Characterization of Microsatellite Markers and Their Application to Genetic Diversity Analysis of <i>Brachypodium sylvaticum</i> var. <i>breviglume</i> from Yunnan, China
- 1 Plant Germplasm and Genomics Center, Germplasm Bank of Wild Species in Southwest China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, China
- 2 Plant Germplasm and Genomics Center, Germplasm Bank of Wild Species in Southwest China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, China;
- 3 Plant Germplasm and Genomics Center, Germplasm Bank of Wild Species in Southwest China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, China;
Abstract
Southwestern China is abundant in a large species diversity of grasses and Brachypodium sylvaticum var. breviglume is unquestionably appropriate to serve as an ideal model to investigate the adaptation, evolution and diversification of grasses in this region. In this study, a total of 37 microsatellite markers were screened and genetic variation was estimated for 100 individuals from the five natural populations of the species. Our results showed that seven of them were polymorphic for the five studied populations, while the other thirty were monomorphic. These seven SSR loci exhibited a high level of genetic diversity among populations, i.e. allele number varied from 5 to 24, with an average of 13.29; expected heterozygosity ( He ) ranged from 0.439 to 0.561, with an average of 0.488; percentage of polymorphic loci ( PPL ) ranged from 85.71% to 100%, with an average of 97. 14%. The FCA and UPGMA analyses revealed that the five populations were divided into three clusters. Our results indicate that these newly characterized SSR markers are useful for the exploration of genetic diversity and evolutionary history of the B. sylvaticum var. breviglume populations in Yunnan , China .
- P. Vain, “Brachypodium as a Model System for Grass Research,” Journal of Cereal Science, Vol. 54, No. 1, 2011, pp. 1-7. doi:10.1016/j.jcs.2011.04.002
- A. P. Ramakrishnan, T. Musial and M. B. Cruzan, “Shifting Dispersal Modes at an Expanding Species’ Range Margin,” Molecular Ecology, Vol. 19, No. 6, 2010, pp. 1134-1146. doi:10.1111/j.1365-294X.2010.04543.x
- D. M. Rosenthal, A. P. Ramakrishnan and M. B. Cruzan, “Evidence for Multiple Sources of Invasion and Intraspecific Hybridization in Brachypodium sylvaticum (Hudson) Beauv. in North America,” Molecular Ecology , Vol. 17, No. 21, 2008, pp. 4657-4669. doi:10.1111/j.1365-294X.2008.03844.x.
- F. Schläpfer and M. Fischer, “An Isozyme Study of Clone Diversity and Relative Importance of Sexual and Vegetative Recruitment in the Grass Brachypodium pinnatum,” Ecography, Vol. 21, No. 4, 1998, pp. 351-360. doi:10.1111/j.1600-0587.1998.tb00400.x
- P. Catalan, Y. Shi, L. Armstrong, J. Draper and C. A. Stace, “Molecular Phylogeny of the Grass Genus Brachypodium P. Beauv. Based on RFLP and RAPD Analysis,” Botanical Journal of the Linnean Society, Vol. 117, No. 4, 1995, pp. 263-280. doi:10.1111/j.1095-8339.1995.tb02590.x
- A. M. Jaroszewicz, R. Kosina and P. R. Stankiewicz “RAPD, Karyology and Selected Morphological Variation in a Model Grass, Brachypodium distachyon,” Weed Research, Vol. 52, No. 3, 2012, pp. 204-216. doi:10.1111/j.1365-3180.2012.00916.x
- E. Filiz, B. S. Ozdemir, F. Budak, J. P. Vogel, M. Tuna, and H. Budak, “Molecular, Morphological, and Cytological Analysis of Diverse Brachypodium distachyon Inbred Lines,” Genome, Vol. 52, No. 10, 2009, pp. 876-890. doi:10.1139/g09-062
- P. Martínez-Gómez, R. Sánchez-Pérez, F. Dicenta, W. Howad, P. Arus and T. M. Gradziel, “Almonds,” In: C. R. Kole, Ed., Genome Mapping and Molecular Breeding, Fruits & Nuts, Springer, Heidelberg, Berlin, New York, Tokyo, Vol. 4, 2007, pp. 229-242.
- P. K. Gupta, H. S. Balyan, P. C. Sharma and B. Ramesh, “Microsatellites in Plants: A New Class of Molecular Markers,” Current Science, Vol. 70, 1996, pp. 45-54.
- Y. Keng, “Brachypodium sylvaticum var. breviglume Keng ex Keng f.,” Acta Botanica Yunnanica, Vol. 4, 1982, pp. 277-278.
- M. A. Kahn and C. A. Stace, “Breeding Relationships in the Genus Brachypodium (Poaceae: Pooideae),” Nordic Journal of Botany, Vol. 19, No. 3, 1999, pp. 257-269. doi:10.1111/j.1756-1051.1999.tb01108.x