Expression Analysis of Two Genes Coding for Trehalose-6-Phosphate Synthase (TPS), in Sugarcane (<i>Saccharum</i> spp.) under Water Stress — Oak Academic Publishing
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Expression Analysis of Two Genes Coding for Trehalose-6-Phosphate Synthase (TPS), in Sugarcane (<i>Saccharum</i> spp.) under Water Stress
Institute of Genetics and Biochemistry, Federal University of Uberlandia, Uberlandia, Brazil
,
Center of Education, Communication and Arts, Western Paraná State University, Cascavel, Brazil
,
Biotechnology Unit, UNAERP, Ribeir?o Preto, Brazil
,
Biotechnology Unit, UNAERP, Ribeir?o Preto, Brazil
1 Institute of Genetics and Biochemistry, Federal University of Uberlandia, Uberlandia, Brazil
2 Center of Education, Communication and Arts, Western Paraná State University, Cascavel, Brazil
3 Biotechnology Unit, UNAERP, Ribeir?o Preto, Brazil
4 Biotechnology Unit, UNAERP, Ribeir?o Preto, Brazil
The accumulation of trehalose ( α -D-glucopyranosyl-[1,1]- α -D-glucopyranoside), a sugar with osmoprotectant properties, is very common in microorganisms, invertebrates and in resurrection plants. However, in the majority of higher plants, it is found in trace amounts. Trehalose is synthesized from the UDP-glucose and glucose-6-phosphate in a two-step process with two enzymes, trehalose-6-phosphate synthase or TPS (EC 2.4.1.15 and EC 2.4.1.36) and trehalose-6-phosphate phosphatase or TPP (EC 3.1.3.12). The trehalose-6-phosphate synthase and its product of the trehalose-6-phosphate (T6P) are probable signaling molecules in the carbohydrate metabolism, contributing to enhancing the plants tolerance to water stress. Water scarcity is one of the most important factors that influence productivity in sugarcane ( Saccharum spp.) and it activates a cascade of metabolic events and necessary morphologic changes for the survival of the plant under stress. Here we show the in silico expression study of TPS in different libraries from the SUCEST project. Our results showed that the TPS genes are present in all tissues and that they are divided into two subfamilies (class I and II). It is shown that STPS1 belongs to the class I, therefore, it does not have an active phosphatase (TPP) domain, whereas, the STPS2 has an active TPP domain (class II) determined by the presence of phosphatase boxes. Expression analyses based on the semi-quantitative method of the reverse transcription polymerase chain reaction (RT-PCR) show that the STPS1 gene is up-regulated in the tolerant cultivar under stress and down-regulated in susceptible plants. The STPS2 gene does not show considerable variations in the expression levels under the same treatments. The discovery of active genes such as STPS1 and STPS2 in plants under water stress, contributes for the concerning about the cascade of responses in plants under water deficit and points out to target genes for plant breeding.
E. Cabib and F. L. Leloir, “The Biosynthesis of Trehalose-6-Phosphate,” Journal of Biological Chemistry, Vol. 31, 1958, pp. 259-275.
J. M. Thevelein, “Regulation of Trehalose Metabolism and Its Relevance to Cell Growth and Function,” In: R. Brambl and G. A. Marzluf, Eds., The Mycota, Springer, Berlin-Verlag, 1996, pp. 395-420.
O. Kandror, A. DeLeon and A. L. Goldberg, “Trehalose Synthesis Is Induced upon Exposure of Escherichia coli to Cold and Is Essential for Viability at Low Temperatures,” Proceedings of the National Academy of Sciences, Vol. 99, No. 15, 2002, pp. 9727-9732. http://dx.doi.org/10.1073/pnas.142314099
Van Laere, “Trehalose, Reserve and/or Stress Metabolite?” FEMS Microbiology Reviews, Vol. 63, No. 3, 1989, pp. 201-210.
