Influence of Boric Acid and Sucrose on the Germination and Growth of Areca Pollen
- 1 Hainan Key Laboratory of Tropical Oil Crops Biology/Coconut Research Institute, Chinese Academy of Tropical Agricultural Sci- ences, Wenchang, China.
- 2 Hainan Key Laboratory of Tropical Oil Crops Biology/Coconut Research Institute, Chinese Academy of Tropical Agricultural Sci- ences, Wenchang, China.
- 3 Hainan Key Laboratory of Tropical Oil Crops Biology/Coconut Research Institute, Chinese Academy of Tropical Agricultural Sci- ences, Wenchang, China.
Abstract
This study investigates the effect of boric acid and sucrose on the germination and growth of Areca catechu L (areca palm). Using areca pollen from Hainan, China, this study determines the influence of culture media with different con centrations of boric acid and sucrose on the germination and growth of areca pollen. Results show that the germination and growth of the pollen tube growth are highest under a boric acid concentration of 0.4 g /L to 0.6 g /L. The germination rate of areca pollen is highest in solid medium with a sucrose concentration of 40 g /L. The optimum culture medium for developing areca pollen comprises 5 g /L agar, 0.4 g /L to 0.6 g /L boric acid, 40 g /L sucrose, and water. Furthermore, areca pollen grows most rapidly approximately 1.5 h after in vitro cultivation at normal temperature, and growth stabi lizes at approximately 3 h. The principle that governs the development of areca pollen is also discussed.
- R. C. Pan, X. J. Wang and N. H. Li, “Plant Physiology,” Higher Education Press, Beijing, 2008, p. 36.
- T. Zhang and M. Huang, “The Effect of Sucrose and PEG on the in Vitro Germination of Camellia Pollen,” Northern Horticulture, Vol. 1, 2009, pp. 101-102.
- X. P. Liu, J. X. Tu and B. Y. Chen, “Research Progress in Yellow-Seeded Rapeseed,” Chinese Journal of Oil Crop Sciences, Vol. 27, No. 2, 2005, pp. 87-91.
- Y. H. Yang, Y. S. Xia and C. W. Du, “The Difference on the Boron Demand in the Vegetative Growth of the Rape Cultivars with Different Boron Efficiencies,” Chinese Journal of Oil Crop, Vol. 27, No. 1, 2005, pp. 69-72.
- Z. L. Zhang, J. N. Li and Z. L. Tang, “The Regulation of Environmental Conditions on Rape Quality,” Chinese Agricultural Science Bulletin, Vol. 2, 2006, pp. 124-129.
- C. C. Teng and Y. C. Zhang, “The Influence of Boric Acid and Sucrose on the in Vitro Germination of Potato Pollen,” Seed, Vol. 28, No. 2, 2009, pp. 15-20.
- G. J. Liang, G. P. Huang, L. Deng, et al., “Effect of Boron, Sugar, Calcium and DA-6 on the Growth of Loquat Pollen Tube,” Journal of Zhaoqing University, Vol. 32, No. 2, 2011, pp. 50-56.
- Zhejiang Agricultural University, “Plant Nutrition and Fertilizer Science,” Vol. 10, 1992, p. 143.
- G. S. Zhang, “Effect of Boron and Sugar on the Growth of Cucurbita Maxima Pollen Tube,” Journal of Heze University, Vol. 22, No. 2, 2000, pp. 54-55.
- T. Zhang and M. Huang, “The Effect of Sucrose and PEG on the in Vitro Pollen Germination of Camellia,” Northern Horticulture, Vol. 1, 2009, pp. 101-102.
- R. G. Stenli and H. F. Linskens, “Pollen: Biology Biochemistry Management,” Springer-Verlag, Berlin, Heidelberg, New York, 1974, p. 37.
- H. B. Zhao, W. M. Fang and F. D. Chen, “Pollen Germination in Vitro and Pollen Tube Growth of Plum,” GUIHAIA, No. 3, 2007, pp. 393-396.
- H. B. Zhao, F. D. Chen and W. M. Fang, “Pollen Germination in Vitro of Chrysanthemum Cultivars with Small Inflorescences and Several Species of Dendranthema,” Journal of Nanjing Agricultural University, Vol. 28, No. 2, 2005, pp. 22-27.
- Z. N. Li, G. F. Liu, C. L. Luo, et al., “The Viability and Storage Capacity of Platanus orientalis Pollen,” Wuhan Botanical Research, Vol. 24, No. 1, 2006, pp. 54-57.
- W. X. Peng, F. L. Li and W. L. Yang, “Determination and Comparison of the Viability of Various Xanthoceras sorbifolia Pollen,” Hebei Journal of Forestry and Orchard Research, Vol. 14, No. 1, 1999, pp. 51-53.