Increased plant tolerance to stress may be chemically induced with applications of salicylic acid (SA). The aim of this study was to determine the change in the SA leaf concentration over time in response to the SA spraying in leaves of greenhouse grown tomato. In sprayed leaves the SA concentration showed changes over time similar to the reported responses to environmental stress. Two days after the first application, the SA foliar concentration reached the maximum of 8 μg·g -1 , equivalent to twice the amount observed in the control plants. SA decreased until it reached the level of control plants eight days later. A second application showed actually the same response, but with a faster decline of SA in two days. According to the results of this assay, SA applications on tomato should be performed within a minimum interval of eight days in order to maintain the SA concentration related with the increase in plant tolerance to environmental stress.
Browse, J. (2009) Jasmonate Passes Muster: A Receptor and Targets for the Defense Hormone. Annual Review of Plant Biology, 60, 183-205. http://dx.doi.org/10.1146/annurev.arplant.043008.092007
Ashraf, M., Akram, N.A., Arteca R.N. and Foolad, M.R. (2010) The Physiological, Biochemical and Molecular Roles of Brassinosteroids and Salicylic Acid in Plant Processes and Salt Tolerance. Critical Reviews in Plant Sciences, 29, 162-190. http://dx.doi.org/10.1080/07352689.2010.483580
Noreen, S., Ashraf, M., Hussain, M. and Jamil, A. (2009) Exogenous Application of Salicylic Acid Enhances Antioxidative Capacity in Salt Stressed Sunflower (Helianthus annus L.) Plants. Pakistan Journal of Botany, 41, 473-479.
Khan, N.A., Syeed, S., Masood, N., Nazar, R. and Iqbal, N. (2010) Application of Salicylic Acid Increases Contents of Nutrients and Antioxidative Metabolism in Mungbean and Alleviates Adverse Effects of Salinity Stress. International Journal of Plant Biology, 1, e1. http://web.b.ebscohost.com/ehost/pdfviewer/pdfviewer?sid=2f426a81-8cf2-4521-9fbe-9a207ab70a10%40sessionmgr1 12&vid=1&hid=127
Purcarea, C. and Cachita-Cosma, D. (2010) Studies Regarding the Effects of Salicylic Acid in Maize (Zea mays L.) Seedling under Salt Stress. Studia Universitatis “Vasile Goldis”, Seria Stiintele Vietii, 20, 63-68.
Girling, R.D., Madison, R., Hassall, M., Poppy, G.M. and Turner, J.G. (2008) Investigation into Plant Bi-ochemical Wound-Responses Pathways Involved in the Production of Aphid-Induced Plant Volatiles. Journal of Expe-rimental Botany, 59, 3077-3085. http://dx.doi.org/10.1093/jxb/ern163
Zhang, Z., Li, Z., Staswick, E., Wang, M., Zhu, Y. and He, Z. (2009) Dual Regulation Role of GH3.5 in Salicylic Acid and Auxin during Arabidop-sis-Pseudomonas siringae Interaction. Plant Physiology, 145, 450-464. http://dx.doi.org/10.1104/pp.107.106021
Ogawa, T., Ara, T., Aoki, K., Suzuki, H. and Shibata, D. (2010) Transient Increase in Salicylic Acid and Its Glucose Conjugates after Wounding in Arabidopsis Leaves. Plant Biotechnology, 27, 205-209. http://dx.doi.org/10.5511/plantbiotechnology.27.205
Benavides, A., Salazar, A., Ramírez, F., Robledo, V., Ramírez, H. and Maiti, R. (2004) Tratamiento de semilla de chile con ácido salicílico y sulfosalicílico y respuesta de las plántulas al frío. Terra Latinoamericana, 22, 41-47.
Noreen, S. and Ashraf, M. (2008) Alleviation of Adverse Effects of Salt Stress on Sunflower (Helianthus annuus L.) by Exogenous Application of Salicylic Acid: Growth and Photosynthesis. Pakistan Journal of Botany, 40, 1657-1663.
Ahmad, I., Basra, S.M.A., Afzal, I., Farooq, M. and Wahid, A. (2013) Growth Improvement in Spring Maize through Exogenous Application of Ascorbic Acid, Salicylic Acid and Hydrogen Peroxide. International Journal of Agriculture and Biology, 15, 95-100.