A. Wiemken, “Trehalose in Yeast, Stress Protectant rather Than Reserve Carbohydrate,” Antonie van Leeuwenhoek, Vol. 58, No. 3, 1990, pp. 209-217. http://dx.doi.org/10.1007/BF00548935
G. Bianchi, A. Gamba, R. Limiroli, N. Pozzi, R. Elster, F. Salamini and D. Bartels, “The Unusual Sugar Composition in Leaves of the Resurrection Plant Myrothamnus flabellifolia,” Physiologia Plantarum, Vol. 87, No. 2, 1993, pp. 223-226. http://dx.doi.org/10.1111/j.1399-3054.1993.tb00146.x
P. M. Drennan, M. T. Smith, D. Goldsworthy and J. van Staden, “The Occurrence of Trehalose in the Leaves of the Desiccation Tolerant Angiosperm Myrothamnus flabellifolius Welw,” Journal of Plant Physiology, Vol. 142, No. 4, 1993, pp. 493-496. http://dx.doi.org/10.1016/S0176-1617(11)81257-5
M. A. Blazquez, E. Santos, C. L. Flores, J. M. MartinezZapater, J. Salinas and C. Gancedo, “Isolation and Characterization of the Arabidopsis TPS1 Gene, Encoding Trehalose-6-Phosphate Synthase,” Plant Journal, Vol. 13, No. 5, 1998, pp. 685-689. http://dx.doi.org/10.1046/j.1365-313X.1998.00063.x
A. Leyman, P. Van Dijck and J. M. Thevelein, “An Unexpected Plethora of Trehalose Biosynthesis Genes in Arabidopsis Thaliana,” Trends in Plant Science, Vol. 6, 2001, pp. 510-513. http://dx.doi.org/10.1016/S1360-1385(01)02125-2
G. Vogel, O. Fiehn, L. J. R. D. Bressel, T. Boller, A. Wiemken, R. A. Aeschbacher and A. Wingler, “Trehalose Metabolism in Arabidopsis: Occurrence of Trehalose and Molecular Cloning and Characterization of Trehalose-6-Phosphate Synthase Homologues,” Journal of Experimental Botany, Vol. 52, No. 362, 2001, pp. 1817-1826. http://dx.doi.org/10.1093/jexbot/52.362.1817
O. Goddijn and S. Smeekens, “Sensing Trehalose Biosynthesis in Plants,” Plant Journal, Vol. 14, No. 2, 1998, pp. 143-146. http://dx.doi.org/10.1046/j.1365-313X.1998.00140.x
S. A. Kosmas, A. Argyrokastritis, M. G. Loukas, E. Eliopoulos, S. Tsakas and P. J. Kaltsikes, “Isolation and Characterization of Drought-Related Trehalose 6-Phosphate-Synthase Gene from Cultivated Cotton (Gossypiumhirsutum L.),” Planta, Vol. 223, No. 2, 2006, pp. 329-339. http://dx.doi.org/10.1007/s00425-005-0071-5
C. Thaller, S. Schippa and G. M. Rossolini, “Conserved Sequence Motifs among Bacterial, Eukaryotic and Archaeal Phosphatases That Define a New Phosphohydrolase Superfamily,” Protein Science, Vol. 7, No. 7, 1998, pp. 1651-1656. http://dx.doi.org/10.1002/pro.5560070722
P. J. Eastmond, A. J. H. van Dijken, M. Spielman, A. Kerr, A. F. Tissier, H. G. Dickinson, J. D. G. Jones, S. C. Smeekens and I. A. Graham, “Trehalose-6-Phosphate Synthase 1, Which Catalyses the First Step in Trehalose Synthesis, Is Essential for Arabidopsis Embryo Maturation,” Plant Journal, Vol. 29, No. 2, 2002, pp. 225-235. http://dx.doi.org/10.1046/j.1365-313x.2002.01220.x
H. Schluepmann, T. Pellny, A. van Dijken, S. Smeekens and M. Paul, “Trehalose 6-Phosphate Is Indispensable for Carbohydrate Utilization and Growth in Arabidopsis thaliana,” Proceedings of the National Academy of Sciences, Vol. 100, No. 11, 2003, pp. 6849-6854. http://dx.doi.org/10.1073/pnas.1132018100
