Evaluation of Phenolic Content and Antioxidant Property of <i>Crossandra infundibuliformis</i> Leaves Extracts

Abstract

The present study was aimed to investigate the phenolic content and antioxidant property of Crossandra infundibuliformis leaves. Shade dried leaves were successively extracted and determined phenolic and flavonol content of the extracts . Six different in vitro antioxidant assays such as 1, 1’-diphenyl-2-picrylhydrazyl (DPPH), lipid peroxidation (LPO), nitric oxide, hydrogen peroxide radical scavenging, reducing power of the extracts and total antioxidant capacity (values were expressed as mg ascorbic acid (vitamin C) equivalent antioxidant capacity per gram of extracts) assays were followed to determine the antioxidant capacity of the extracts. The results of this study indicated that methanol extract possess higher level of phenolic (98.52 mg of gallic acid/g of extract) and flavonol (84.59 mg of rutin/g of extract) constituents. The antioxidant property of ethyl acetate extract was significantly higher than other extracts in DPPH method. Moreover, methanol extract has significantly higher antioxidant activity in LPO inhibition (IC 50 value of 70.66 μg/ml), hydrogen peroxide (IC 50 value 130.33 μg/ml) and reducing capacity of the extract (1.19) than other chloroform and ethyl acetate extracts. The strong correlation was observed for phenolic content and antioxidant activities of the extracts ensuring the involvement of phenols in antioxidant activity. However, results suggests that Crossandra infundibuliformis leaves possess antioxidant activity.