The mitogen-activated protein kinase (MAPK) cascade is the most important mechanism in environmental responses and developmental processes in plants. The OsMAPK 2 gene has been found to function in plant tolerance to diverse biotic/abiotic stresses. This paper presents evidence that OsMAPK 2 ( Oryza sativa MAP kinase gene 2) is responsive to P i deficiency and involved in P i homeostasis. We found that full-length expression of OsMAPK 2 was up-regulated in both rice plants and cell culture in the absence of inorganic phosphate (P i ). The transgenic rice and Arabidopsis plants overexpressing OsMAPK 2 showed affected root development and increased plant P i content compared with wild-type plants. Overexpression of OsMAPK 2 controlled the expression of several P i starvation-responsive genes. Our results indicated that OsMAPK 2 enables tolerance phosphate deficiency and is involved in P i homeostasis.
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