H. Schluepmann, A. van Dijken, M. Aghdasi, B. Wobbes, M. Paul and S. Smeekens, “Trehalose Mediated Growth Inhibition of Arabidopsis Seedlings Is Due to Trehalose-6-Phosphate Accumulation,” Plant Physiology, Vol. 135, No. 2, 2004, pp. 879-890. http://dx.doi.org/10.1104/pp.104.039503
J. van Dijken, H. Schluepmann and S. C. Smeekens, “Arabidopsis Trehalose-6-Phosphate Synthase 1 Is Essential for Normal Vegetative Growth and Transition to Flowering,” Plant Physiology, Vol. 135, No. 2, 2004, pp. 969-977. http://dx.doi.org/10.1104/pp.104.039743
B. Zang, H. Li, W. Li, X. W. Deng and X. Wang, “Analysis of Trehalose-6-Phosphate Synthase (TPS) Complexes in Rice,” Plant Molecular Biology, Vol. 76, No. 6, 2011, pp. 507-522. http://dx.doi.org/10.1007/s11103-011-9781-1
N. Avonce, B. Leyman, J. O. Gallardo-Mascorro, P. Van Dijck, J. M. Thevelein and G. Iturriaga, “The Arabidopsis Trehalose-6-P Synthase AtTPS1 Gene is a Regulator of Glucose, Abscisic Acid, and Stress Signaling,” Plant Physiology, Vol. 136, No. 3, 2004, pp. 3649-3659. http://dx.doi.org/10.1104/pp.104.052084
M. Almeida, E. Villalobos, S. S. Araújo, B. Leyman, P. van Dijk, L. Alfaro-Cardoso, P. S. Fevereiro, J. Torné and D. M. Santos, “Transformation of Tobacco with an Arabidopsis thaliana Gene Involved in Trehalose Biosynthesis Increases Tolerance to Several Abiotic Stresses,” Euphytica, Vol. 146, 2005, pp. 165-176. http://dx.doi.org/10.1007/s10681-005-7080-0
S. N. Chary, G. R. Hicks, Y. G. Choi, D. Carter and N. V. Raikhel, “Trehalose-6-Phosphate Synthase/Phosphatase Regulates Cell Shape and Plant Architecture in Arabidopsis,” Plant Physiology, Vol. 146, No. 1, 2008, pp. 97-107. http://dx.doi.org/10.1104/pp.107.107441
S. Zhang, B. Yang, C. Feng, R. Chen, J. Luo, W. Cai and F. Liu, “Expression of the Grifola frondosa Trehalose Synthase Gene and Improvement of Drought-Tolerance in Sugarcane (Saccharum officinarum L.),” Journal of Integrative Plant Biology, Vol. 48, No. 4. 2006, pp. 453-459. http://dx.doi.org/10.1111/j.1744-7909.2006.00246.x
E. A. Bray, “Molecular Responses to Water Deficit,” Plant Physiology, Vol. 103, No. 4, 1993, pp. 1035-1040.
G. C. Dedemo, F. A. Rodrigues, P. G. Roberto, C. B. Neto, S. C. França and S. M. Zingaretti, “Osmoprotection in Sugarcane under Water Deficit Conditions,” Plant Stress, Vol. 7, No. 1, 2013, pp. 1-7.
A. D'Hont, L. Grivet, P. Feldmann, S. Rao, N. Berding and J. C. Glaszmann, “Characterisation of the Double Genome Structure of Modern Sugarcane Cultivars (Saccharum spp.) by Molecular Cytogenetics,” Molecular Genetics and Genomics, Vol. 250, No. 4, 1996, pp. 405-413.
L. Vettore, F. R. da Silva, E. L. Kemper and P. Arruda, “The Libraries That Made SUCEST,” Genetics and Molecular Biology, Vol. 24, No. 1-2, 2001, pp. 1-4. http://dx.doi.org/10.1590/S1415-47572001000100002
X. Huang, “A Contig Assembly Program Based on Sensitive Detection of Fragment Overlaps,” Genomics, Vol. 14, 1992, pp. 18-25. http://dx.doi.org/10.1016/S0888-7543(05)80277-0
S. F. Altschul, W. Gish, W. Miller, E. W. Myers and D. J. Lipman, “Basic Local Alignment Search Tool,” Journal of Molecular Biology, Vol. 215, No. 3, 1990, pp. 403-410.
