The Effects of Auxins and Cytokinin on Growth and Development of (<i>Musa</i> sp.) Var. “Yangambi” Explants in Tissue Culture — Oak Academic Publishing
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The Effects of Auxins and Cytokinin on Growth and Development of (<i>Musa</i> sp.) Var. “Yangambi” Explants in Tissue Culture
School of Life Sciences, Nelson Mandela African Institution of Science and Technology, Arusha, Tanzania
,
Mikocheni Agricultural Research Institute, Dar es Salaam, Tanzania
,
School of Life Sciences, Nelson Mandela African Institution of Science and Technology, Arusha, Tanzania
1 School of Life Sciences, Nelson Mandela African Institution of Science and Technology, Arusha, Tanzania
2 Mikocheni Agricultural Research Institute, Dar es Salaam, Tanzania
3 School of Life Sciences, Nelson Mandela African Institution of Science and Technology, Arusha, Tanzania
The aim of this study was to investigate the effects of concentration of different growth regulators (auxins and cytokinins) on growth and development of banana shoot tips cultured in vitro . Explants were taken from young suckers of field grown plants of var. “ Yangambi ” . The shoot tips were cultured on MS media supplemented with different concen trations of BAP (0, 2, 4, 6 and 8 mg/l) with or without IAA at concentration of 0.34 mg/l. At the rooting phase , the me dia was supplemented with different concentrations of IBA (0.1, 0.5, 1.0, 1.5 and 2.0 mg/l) with or without BAP at concentration of 0.2 mg/l. The results indicated that 6.0 mg/l BAP significantly increased the number of shoots formed and the interaction of 6 mg/l BAP with 0.35 mg/l IAA significantly increased the fresh weight. For rooting, 2.0 mg/l IBA was more efficient in number and length of roots produced than all other treatments.
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