To create asymmetric somatic hybrids, the genome of the so-called donor protoplast is fragmented prior to protoplast fusion. As a result, only a limited amount of the donor genome is transferred to the fusion product. This technique can circumvent some commonly observed problems related to symmetric fusion and offers a practical breeding tool for asexual hybridization. Genomes are typically fragmented by irradiation, microprotoplast production or application of metabolic inhibitors such as iodoacetamide. Irradiation and microprotoplast production fragment the nuclear genome, whereas iodoacetamide inactivates the cytoplasmic genome. It can therefore be used to introduce cytoplasmic male ste rility, an important practical application. For hybrid verification and genome characterization, molecular markers and cytogenetic techniques are applied. This review highlights and discusses progress made during the last decade in sper matophytes asymmetric protoplast fusion.
Johnson and R. Veilleux, “Somatic Hybridization and Application in Plant Breeding,” In: J. Janick, Ed., Plant Breeding Rev 20, John Wiley & Sons, New York, 2001, pp. 167-225.
T. Eeckhaut, K. Van Laere, J. De Riek and J. Van Huylenbroeck, “Overcoming Interspecific Barriers in Ornamental Plant Breeding,” In: J. Teixeira da Silva, Ed., Floriculture, Ornamental and Plant Biotechnology: Advances and Topical Issues, Global Science Books, London, 2006, pp. 540-551.
J. W. Grosser and F. G. Gmitter, “2004 SIVB Congress Symposium Proceedings of Thinking Outside the Cell: Applications of Somatic Hybridization and Cybridization in Crop Improvement, with Citrus as a Model,” In Vitro Cellular & Developmental Biology Plant, Vol. 41, No. 3, 2005, pp. 220-225. doi:10.1079/IVP2004634
J. Grosser and F. Gmitter, “Protoplast Fusion for Production of Tetraploids and Triploids: Applications for Scion and Rootstock Breeding in Citrus,” Plant Cell, Tissue and Organ Culture, Vol. 104, No. 3, 2011, pp. 343-357. doi:10.1007/s11240-010-9823-4
R. Thieme, U. Darsow, L. Rakosy-Tican, Z. Kang, T. Gavrilenko, O. Antonova, U. Heimbach and T. Thieme, “Use of Somatic Hybridization to Transfer Resistance to Late Blight and Potato Virus Y (PVY) into Cultivated Potato,” Plant Breeding and Seed Science, Vol. 50, No. 1, 2004, pp. 113-118.
E. Küster, “über die Verschmelzung Nachter Protoplasten,” Berichte der Deutschen Botanischen Gesellschaft, Vol. 27, No. 10, 1909, pp. 589-598.
P. S. Carlson, H. H. Smith and R. D. Dearing, “Parasexual Interspecific Plant Hybridization,” Proceedings of the National Academy of Sciences of the United States of America, Vol. 69, No. 8, 1972, pp. 2292-2294. doi:10.1073/pnas.69.8.2292
O. L. Gamborg, “Plant Tissue Culture. Biotechnology. Milestones,” In Vitro Cellular & Developmental Biology Plant, Vol. 38, No. 2, 2002, pp. 84-92. doi:10.1079/IVP2001281
M. Davey, P. Anthony, J. Power and K. Lowe, “2004 SIVB Congress Symposium Proceedings of Thinking Outside the Cell: Plant Protoplast Technology: Status and Applications,” In Vitro Cellular & Developmental Biology Plant, Vol. 41, No. 3, 2004, pp. 202-212. doi:10.1079/IVP2004633
M. Razdan, “Somatic Hybridization,” In: Introduction to Plant Tissue Culture, Science Publishers, Enfield, 2003, pp. 147-175.
