Influence of Bavistin, Cefotoxime, Kanamycin and Silver Thiosulphate on Plant Regeneration of <i>Solanum viarum</i> (Dunal)—An Important Anticancer Medicinal Plant — Oak Academic Publishing
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Influence of Bavistin, Cefotoxime, Kanamycin and Silver Thiosulphate on Plant Regeneration of <i>Solanum viarum</i> (Dunal)—An Important Anticancer Medicinal Plant
Department of Biotechnology, Dravidian University, Kuppam, In-dia
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Department of Biotechnology, Dravidian University, Kuppam, In-dia
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Department of Biotechnology, Dravidian University, Kuppam, In-dia
1 Department of Biotechnology, Dravidian University, Kuppam, In-dia
2 Department of Biotechnology, Dravidian University, Kuppam, In-dia
3 Department of Biotechnology, Dravidian University, Kuppam, In-dia
The effect s of bavistin, cefotoxime and kanamycin along with the ethylene inhibitor silver thiosulphate on in vitro regeneration using the axillary buds of Solanum viarum (D.) ha ve been investigated. Axillary bud explants when cultured on MS medium supplemented with bavistin (100 mg/l) maximum number of shoots (4.21 ± 0.83) w ere observed. In a separate experiment , bavistin along with growth regulators such as BAP and TDZ w as added to MS medium containing sucrose (3%). Maximum number of shoots was obtained (5.55 ± 0.28) in MS medium supplemented with bavistin (100 mg/l) and BAP (2.0 mg/l). Cefotoxime and kanamycin (10 - 150 μM/L) w ere added to MS medium supplemented with BAP (2.0 mg/l) with sucrose (3%) hav ing shown the maximum shoot regeneration (4.38 ± 0.45 and 3.4 ± 0.48) at 80 μM/L and 50 μM/L respectively. Similarly the regeneration me dium was supplemented with (5.0 - 100 μM/L) of STS. Maximum number of shoots ( 4.75 ± 0.43) was seen with 40 μM/L. These plantlets were further maintained for root emergence on a rooting medium supplemented with growth regulators such as (0.5 - 2.0 mg/l) IAA, IBA and NAA. In MS medium supplemented with IBA (1.0 mg/l) , maximum number of roots (24.3 ± 0.31) was seen with 100% regeneration. The rooted plants were acclimatized and transferred to field plots with (95%) of plant surviving in the field.
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