Influence of Desiccation Time on Survival and Regeneration of Embryonic Axes of Groundnut (<i>Arachis hypogaea</i> L.) Immersed in Liquid Nitrogen — Oak Academic Publishing
Research ArticleOpen AccessGoogle Scholar indexed
Influence of Desiccation Time on Survival and Regeneration of Embryonic Axes of Groundnut (<i>Arachis hypogaea</i> L.) Immersed in Liquid Nitrogen
Department of Plant Science, Ahmadu Bello University, Zaria, Nigeria.
,
Department of Plant Science, Ahmadu Bello University, Zaria, Nigeria.
,
Department of Plant Science, Ahmadu Bello University, Zaria, Nigeria.
,
Department of Plant Science, Ahmadu Bello University, Zaria, Nigeria.
1 Department of Plant Science, Ahmadu Bello University, Zaria, Nigeria.
2 Department of Plant Science, Ahmadu Bello University, Zaria, Nigeria.
3 Department of Plant Science, Ahmadu Bello University, Zaria, Nigeria.
4 Department of Plant Science, Ahmadu Bello University, Zaria, Nigeria.
Cryopreservation , the storage of biological materials in liquid nitrogen (LN) , is a useful method for long term conserva tion of plant germplasm. This study was carried out with the objective of establishing an efficient desiccation technique for successful cryopreservation and recovery of embryonic axes of groundnut. Embryonic axes of four groundnut ( Ara chis hypogaea L.) genotypes were evaluated. The excised embryonic axes were dehydrated by air current of a laminar air flow cabinet for different duration (0, 1, 2, 3, 4 & 5 hrs) before being plunged in LN (-196℃ ) and held for 1 hr. Samples were thawed in water bath at 40℃ for 2 min, thereafter cultured on MS medium supplemented with 15 mg/L BAP for recovery. Highest survival (96.67% - 100%) and shoot formation (91.67% - 96.67%) were obtained at an av erage moisture content of 17% after 4 - 5 hr desiccation. Among the genotypes evaluated , Samnut 22 and Samnut 23 recorded the highest survival and shoot formation. This technique therefore appears promising for cryopreservation of groundnut germplasm.
J. B. Morris, S. Dunn and R. N. Pittman, “Plant Recovery from Embryonic Axes of Deteriorated Peanut Seed for Germplasm Renewal,” Peanut Science, Vol. 22, No. 1, 1995, pp. 66-70. doi:10.3146/pnut.22.1.0015
M. A. Perez and J. A. Arguello, “Deterioration of Peanut (Arachis hypogaea L. cv. Florman) Seed under Natural and Accelerated Aging,” Seed Science and Technology, Vol. 23, No. 2, 1995, pp. 439-445.
F. Engelmann, “Plant Cryopreservation: Progress and Prospects,” In Vitro Cellular Developmental Biology-Plant, Vol. 40, No. 5, 2004, pp. 427-433. doi:10.1079/IVP2004541
N. R. F. Castillo, N. V. Bassil, S. Wada and B. M. Reed, “Genetic Stability of Cryopreserved Shoot Tips of Rubus Germplasm,” In Vitro Cellular and Developmental Bioliogy-Plant Vol. 46, No. 3, 2010, pp. 246-256.
M. N. Normah and A. M. Makeen, “Cryopreservation of Excised Embryos and Embryonic Axes,” In: B. M. Reed, Ed., Plant Cryopreservation: A Practical Guide, Vol. 18, 2008, pp. 211-220. doi:10.1007/978-0-387-72276-4_10
A. M. Makeen, M. N. Normah, S. Dussert and M. M. Clyde, “Cryopreservation of Whole Seeds and Excised Embryonic Axes of Citrus suhuensis cv. Limau Langkat in Accordance to Their Desiccation Sensitivity,” Cryo Letters, Vol. 26, No. 4, 2005, pp. 259-268.
O. M. Alzoubi and M. N. Normah, “Desiccation Sensitivity of Cryopreservation of Excised Embryonic axes of Citrus suhuiensis cv. Limau Madu, Citrumelo [Citrus paradisi Macf. x Poncirus trifoliate (L.) Raf] and Fortunella polyandra,” Cryo Letters, Vol. 33, No. 3, 2012, pp. 240-250.
R. Chaudhury and K. P. S. Chandel, “Cryopreservation of Embryonic Axes of Almond (Prunus amygdalus Batsch.) Seeds,” Cryo Letters, Vol. 16, No. 1, 1995, pp. 51-56.
B. Wen and S. Song, “Acquisition of Cryotolerance in Maize Embryos during Seed Development,” Cryo Letters, Vol. 28, No. 2, 2007, pp. 109-118.