J. Liu, X. Xu and X. Deng, “Intergeneric Somatic Hybridization and Its Application to Crop Genetic Improvement,” Plant Cell, Tissue and Organ Culture, Vol. 82, No. 1, 2005, pp. 19-44. doi:10.1007/s11240-004-6015-0
S. Waara and K. Glimelius, “The Potential of Somatic Hybridization in Crop Breeding,” Euphytica, Vol. 85, No. 2, 1995, pp. 217-233. doi:10.1007/BF00023951
G. Xia, “Progress of Chromosome Engineering Mediated by Asymmetric Somatic Hybridization,” Journal of Genetics and Genomics, Vol. 36, No. 9, 2009, pp. 547-556. doi:10.1016/S1673-8527(08)60146-0
M. Skarzhinskaya, M. Landgren and K. Glimelius, “Production of Intertribal Somatic Hybrids between Brassica napus L. and Lesquerella fendleri (Gray) Wats,” Theoretical and Applied Genetics, Vol. 93, No. 8, 1996, pp. 1242-1250. doi:10.1007/BF00223456
Q. Hu, L. Hansen, J. Laursen, C. Dixelius and S. Andersen, “Intergeneric Hybrids between Brassica napus and Orychophragmus violaceus Containing Traits of Agronomic Importance for Oilseed Rape Breeding,” Theoretical and Applied Genetics, Vol. 105, No. 6-7, 2002, pp 834-840. doi:10.1007/s00122-002-1017-y
H. Cui, Z. Yu, J. Deng, X. Gao, Y. Sun and G. Xia, “Introgression of Bread Wheat Chromatin into Tall Wheatgrass via Somatic Hybridization,” Planta, Vol. 229, No. 2, 2009, pp. 323-330. doi:10.1007/s00425-008-0832-z
C. Rambaud, J. Dubois and J. Vasseur, “Male-Sterile Chicory Cybrids Obtained by Intergeneric Protoplast Fusion,” Theoretical and Applied Genetics, Vol. 87, No. 3, 1993, pp. 347-352. doi:10.1007/BF01184922
C. Li, G. Xia, F. Xiang, C. Zhou and A. Cheng, “Regeneration of Asymmetric Somatic Hybrid Plants from the Fusion of Two Types of Wheat with Russian Wildrye,” Plant Cell Reports, Vol. 23, No. 7, 2004, pp. 461-467. doi:10.1007/s00299-004-0834-7
Y. Gleba, S. Hinnisdaels, V. Sidorov, V. Kaleda, A. Parokonny, N. Boryshuk, N. Cherep, I. Negrutiu and M. Jacobs, “Intergeneric Asymmetric Hybrids between Nicotiana plumbaginifolia and Atropa belladonna Obtained by Gamma-Fusion,” Theoretical and Applied Genetics, Vol. 76, No. 5, 1988, pp. 760-766. doi:10.1007/BF00303523
D. Dudits, O. Fejer, G. Hadlaczky, C. Koncz, G. Lazar and G. Horvath, “Intergeneric Gene Transfer Mediated by Plant Protoplast Fusion,” Molecular and General Genetics, Vol. 179, No.2, 1980, pp. 283-288. doi:10.1007/BF00425455
D. Dimanov and A. Atanassov, “A Study of the Possibility to Apply UV-Light in Experiments of Somatic Hybridization in Protoplast Cultures of Nicotiana alata and N. tabacum (Line Virginia 89),” Genetics and Breeding, Vol. 22, No 1, 1989, pp. 40-44.
