Nodulation in Onobrychis Perennial Legume Plants
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Abstract
A total of 110 strains of nodule bacteria was isolated from plants Onobrychis transcaucasica and Onobrychis chorassanica. Nodulation study of bacteria in both Onobrychis plant species in microvegetation experiment gave a very low nodulation on plant roots. The intensive nodulation of Onobrychis plants was recorded in vegetation experiment and for Onobrychis transcaucasica the efficiently-nodulating strains were found OT102, OT103, OT117, OT121, OT130, OT136, ОT139, ОT140, while for Onobrychis chorassanica plants—ОC106, ОC107, ОC109, ОC112, ОT103, ОT117 and ОT123 strains. Nucleotide sequencing of the 16S rRNA gene and BLAST analysis showed that nodule bacteria of Onobrychis plants were related to Rhizobium, Burkholderia, Enterobacter and Pantoea genera. It has been shown a possibility of growing up of Onobrychis plants at minimal additional moisture of sabulous soils in the Kyzyl Kum Desert, creating artificial pastures and thereby immobilizing the desert blown sands.
- M. D. Rumbaugh, “Special Purpose Forage Legumes,” In: J. Janick and J. E. Simon, Ed., Advances in New Crops. Timber Press, Portland, OR, 1990, pp. 183-190.
- H. Blasum, “Wild legumes, vetch inoculation, and rhizobial taxonomy in Xiaman farm, eastern edge of the Qinghai-Tibet plateau”, MS Thesis. http://www.blasum.net/ holger/wri/alpleg/index.html
- D. Prévost, P. Drouin, S. Laberge, A. Bertrand, J. Cloutier and G. Lévesque, “Cold-Adapted Rhizobia for Nitrogen Fixation in Temperate Regions,” Canadian Journal of Botany, Vol. 81, 2003, pp. 1153-1161.
- G. Laguerre, P. Van Berkum, N. Amarger and D. Prevost, “Genetic Diversity of Rhizobial Sym-bionts Isolated from Legume Species within the Genera Astragalus, Oxytropis and Onobrychis,” Applied Environmental Microbiology, Vol. 63, December 1997, pp. 4748-4758.
- D. Prevost, L. M. Bordeleau and H. Antoun, “Symbiotic Effectiveness of Indigenous Arctic Rhizobia on a Temperate Forage Legume: Sainfoin (Onobrychis viciifolia),” Plant and Soil, Vol. 104, No. 1, March 1987, pp. 63-69.
- D. Prévost, H. Antoun and L. M. Bordeleau, “Effects of Low Temperature on Nitrogenase Activity in Sainfoin (Onobrychis viciifolia) Nodulated by Arctic Rhizobia,” FEMS Microbiology Ecology, Vol. 45, 1987, pp. 205-210.
- R. J. Roughley, “The Influence of Root Temperature, Rhizobium Strain and Host Selection on the Structure and Nitrogen-Fixing Efficiency of the Root Nodules of Trifolium subterraneum,” Annals of Botany, Vol. 34, 1970, pp. 631-664.
- P. H. Graham, “Co-evolution of Phaseolus Vulgaris and Its Rhizobia,” University of Minnesota, Department of Soil, Water, and Climate, College of Agricultural, Food, and Environmental Sciences, Rhizobia Research Laboratory.
- Z. Zhao, S. E. Williams and G. E. Schuman, “Renodulation and Characterization of Rhizobium Isolates from Cicer Milkvetch (Astragalus cicer L.),” Biology and Fertility of Soils, Vol. 25, No. 2, August 1997, pp. 169-174.
- A. U. Khotyanovich, “Methods for Cultivation of Nitrogen-Fixing Bacteria, Ways of Both Their Obtaining And Preparing of Preparations on Their Base (Methodical Guides),” All-Union Institute of Agricultural Microbiology, Leningrad, 1991, pp. 33-60.
- D. R. Hoagland, D. J. Arnon, “The Water Culture Method for Growing Plants without Soil,” California Agricultural Experimental Station, Vol. 347, 1938, pp. 1-32.
- D. W. Hardy, R. Halstein, E. Jakson and R. S. Buens, “C2H2–C2H4 Assay to N2 Fixation Laboratory and Field Evaluation,” Plant Physiology, Vol. 43, 1968, pp. 9-13.