Effect of Ambient UV-B on Stomatal Density, Conductance and Isotope Discrimination in Four Field Grown Soybean [<i>Glycine max</i> (L.) Merr.] Isolines — Oak Academic Publishing
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Effect of Ambient UV-B on Stomatal Density, Conductance and Isotope Discrimination in Four Field Grown Soybean [<i>Glycine max</i> (L.) Merr.] Isolines
USDA-ARS#, Cropping Systems Research Laboratory, Lubbock, USA
,
USDA-ARS, Food Components and Health Laboratory, Beltsville, USA
,
Department of PLSA, College of Agriculture and Natural Resources, University of Maryland, College Park, USA
1 USDA-ARS#, Cropping Systems Research Laboratory, Lubbock, USA
2 USDA-ARS, Food Components and Health Laboratory, Beltsville, USA
3 Department of PLSA, College of Agriculture and Natural Resources, University of Maryland, College Park, USA
An experiment was designed to test whether ambient levels of UV-B radiation affect stomatal development, decrease stomatal density, and lead to increased water-use efficiency (WUE). Soybean [ Glycine max (L.) Merr.] isolines with different stomatal distribution and flavonol expression patterns were field grown under shelters that either transmitted or blocked solar UV-B. All isolines exposed to solar UV-B accumulated higher concentrations of UV-screening phenolic pigments but other responses were isoline dependent. Solar UV-B decreased stomatal density and conductance in isolines expressing a unique branched kaempferol triglycoside. Decreased stomatal density was associated with increased season-long WUE and decreased internal CO 2 concentration of leaf (estimated by δ 13 C discrimination). We concluded that photomorphogenic responses to UV-B affected stomatal density and WUE in field grown soybean; but that the magnitude and direction of these response were associated with isogenic pleiotropic differences in stomatal distribution and pigment expression. UV-B radiation had no effect on biomass accumulation or yield in a cultivar expressing only trace levels of kaempferol suggesting that flavonol expression is not prerequisite to UV-B tolerance.
KeywordsSoybeanUV-BDrought StressWater Use EfficiencyIsotope DiscriminationPhotomorphogenesis
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