J. Forsberg, C. Dixelius, U. Lagercrantz and K.Glimelius, “UV Dose-Dependent DNA Elimination in Asymmetric Somatic Hybrids between Brassica napus and Arabidopsis thaliana,” Plant Science, Vol. 131, No. 1, 1998, pp. 65-76. doi:10.1016/S0168-9452(97)00242-2
M. Wang, Z. Peng, S. Hong, D. Zhi and G. Xia, “Hybrid Inflorescences Derived from Gamma-Fusion of Arabidopsis thaliana with Bupleurum scorzonerifolium,” Protoplasma, Vol. 249, No. 1, 2012, pp. 197-205. doi:10.1007/s00709-011-0278-1
R. Hall, F. Krens and G. Rouwendal, “DNA RadiationDamage and Asymmetric Somatic Hybridization: Is Uv a Potential Substitute or Supplement to Ionizing-Radiation in Fusion Experiments,” Physiologia Plantarum, Vol. 85, No. 2, 1992, pp. 319-324. doi:10.1111/j.1399-3054.1992.tb04741.x
D. Deryckere, “Development of Asymmetric Somatic Hybridization Technology in Industrial Chicory (Cichorium intybus L.),” PhD Thesis, Ghent University, Ghent, 2013.
B. Navratilova, L. Luhova and M. Petrivalsky, “Effect of UV-C Irradiation on Mesophyll Protoplasts of Cucumis sativus,” Plant Cell, Tissue and Organ Culture, Vol. 94, No. 3, 2008, pp. 313-318. doi:10.1007/s11240-008-9416-7
Y. Abas, N. Touil, M. Kirsch-Volders, G. Angenon, M. Jacobs and I. D. H. Famelaer, “Evaluation of UV Damage at DNA Level in Nicotiana plumbaginifolia Protoplasts using Single Cell Gel Electrophoresis,” Plant Cell, Tissue Organ Culture, Vol. 91, No. 2, 2007, pp. 145-154. doi:10.1007/s11240-007-9257-9
X. Y. Xu, Z. Y. Hu, J. F. Li, J. H. Liu and X. X. Deng, “Asymmetric Somatic Hybridization between UV-Irradiated Citrus unshiu and C. sinensis: Regeneration and Characterization of Hybrid Shoots,” Plant Cell Reports, Vol. 26, No 8, 2007, pp. 1263-1273. doi:10.1007/s00299-007-0350-7
A. Yemets and Y. Blume, “Antimitotic Drugs for Microprotoplast-Mediated Chromosome Transfer,” In: Y. Blume, W. Baird, A. Yemets and Breviario, Eds., Plant Genomics, Cell Engineering and Breeding. The Plant Cytoskeleton: A Key Tool for Agro-Biotechnology, Springer, Dordrecht, 2009, pp. 419-434.
K. S. Ramulu, P. Dijkhuis, I. Famelaer, T. Cardi and H. A. Verhoeven, “Isolation of Sub-Diploid Microprotoplasts for Partial Genome Transfer in Plants: Enhancement of Micronucleation and Enrichment of Microprotoplasts with One or a Few Chromosomes,” Planta, Vol. 190, No. 2, 1993, pp. 190-198. doi:10.1007/BF00196611
K. Vaughn, “Anticytoskeletal Herbicides,” In: P. Nick, Ed., Plant Microtubules, Potential for Biotechnology, Springer, Berlin, 2000, pp. 193-205. doi:10.1007/978-3-662-22300-0_9
K. S. Ramulu, P. Dijkhuis, E. Rutgers, J. Blaas, W. H. J. Verbeek, H. A. Verhoeven and C. M. Colijn-Hooymans, “Microprotoplast Fusion Technique: A New Tool for GeneTransfer between Sexually-Incongruent Plant Species,” Euphytica, Vol. 85, No. 1-3, 1995, 255-268. doi:10.1007/BF00023954
Q. Zhang, J. Liu and X. Deng, “Isolation of Microprotoplasts from a Partially Synchronized Suspension Culture of Citrus unshiu,” Journal of Plant Physiology, Vol. 163, No. 11, 2006, pp. 1185-1192. doi:10.1016/j.jplph.2005.09.003