Khodary, S. (2004) Effect of Salicylic Acid on Growth, Photosynthesis and Carbohydrate Metabolism in Salt-Stressed Maize Plants. International Journal of Agriculture and Biology, 6, 5-8.
Gemes, K., Poor, P., Sulyok, Z., Szepesi, A., Szabo, M. and Tari, I. (2008) Role of Salicylic Acid Pre-Treatment on the Photosynthetic Performance of Tomato Plants (Lycopersicum sculentum Mill. L. cv. Rio Fuego) under Salt Stress. Acta Biologica Szegediensis, 52, 161-162.
Azooz, M.M. (2009) Salt Stress Mitigation by Seed Priming with Salicylic Acid in Two Faba Bean Genotypes Differing in Salt Tolerance. International Journal of Agriculture and Biology, 11, 343-350.
Noreen, S. and Ashraf, M. (2009) Assessment of Variation in Antioxidative Defense System in Salt-Treated Pea (Pisum sativum) Cultivars and Its Putative Use as Salinity Tolerant Markers. Journal of Plant Physiology, 166, 1764-1774. http://dx.doi.org/10.1016/j.jplph.2009.05.005
El-Khallal, S.M., Hathout, A., Ahsour, A. and Kerrit, A. (2009) Brassinolide and Salicylic Acid Induced Antioxidant Enzymes, Hormonal Balance and Protein Profile of Maize Plants Grown under Salt Stress. Research Journal of Agriculture and Biological Sciences, 5, 391-402.
Jabbarzadeh, J., Khosh, K.M. and Salehi, H. (2009) The Effect of Foliar Applied Salicylic Acid on Flowering of African Violet. Australian Journal of Basic and Applied Sciences, 3, 4693-4696.
Najafian, S., Khoshkhui, M., Tavallali, V. and Saharkhiz, M.J. (2009) Effect of Salicylic Acid and Salinity in Thyme (Thymus vulgaris L.): Investigation on Changes in Gas Exchange, Water Relations, and Membrane Stabilization and Biomass Accumulation. Australian Journal of Basic and Applied Sciences, 3, 2620-2626.
Villanueva, E., Alcantar, G., Sánchez, P., Soria, M. and Larque, A. (2009) Efecto del ácido salicílico y dimetilsulfoxido en floración de Crisantemum morifolium (Ramat) Kitamura en Yucatán. Revista Chapingo, Serie Horticultura, 15, 25-31.
Azooz, M. and Youseef, M. (2010) Evaluation of Heat Shock and Salicylic Acid Treatments as Inducers of Drought Stress Tolerance in Wheat. American Journal of Plant Physiology, 5, 56-70. http://dx.doi.org/10.3923/ajpp.2010.56.70
Esmailzadeth, M., Soleimani, M.J. and Rouhani, H. (2008) Exogenous Application of Salicylic Acid for Induced Systemic Acquired Resistance against Tomato Steam Canker Disease. Journal of Biological Sciences, 8, 1039-1044. http://dx.doi.org/10.3923/jbs.2008.1039.1044
Norman, C., Howell, K.A., Millar, A.H., Whelan, J.M. and Day, D.A. (2004) Salicylic Acid Is an Uncoupler and Inhibitor of Mitochondrial Electron Transport. Plant Physiology, 134, 492-501. http://dx.doi.org/10.1104/pp.103.031039
Kaydan, D., Yagmur, M. and Okut, N. (2006) Effects of Salicylic Acid on the Growth and Some Physiological Characters in Salt Stressed Wheat (Triticum aestivum L.). Tarim Bilimleri Dergisi, 13, 114-119.
Khalili, M., Hasanloo, T., Kasemi, T.S.K. and Sepehrifar, R. (2010) Effect of Salicylic Acid on Antioxidant Activity in Milk Thistle Haired Root Cultures. Journal of Medicinal Plants, 35, 51-61.
