In th e present study, five genetically modified herbicide tolerant Nicotiana tabacum cv. TAPM24 plants with a con structed vector pCAMBIA1301a carrying dehalogenase E ( dehE ) gene were compared with three non-transgenic controls using Tto 1 retrotransposon specific IRAP markers. dehE gene was originally characterized in Rhizobium sp. and it pro duce d an enzyme which degrade d the Dalapon herbicide. IRAP protocol was applied on transgenic and non-transgenic plants to investigate the retrotransposon based genetic variation which may appear during transformation. Polymor phism rates were calculated as 0 % - 20% from IRAP-PCR products among all plant samples. These results show that transformation of tobacco plant with the dehE gene may cause Tto 1 retrotransposon alterations appearing as different band profiles. The findings are expected to contribute to genetic engineering studies to obtain better results and also to understand how transposons contribute to features such as transgenesis. In our knowledge, this is one of the first ex perimental data of transgenic N. tabacum engineered with dehE gene originated Rhizobium sp. in terms of retrotranpo son based variation.
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