I. Famelaer, H. A. Verhoeven, P. Dijkhuis and K. S. Ramulu, “A Study of the Process of Synchronisation and Micronucleation in Beta vulgaris and the Monitoring of an Isolation Procedure for Micro-Nuclei and Micro-Protoplasts by Confocal Laser Scanning Microscopy and Flow Cytometry,” Plant Cell, Tissue and Organ Culture, Vol. 90, No. 2, 2007, pp. 169-179. doi:10.1007/s11240-007-9225-4
H. Saito and M. Nakano, “Isolation and Characterization of Gametic Microprotoplasts from Developing Microspores of Lilium longiflorum for Partial Genome Transfer in the Liliaceous Ornamentals,” Sexual Plant Reproduction, Vol. 15, No. 4, 2002, pp. 179-185. doi:10.1007/s00497-002-0153-5
P. Lakshmanan, T. Eeckhaut, J. Van Huylenbroeck and E. Van Bockstaele, “Micronucleation by Mitosis Inhibitors in Developing Microspores of Spathiphyllum wallisii Regel,” Plant Cell Reports, Vol. 32, No. 3, 2013, pp. 369-377. doi:10.1007/s00299-012-1370-5
W. Guo and J. W. Grosser, “Somatic Hybrid Vigor in Citrus: Direct Evidence from Protoplast Fusion of an Embryogenic Callus Line with a Transgenic Mesophyll Parent Expressing the GFP Gene,” Plant Science, Vol. 168, No. 6, 2005, pp. 1541-1545. doi:10.1016/j.plantsci.2005.02.029
P. C. Binsfeld, R. Wingender and H. Schnabl, “Characterization and Molecular Analysis of Transgenic Plants Obtained by Microprotoplast Fusion in Sunflower,” Theoretical and Applied Genetics, Vol. 101, No. 8, 2000, pp. 1250-1258. doi:10.1007/s001220051604
F. Saito and M. Nakano, “Partial Synchronization of Cell Division and Micronucleation in Suspension-Cultured Cells of Hemerocallis hybrida: The Effects of Hydroxyurea and Various Spindle Toxins,” Breeding Science, Vol. 51, No. 4, 2001, pp. 285-291. doi:10.1270/jsbbs.51.285
S. Varotto, E. Nenz, M. Lucchin and P. Parrini, “Production of Asymmetric Somatic Hybrid Plants between Cichorium intybus L. and Helianthus annuus L.,” Theoretical and Applied Genetics, Vol. 102, No. 6-7, 2001, pp. 950-956. doi:10.1007/s001220000485
W. Xiao, X. Huang, Q. Gong, X. Dai, J. Zhao, Y. Wei and X. Huang, “Somatic Hybrids Obtained by Asymmetric Protoplast Fusion between Musa Silk cv. Guoshanxiang (AAB) and Musa acuminata cv. Mas (AA),” Plant Cell, Tissue and Organ Culture, Vol. 97, No. 3, 2009, pp. 313-321. doi:10.1007/s11240-009-9530-1
C. De Bona, J. Gould, J. Miller, D. Stelly and E. Louzada, “Citrus Asymmetric Somatic Hybrids Produced via Fusion of Gamma-Irradiated and Iodoacetamide-Treated Protoplasts,” Pesquisa Agropecuaria Brasileira, Vol. 44, No. 5, 2009, pp. 454-462. doi:10.1590/S0100-204X2009000500004
M. Wang, G. Xia and Z. Peng, “High UV-Tolerance with Introgression Hybrid Formation of Bupleurum scorzonerifolium Willd,” Plant Science, Vol. 168, No. 3, 2005, pp. 593-600. doi:10.1016/j.plantsci.2004.09.017