T. Murashige and F. Skoog, “A Revised Medium for Rapid Growth and Bioassays with Tobacco Tissue Cultures,” Physiologia Plantarum, Vol. 15, No. 43, 1962, pp. 473-497. doi:10.1111/j.1399-3054.1962.tb08052.x
D. B. Duncan, “Multiple Range and Multiple F-Test,” Biometrics, Vol. 11, No. 1, 1955, pp. 1-42. doi:10.2307/3001478
R. F. Gagliardi, G. P. Pacheco, J. F. M. Vall and E. Mansur, “Cryopreservation of Cultivated and Wild Arachis Species Embryonic Axes Using Desiccation and Vitrification Methods,” Cryo Letters, Vol. 23, No. 1, 2002, pp. 61-68.
M. Azimi, C. O’Brien, S. Ashmore and R. Drew, “Cryopreservation of Papaya Germplasm,” In: W. C. Chang and R. Drew, Eds., IInd IS on Biotechnology of Tropical & Subtropical Species, 2005, pp. 43-50. wwwlib.teiep.gr/stories/acta/Acta 692/692_4.pdf
D. Dumet, F. Engelmann, N. Chabrillange and Y. Duval, “Cryopreservation of Oil Palm (Elaeis guineensis Jacq.) Somatic Embryos Involving a Desiccation Step,” Plant Cell Reports, Vol. 12, No. 6, 1993, pp. 352-355. doi:10.1007/BF00237434
A. Halmagyi, S. Valimareanu, A. Coste, C. Deliu and V. Isac, “Cryopreservation of Malus Shoots Tips and Subsequent Plant Regeneration,” Romanian Biotechnological Letters, Vol. 15, No. 1, 2010, pp. 79-85.
E. A. Ozudogru, Y. Ozden-Tokatli, F. Gumusel, C. Benelli and M. Lambardi, “Development of a Cryopreservation Procedure for Peanut (Arachis hypogaea L.) Embryonic Axes and Its Application to Local Turkish Germplasm,” Advances in Horticultural Science, Vol. 23, No. 1, 2009, pp. 41-48.
R. E. L. Percy, N. J. Livingston, A. Jonathan, J. A. Moran and P. Von Aderkas, “Desiccation, Cryopreservation and Water Relations Parameters of White Spruce (Picea glauca) and Interior Spruce (Picea glauca × Engelmannii Complex) Somatic Embryos,” Tree Physiology, Vol. 21, No. 18, 2001, pp. 1303-1310. doi:10.1093/treephys/21.18.1303
L. Taiz and E. Zeiger, “Plant Physiology: Auxins,” 3rd Edition, Macmillan Publishing Co., New York, 2003, p. 544.
X. Xue-Xuan, S. Hong-Bo, M. Yuan-Yuan, X. Gang, S. Jun-Na, G. Dong-Gang and R. Cheng-Jiang, “Biotechnological Implications from Abscisic Acid (ABA) Roles in Cold Stress and Leaf Senescence as an Important Signal for Improving Plant Sustainable Survival under Abiotic-Stressed Conditions,” Critical Reviews in Biotechnology, Vol. 30, No. 3, 2010, pp. 222-230. doi:10.3109/07388551.2010.487186
B. Wen and S. Song, “Acquisition and Loss of Cryotolerance in Livistona chinensis Embryos during Seed Development,” Cryo Letters, Vol. 28, No. 4, 2007, pp. 291-302.
L. A. Mroginsky, K. K. Kartha and J. P. Shyluk, “Regeneration of Peanut (Arachis hypogaea L) Plantlets in Vitro Culture of Immature Leaves,” Canadian Journal of Botany, Vol. 59, No. 5, 1981, pp. 826-830.
P. Banerjee, S. Maity, S. S. Maiti and N. Banerjee, “Influence of Genotype on in Vitro Multiplication Potential of Arachis hypogaea L.,” Acta Botanica Croatica, Vol. 66, No. 1, 2007, pp. 15-23.
M. M. Abdulmalik, I. S. Usman, J. D. Olarewaju and D. A. Aba, “Effect of Naphthalene Acetic Acid (NAA) on in Vitro Rooting of Regenerated Microshoots of Groundnut (Arachis hypogaea L.),” Bayero Journal of Pure and Applied Sciences, Vol. 5, No. 2, 2012, pp. 128-131.
I. S. Usman and M. M. Abdulmalik, “Cryopreservation of Embryonic Axes of Maize (Zea maize L.) by Vitrification Protocol,” African Journal of Biotechnology, Vol. 9, No. 52, 2010, pp. 8955-8957.