, R. P. Birkenbihl, G. Rivory, J. Brümmer, E. Schmelzer, E. Logemann and I. E. Somssich, “Expression of AtWRKY33 Encoding a Pathogenor PAMP-Responsive WRKY Transcription Factor Is Regulated by a Composite DNA Motif Containing W Box Elements,” Molecular Plant-Microbe Interaction, Vol. 20, No. 4, 2007, pp. 420-429. doi:10.1094/MPMI-20-4-0420
- J. L. Qiu, B. K. Fiil, K. Petersen, H. B. Nielsen, C. J. Botanga, S. horgrimsen, K. Palma, M. C. Suarez-Rodriguez, S. Sandbech-Clausen, J. Lichota, P. Brodersen, K. D. Grasser, O. Mattsson, J. Glazebrook, J. Mundy and M. Petersen, “Arabidopsis MAP Kinase 4 Regulates Gene Expression through Transcription Factor Release in the Nucleus,” EMBO Journal, Vol. 27, No. 16, 2008, pp. 2214-2221. doi:10.1038/emboj.2008.147
- G. Mao, X. Meng, Y. Liu, Z. Zheng, Z. Chen and S. Zhang, “Phosphorylation of a WRKY Transcription Factor by Two Pathogen Responsive MAPKs Drives Phytoalex in Biosynthesis in Arabidopsis,” The Plant Cell, Vol. 23, No. 4, 2011, pp. 1639-1653. doi:10.1105/tpc.111.084996
- R. P. Birkenbihl, C. Diezel and I. E. Somssich, “Arabidopsis WRKY33 Is A Key Transcriptional Regulator of Hormonal and Metabolic Responses toward Botrytis Cinerea Infection,” Plant Physiology, Vol. 159, No. 1, 2012, pp. 266-285. doi:10.1104/pp.111.192641
- Y. Jiang and M. K. Deyholos, “Comprehensive Transcriptional Profiling of NaCl-Stressed Arabidopsis Roots Reveals Novel Classes of Responsive Genes,” BMC Plant Biology, Vol. 6, 2006, p. 25. doi:10.1186/1471-2229-6-25
- Y. Jiang and M. K. Deyholos, “Functional Characterization of Arabidopsis NaCl-Inducible WRKY25 and WRKY33 Transcription Factors in Abiotic Stresses,” Plant Molecular Biology, Vol. 69, No. 1-2, 2009, pp. 91-105. doi:10.1007/s11103-008-9408-3