J. Grosser, M. Calovic and E. Louzada E, “Protoplast Fusion Technology: Somatic Hybridization and Cybridization,” In: M. Davey and P. Anthony, Eds., Plant Cell Culture: Essential Methods, John Wiley & Sons, New York, 2010, pp. 175-198. doi:10.1002/9780470686522
J. Wang, C. Zhao, C. Liu, G. Xia and F. Xiang, “Introgression of Swertia mussotii Gene into Bupleurum scorzonerifolium via Somatic Hybridization,” BMC Plant Biology, Vol. 11. No. 71, 2011. http://www.biomedcentral.com/1471-2229/11/71
L. Jiang, Y. Cai Y, G. Xia and F. Xiang, “Introgression of the Heterologous Nuclear DNAs and Efficacious Compositions from Swertia tetraptera Maxim into Bupleurum scorzonerifolium Willd via Somatic Hybridization,” Protoplasma, Vol. 249, No. 3, 2012, pp. 737-745. doi:10.1007/s00709-011-0317-y
Y. Yu, Z. Li, P. Wang and F. Xiang, “Genetic and Biochemical Characterization of Somatic Hybrids between Bupleurum scorzonerifolium and Gentianopsis paludosa,” Protoplasma, Vol. 249, No.4, 2012, pp. 1029-1035. doi:10.1007/s00709-011-0336-8
K. Taski-Ajdukovic, D. Vasic and N. Nagl, “Regeneration of Interspecific Somatic Hybrids between Helianthus annuus L. and Helianthus maximiliani (Schrader) via Protoplast Electrofusion,” Plant Cell Reports, Vol. 25, No.7, 2006, pp. 698-704. doi:10.1007/s00299-006-0134-5
X. Du, X. Ge, X. Yao, Z. Zhao and Z. Li, “Production and Cytogenetic Characterization of Intertribal Somatic Hybrids between Brassica napus and Isatis indigotica and Backcross Progenies,” Plant Cell Reports, Vol. 28, No. 7, 2009, pp. 1105-1113. doi:10.1007/s00299-009-0712-4
Z. G. Zhao, T. T. Hu, X. H. Ge, X. Z. Du, L. Ding and Z. Y. Li, “Production and Characterization of Intergeneric Somatic Hybrids between Brassica napus and Orychophragmus violaceus and their Backcrossing Progenies,” Plant Cell Reports, Vol. 27, No. 10, 2008, pp. 1611-1621. doi:10.1007/s00299-008-0582-1
G. X. Wang, Y. Tang, H. Yan, X. G. Sheng, W. W. Hao, L. Zhang, K. Lu and F. Liu, “Production and Characterization of Interspecific Somatic Hybrids between Brassica oleracea var. botrytis and B. nigra and their Progenies for the Selection of Advanced Pre-Breeding Materials,” Plant Cell Reports, Vol. 30, No. 10, 2011, pp. 1811-1821. doi:10.1007/s00299-011-1088-9
P. Scholze, R. Kramer, U. Ryschka, E. Klocke and G. Schumann, “Somatic Hybrids of Vegetable Brassicas as Source for New Resistances to Fungal and Virus Diseases,” Euphytica, Vol. 176, No. 1, 2010, pp. 1-14. doi:10.1007/s10681-010-0205-0
O. Ovcharenko, V. Momot, N. Cherep, Y. Sheludko, I. Komarnitsky, V. Rudas and N. Kuchuk, “Transfer of Transformed Lesquerella fendleri (Gray) Wats Chloroplasts into Orychophragmus violaceus (L.) OE Schulz by Protoplast Fusion,” Plant Cell, Tissue and Organ Culture, Vol. 105, No. 1, 2011, pp. 21-27. doi:10.1007/s11240-010-9833-2
M. Wang, Z. Peng, M. Wang, W. Guo, J. Zhao, D. Zhi and G. Xia, “The Behavior of Bupleurum scorzonerifolium as a Parent of Somatic Hybrid Introgressed Lines is Associated with UV Resistance of its Chromosomes,” Russian Journal of Plant Physiology, Vol. 58, No. 4, 2011, pp. 615-621. doi:10.1134/S102144371104025X
