Low-Density Co-Inoculation of Myanmar <i>Bradyrhizobium yuanmingense</i> MAS34 and <i>Streptomyces griseoflavus</i> P4 to Enhance Symbiosis and Seed Yield in Soybean Varieties — Oak Academic Publishing
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Low-Density Co-Inoculation of Myanmar <i>Bradyrhizobium yuanmingense</i> MAS34 and <i>Streptomyces griseoflavus</i> P4 to Enhance Symbiosis and Seed Yield in Soybean Varieties
Plant Nutrition Laboratory, Graduate School of Bioresource and Bioenvironmental Sciences, Faculty of Agriculture, Kyushu University, Fukuoka, Japan
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Plant Nutrition Laboratroy, Division of Molecular Biosciences, Department of Biosciences & Biotechnology, Faculty of Agriculture, Kyushu University, Fukuoka, Japan.
1 Plant Nutrition Laboratory, Graduate School of Bioresource and Bioenvironmental Sciences, Faculty of Agriculture, Kyushu University, Fukuoka, Japan
2 Plant Nutrition Laboratroy, Division of Molecular Biosciences, Department of Biosciences & Biotechnology, Faculty of Agriculture, Kyushu University, Fukuoka, Japan.
This study examined whether low-density co-inoculation of Myanmar Bradyrhizobium yuanmingense strain MAS34 and Streptomyces griseoflavus P4 would enhance nodulation, N 2 fixation, and seed yield in two soybean varieties. A field experiment was conducted during the July to November 2012 growing season at Kyushu University Farm, Japan, using a split-plot design with three replications and the following four treatments: T1, an uninoculated treatment with peat moss (uninoculated); T2, a single inoculation with S. griseoflavus P4 (P4); T3, a single inoculation of B. yuanmin gense MAS34 (MAS34); and T4, a dual inoculation of P4 with MAS34 (P4 + MAS34). Two varieties of soybean, Yezin-3 ( Rj 4 ) and Yezin-6 (non -Rj ), were used. The N 2 fixation activity of soybean was evaluated by the relative ureide method using xylem solute from root bled sap at the early pod-fill stage (R3.5). Dry matter production, N 2 fixation, and seed yield were significantly ( P < 0.01) different between the inoculated treatments. The effect of variety was also sig nificant ( P < 0.05) for nodule dry weight at the V6 stage, percentage of N derived from the atmosphere at the R3.5 stage, and seed yield at the maturity stage. The number of nodules on the tap roots was significantly higher in Yezin-3 than in Yezin-6. The single inoculation of P4 did not have a significant effect on dry matter production, N 2 fixation, and seed yield in either soybean variety. The dry matter production, relative ureide index, percentage of N derived from the at mosphere, and seed yield were significantly ( P < 0.01) enhanced by a single inoculation of MAS34 in Yezin-3 and by dual inoculation of P4 + MAS34 in Yezin-6. These results indicate that low inoculum concentrations (10 5 cells seed - 1 ) increase N 2 fixation and seed yield in these soybean varieties under open field conditions. Myanmar B. yuanmingense MAS34 and S. griseoflavus P4 are expected to be useful biofertilizers for soybean production.
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