The present study describes a direct shoot regeneration-based micropropagation procedure for Vanilla planifolia . Two types of explant ( i.e . shoot apex and stem nodal segment) were screened for their shoot induction potential following a three-month treatment with 6-benzylaminopurine (BAP) and α -Naphthaleneacetic acid (NAA). Results indicated that the shoot apices were poor candidates for shoot induction whereas the stem nodal segments showed potential for shoot initiation at a rate of up to 6 shoots/explant. Stem nodal segments were the most responsive as shoots formed (55 shoots) directly following treatments with 1 mg/L BAP at half strength MS medium after the third subculture. In addition, more shoots were produced on solid medium treatments compared to the liquid medium treatments in two strengths of me diums tested. Regenerated plantlets derived from the 1 mg/L of BAP treatment w ere induced to root following a one month culture in growth regulator-free MS medium. There was 90% survival rate of the rooted plantlets after acclima tization in the greenhouse. The findings in the present study would be helpful for large-scale mass propagation of Va nilla planifolia using this simple and efficient protocol.
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