J. Deng, H. Cui, D. Zhi, C. Zhou and G. Xia, “Analysis of Remote Asymmetric Somatic Hybrids between Common Wheat and Arabidopsis thaliana,” Plant Cell Reports, Vol. 26, No. 8, 2007, pp. 1233-1241. doi:10.1007/s00299-007-0345-4
X. Yang, X. Zhang, S. Jin, L. Fu and L. Wang, “Production and Characterization of Asymmetric Hybrids between Upland Cotton Coker 201 (Gossypium hirsutum) and Wild Cotton (G. klozschianum Anderss), “ Plant Cell Tissue and Organ Culture, Vol. 89, No. 2-3, 2007, pp. 225-235. doi:10.1007/s11240-007-9245-0
Y. Cai, F. Xiang, D. Zhi, H. Liu and G. Xia, “Genotyping of Somatic Hybrids between Festuca arundinacea Schreb. and Triticum aestivum L.,” Plant Cell Reports, Vol. 26, No. 10, 2007, pp. 1809-1819. doi:10.1007/s00299-007-0397-5
C. Yan, K. Qian, Q. Yan, X. Zhang, G. Xue, W. Huangfu, Y. Wu, Y. Zhao, Z. Xue, J. Huang, G. Xu and P. Wu, “Use of Asymmetric Somatic Hybridization for Transfer of the Bacterial Blight Resistance Trait from Oryza meyeriana L. to O. sativa L. sp japonica,” Plant Cell Reports, Vol. 22, No. 8, 2004, pp. 569-575. doi:10.1007/s00299-003-0732-4
F. Xiang, J. Wang, C. Xu and G. Xia, “The Chromosome Content and Genotype of Two Wheat Cell Lines and of their Somatic Fusion Product with Oat,” Planta, Vol. 231, No. 5, 2010, pp. 1201-1210. doi:10.1007/s00425-010-1113-1
A. Zhou and G. Xia, “Introgression of the Haynaldia villosa Genome into Gamma-Ray-Induced Asymmetric Somatic Hybrids of Wheat,” Plant Cell Reports, Vol. 24, No. 5, 2005, pp. 289-296. doi:10.1007/s00299-005-0922-3
A. Cheng and G. Xia, “Somatic Hybridisation between Common Wheat and Italian Ryegrass,” Plant Science, Vol. 166, No. 5, 2004, pp. 1219-1226. doi:10.1016/j.plantsci.2003.12.038
T. Ge, X. Lin, F. Qin, S. Yu and Y. Yu, “Protoplast Electrofusion between Common Wheat (Triticum aestivum L.) and Italian Ryegrass (Lolium multiflorum Lam.) and Regeneration of Mature Cybrids,” In Vitro Cellular & Developmental Biology-Plant, Vol. 42, No. 2, 2006, pp. 179-187. doi:10.1079/IVP2005742
A. Cheng, H. Cui and G. Xia, “Construction of a Primary RH Panel of Italian Ryegrass Genome via UV-Induced Protoplast Fusion. Plant Biology, Vol. 8, No. 5, 2006, pp. 673-679. doi:10.1055/s-2006-924276
F. Xiang, G. Xia, D. Zhi, J. Wang, H. Nie and H. Chen, “Regeneration of Somatic Hybrids in Relation to the Nuclear and Cytoplasmic Genomes of Wheat and Setaria italica,” Genome, Vol. 47, No. 4, 2004, pp. 680-688. doi:10.1139/g04-023
M. Wang, Z. Peng, L. Wang, J. Zhao, J. Che and G. Xia, “Different Rates of Chromosome Elimination in Symmetric and Asymmetric Somatic Hybridization between Festuca arundinacea and Bupleurum scorzonerifolium,” Russian Journal of Plant Physiology, Vol. 58, No. 1, 2011, pp. 133-141. doi:10.1134/S1021443710061068
