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Isolation and Characterization of Novel Microsatellite Markers in Walnut (<i>Juglans regia</i> L.)
Department of Agronomy and Plant Breeding, Faculty of Agriculture, University of Zabol, Zabol, Iran; Agricultural Biotech- nology Research Institute of Iran (ABRII), Karaj, Iran
Agricultural Biotech- nology Research Institute of Iran (ABRII), Karaj, Iran
Department of Agronomy and Plant Breeding, Faculty of Agri-culture, University of Zabol, Zabol, Iran
Agricultural Biotech- nology Research Institute of Iran (ABRII), Karaj, Iran
Faculty of Agriculture, University of Tehran, Karaj, Iran
Agricultural Biotech- nology Research Institute of Iran (ABRII), Karaj, Iran
- 1 Department of Agronomy and Plant Breeding, Faculty of Agriculture, University of Zabol, Zabol, Iran; Agricultural Biotech- nology Research Institute of Iran (ABRII), Karaj, Iran
- 2 Agricultural Biotech- nology Research Institute of Iran (ABRII), Karaj, Iran
- 3 Department of Agronomy and Plant Breeding, Faculty of Agri-culture, University of Zabol, Zabol, Iran
- 4 Agricultural Biotech- nology Research Institute of Iran (ABRII), Karaj, Iran
- 5 Faculty of Agriculture, University of Tehran, Karaj, Iran
- 6 Agricultural Biotech- nology Research Institute of Iran (ABRII), Karaj, Iran
American Journal of Plant Sciences·Volume 05 (2014)·Pages 409–415·Published 26 January 2014·DOI10.4236/ajps.2014.53054
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Abstract
Thirteen microsatellite markers were isolated and characterized from Juglans regia L . Successful polymorphism of the 13 primer pairs was observed in various genotypes of J. regia . The number of polymorphic alleles ranged from 2 to 4 (with an average of 4.35). The polymorphic information content values ranged from 0.47 to 0.88 (with an average of 0.69). TC/AG and GAA/CTT class of repeats were the most abundant di-nucleotide and tri-nucleotide repeats, respectively.
KeywordsIsolationMicrosatellite<i>Juglans regia</i>L.PolymorphismSSR Markers
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