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<i>In Vitro</i> Induction of Polyploidy in <i>Citrus reticulata</i> Blanco
Horticulture Department, Faculty of Agriculture, Kafrelsheikh University, Kafr El-Sheikh, Egypt
Horticulture Department, Faculty of Agriculture, Kafrelsheikh University, Kafr El-Sheikh, Egypt
- 1 Horticulture Department, Faculty of Agriculture, Kafrelsheikh University, Kafr El-Sheikh, Egypt
- 2 Horticulture Department, Faculty of Agriculture, Kafrelsheikh University, Kafr El-Sheikh, Egypt
American Journal of Plant Sciences·Volume 05 (2014)·Pages 1679–1685·Published 14 May 2014·DOI10.4236/ajps.2014.511182
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Abstract
Possibility of polyploidy induction by colchicine in “Balady” mandarins was investigated in vitro . Seeds were immersed in different concentrations of colchicine solutions (0.01%, 0.05%, 0.1% and 0.2%) for different durations (12, 24 and 48 hr), then cultured in vitro on MS medium at half strength. Seed survival percentage decreased by increasing colchicine concentration and duration of treatment showing the lowest percentage at 0.2% for 48 hr. The highest DNA content was recorded at 0.2% for 24 hr. Stomata No. per unit area was decreased by colchicine treatments; more over stomata length and width were studied. The results indicated that colchicine treatment at 0.1% for 48 hr had the highest tetraploid induction efficiency percentage.
KeywordsColchicineDNAMandarinPolyploidyTetraploid
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