T. Rombel, K. F. Sykes, S. Rayner and A. S. Johnston, “ORF-FINDER: A Vector for High-Throughput Gene Identification,” Gene, Vol. 282, No. 1-2, 2002, pp. 33-41. http://dx.doi.org/10.1016/S0378-1119(01)00819-8
A. Marchler-Baue and S. H. Bryant, “CD-Search: Protein Domain Annotations on the Fly,” Nucleic Acids Research, Vol. 32, Suppl. 2, 2004, pp. 327-331. http://dx.doi.org/10.1093/nar/gkh454
M. J. D. Thompson, D. G. Higgins and T. J. Gibson, “CLUSTAL W: Improving the Sensitivity of Progressive Multiple Sequence Alignment through Sequence Weighting, Position-Specific Gap Penalties and Weight Matrix Choice,” Nucleic Acids Research, Vol. 22, No. 22, 1994, pp. 4673-4680. http://dx.doi.org/10.1093/nar/22.22.4673
N. Saitou and M. Nei, “The Neighbour-Joining Method: A New Method for Reconstructing Phylogenetic Trees,” Molecular Biology and Evolution, Vol. 4, No. 4, 1987, pp. 406-425
M. H. R. Pramanik and R. Imai, “Functional Identification of a Trehalose 6-Phosphate Phosphatase Gene That Is Involved in Transient Induction of Trehalose Biosynthesis during Chilling Stress in Rice,” Plant Molecular Biology, Vol. 58, 2005, pp. 751-762. http://dx.doi.org/10.1007/s11103-005-7404-4
W. Jiang, F.-L. Fu, S.-Z. Zhang, L. Wu and W.-C. Li, “Cloning and Characterization of Functional Trehalose-6-Phosphate Synthase Gene in Maize,” Journal of Plant Biology, Vol. 53, No. 2, 2010, pp. 134-141. http://dx.doi.org/10.1007/s12374-010-9098-7
N. Avonce, A. Mendoza-Vargas, E. Morett and G. Iturriaga, “Insights on the Evolution of Trehalose Biosynthesis,” BMC Evolutionary Biology Vol. 6, No. 1, 2006, p. 109. http://dx.doi.org/10.1186/1471-2148-6-109
K. Garg, J. K. Kim, T. G. Owens, A. P. Ranwala, Y. D. Choi, L. V. Kochian and R. J. Wu, “Trehalose Accumulation in Rice Plants Confers High Tolerance Levels to Different Abiotic Stresses,” Proceedings of the National Academy of Sciences USA, Vol. 99, No. 25, 2002, pp. 15898-15903. http://dx.doi.org/10.1073/pnas.252637799
C. Jang, S. Oh, J. S. Seo, W. B. Choi, S. Y. Song, C. H. Kim, Y. S. Kim, H. S. Seo, Y. D. Choi, B. H. Nahm and J. K. Kim, “Expression of a Bifunctional Fusion of the Escherichia coli Genes for Trehalose-6-Phosphate Synthase and Trehalose-6-Phosphate Phosphatase in Transgenic Rice Plants Increases Trehalose Accumulation and Abiotic Stress Tolerance without Stunting Growth,” Plant Physiology, Vol. 131, No. 2, 2003, pp. 516-524. http://dx.doi.org/10.1104/pp.007237
Z. Z. Wang, S. Z. Zhang, B. P. Yang and Y. R. Li, “Trehalose Synthase Gene Transfer Mediated by Agrobacterium tumefaciens Enhances Resistance to Osmotic Stress in Sugarcane,” Scientia Agricultura Sinica, Vol. 36, No. 2, 2003, pp. 140-146.
T. K. Pellny, O. Ghannoum, J. P. Conroy, H. Schluepmann, S. Smeekens, J. Andralojc, K. P. Krause, O. Goddijn and M. J. Paul, “Genetic Modification of Photosynthesis with E. coli Genes for Trehalose Synthesis,” Plant Biotechnology Journal, Vol. 2, No. 1, 2004, pp. 71-82. http://dx.doi.org/10.1111/j.1467-7652.2004.00053.x
J. E. Lunn, R. Feil, J. H. M. Hendriks, Y. Gibon, R. Morcuende, D. Osuna, W. R. Scheible, O. Carillo, M. R. Hajirezaei and M. Stitt, “Sugar-Induced Increases in Trehalose 6-Phosphate Are Correlated with Redox Activation of ADP Glucose Pyrophosphorylase and Higher Rates of Starch Synthesis in Arabidopsis thaliana,” Biochemical Journal, Vol. 397, No. 1, 2006, pp. 139-148. http://dx.doi.org/10.1042/BJ20060083
J. E. Harthill, S. E. Meek, N. Morrice, M. W. Peggie, J. Borch, B. H. Wong and C. Mackintosh, “Phosphorylation and 14-3-3 Binding of Arabidopsis Trehalose-Phosphate Synthase 5 in Response to 2-Deoxyglucose,” Plant Journal, Vol. 47, No. 2, 2006, pp. 211-223. http://dx.doi.org/10.1111/j.1365-313X.2006.02780.x
N. G. Halford, S. Hey, D. Jhurreea, S. Laurie, R. S. McKibbin, M. Paul and Y. Zhang, “Metabolic Signalling and Carbon Partitioning: Role of Snf1-Related (SnRK1) Protein Kinase,” Journal of Experimental Botany, Vol. 54, No. 382, 2003, pp. 467-475. http://dx.doi.org/10.1093/jxb/erg038