C. Zhou, G. Xia, D. Zhi and Y. Chen, “Genetic Characterization of Asymmetric Somatic Hybrids between Bupleurum scorzonerifolium Willd and Triticum aestivum L.: Potential Application to the Study of the Wheat Genome,” Planta, Vol. 223, No. 4, 2006, pp. 714-724. doi:10.1007/s00425-005-0127-6
Y. Sun, Q. Xue, C. Ding, X. Zhang, L. Zhang, W. Wang and S. Ali, “Somatic Cybridization between Nicotiana tabacum and N. repanda Based on a Single Inactivation Procedure of Nuclear Donor Parental Protoplasts,” Plant Science, Vol. 168, No. 2, 2005, pp. 303-308. doi:10.1016/j.plantsci.2004.05.026
A. Sigeno, S. Hayashi, T. Terachi and H. Yamagishi, “Introduction of Transformed Chloroplasts from Tobacco into Petunia by Asymmetric Cell Fusion,” Plant Cell Reports, Vol. 28, No. 11, 2009, pp. 1633-1640. doi:10.1007/s00299-009-0763-6
J. T. Fitter, M. R. Thomas, C. Niu and R. J. Rose, “Investigation of Nicotiana tabacum (+) N. suaveolens Cybrids with Carpelloid Stamens,” Journal of Plant Physiology, Vol. 162, No.2, 2005, pp. 225-235. doi:10.1016/j.jplph.2004.02.006
M. B. Sheahan, D. W. McCurdy and R. J. Rose, “Mitochondria as a Connected Population: Ensuring Continuity of the Mitochondrial Genome during Plant Cell Dedifferentiation through Massive Mitochondrial Fusion,” The Plant Journal, Vol. 44, No 5, 2005, pp. 744-755. doi:10.1111/j.1365-313X.2005.02561.x
E. Sytnik, I. Komarnytsky, Y. Gleba and N. Kuchuk, “Transfer of Transformed Chloroplasts from Nicotiana tabacum to the Lycium barbarum Plants,” Cell Biology International, Vol. 29, No. 1, 2005, pp. 71-75. doi:10.1016/j.cellbi.2004.11.013
W. W. Guo, D. Prasad, Y. J. Cheng, P. Serrano, X. X. Deng and J. W. Grosser, “Targeted Cybridization in Citrus: Transfer of Satsuma Cytoplasm to Seedy Cultivars for Potential Seedlessness,” Plant Cell Reports, Vol. 22, No. 10, 2004, pp. 752-758. doi:10.1007/s00299-003-0747-x
Y. Cheng, M. C. de Vicente, H. Meng, W. Guo, N. Tao and X. Deng, “A Set of Primers for Analyzing Chloroplast DNA Diversity in Citrus and Related Genera,” Tree Physiology, Vol. 25, No. 6, 2005, pp. 661-672. doi:10.1093/treephys/25.6.661
M. Wang, J. Zhao, Z. Peng, W. Guo, Y. Wang, L. Wang and G. Xia, “Chromosomes Are Eliminated in the Symmetric Fusion between Arabidopsis thaliana L. and Bupleurum scorzonerifolium Willd., “ Plant Cell, Tissue and Organ Culture, Vol. 92, No. 2, 2008, pp. 121-130. doi:10.1007/s11240-007-9308-2
S. Trabelsi, R. Gargouri-Bouzid, F. Vedel, A. Nato, L. Lakhoua and N. Drira, “Somatic Fybrids between Potato Solanum tuberosum and Wild Species Solanum vernei Exhibit a Recombination in the Plastome,” Plant Cell Tissue and Organ Culture, Vol. 83, No. 1, 2005, pp. 1-11. doi:10.1007/s11240-005-3667-3
H. Yamagishi, S. Nakagawa, D. Kinoshita, A. Ishibashi and Y. Yamashita, “Somatic Hybrids between Arabidopsis thaliana and Cabbage (Brassica oleracea L.) with All Chromosomes Derived from A.thaliana and Low Levels of Fertile Seed,” Journal of the Japanese Society for Horticultural Science, Vol. 77, No. 3, 2008, pp. 277-282. doi:10.2503/jjshs1.77.277