Steiner, A.A. (1961) A Universal Method for Preparing Nutrient Solutions of a Certain Desired Composition. Plant and Soil, 15, 134-154. http://dx.doi.org/10.1007/BF01347224
Lian, B., Zhou, X., Miransari, M. and Smith, D.L. (2000) Effects of Salicylic Acid on the Development and Root Nodulation of Soybean Seedling. Journal of Agronomy and Crop Science, 185, 187-192. http://dx.doi.org/10.1046/j.1439-037x.2000.00419.x
He, W., Li, H., Li, X., Li, M.Q. and Chen, Y.W. (2007) Tetranynchus urticae Koch Induced Accumulation of Salicylic Acid in Frijole Leaves. Pesticide Biochemistry and Physiology, 88, 78-81. http://dx.doi.org/10.1016/j.pestbp.2006.09.002
Kojima, H., Hossain, M.M., Kubota, M. and Hyakumachi, M. (2013) Involvement of the Salicylic Acid Signaling Pathway in the Systemic Resistance Induced in Arabidopsis by Plant Growth-Promoting Fungus Fusarium equiseti GF19-1. Journal of Oleo Science, 62, 415-426. http://dx.doi.org/10.5650/jos.62.415
Molders, W., Buchala, A. and Metraux, J.P. (1996) Transport of Sali-cylic Acid in Tobacco Necrosis Virus Infected Cucumber Plants. Plant Physiology, 112, 787-792.
Enyedi, A.J., Nalpai, N., Silverman, P. and Raskin, I. (1992) Localization, Conjugation, and Function of Salicylic Acid on Tobacco during Hypersensitive Reaction to Tobacco Mosaic Virus. Proceedings of the National Academy of Sciences of the United States of America, 89, 2480-2484. http://dx.doi.org/10.1073/pnas.89.6.2480
Shulaev, V., León, J. and Raskin, I. (1995) Is Salicylic Acid a Translocated Signal of Systemic Acquired Resistance in Tobacco? The Plant Cell, 7, 1691-1701. http://dx.doi.org/10.1105/tpc.7.10.1691
Seskar, M., Shulaev, V. and Raskin, I. (1998) Endogenous Methyl Salicylate in Pathogen-Inoculated Tobacco Plants. Plant Physiology, 116, 387-392. http://dx.doi.org/10.1104/pp.116.1.387
Park, S.W., Kaimoyo, E., Kumar, D., Mosher, S. and Klessig, D.F. (2007) Methyl Salicylate Is a Critical Mobile Signal for Plant Systemic Acquired Resistance. Science, 318, 113-116. http://dx.doi.org/10.1126/science.1147113
Prithiviraj, B., Bais, H.P., Weir, T., Suresh, B., Najarro, E.H., Dayacar, B.V., Swheizer, H.P. and Vivanco, J.M. (2005) Down Regulation of Virulence Factors of Pseudomonas ae-ruginosa by Salicylic Acid Attenuates Its Virulence on Arabidopsis thaliana and Caenobhabditis elegans. Infection and Immunity, 73, 5319-5328. http://dx.doi.org/10.1128/IAI.73.9.5319-5328.2005
Mateo, A., Funck, D., Mühlenbock, P., Kular, B., Mullineaux, P.M. and Karpinski, S. (2006) Controlled Levels of Salicylic Acid Are Re-quired for Optimal Photosynthesis and Redox Homeostasis. Journal of Experimental Botany, 57, 1795-1807. http://dx.doi.org/10.1093/jxb/erj196
Genoud, T., Bachala, A.J., Chua, N.H. and Metraux, J.P. (2002) Phy-tochrome Signaling Modulates the SA-Perceptive Pathway in Arabidopsis. The Plant Journal, 31, 87-95. http://dx.doi.org/10.1046/j.1365-313X.2002.01338.x
Klämbt, H.D. (1962) Conversion in Plants of Benzoic Acid to Salicylic Acid and Its βd-Glucoside. Nature, 196, 491. http://dx.doi.org/10.1038/196491a0
Yalpani, N., Silverman, P., Wilson, A., Kleier, D. and Raskin, I. (1991) Salicylic Acid Is a Systemic Signal and an Inducer of Pathogenesis Related Protein in Virus Infected Tobacco. The Plant Cell, 3, 809-818. http://dx.doi.org/10.1105/tpc.3.8.809
Wildermut, M.C., Dewdney, J., Wu, G. and Ausubel, F.M. (2001) Isochorismathe Synthase Is Required to Synthesize Salicylic Acid for Plant Defence. Nature, 414, 562-565. http://dx.doi.org/10.1038/35107108
Mustafa, N.R., Kin, H.K., Choi, Y.H. and Vepoorte, R. (2009) Metabolic Changes of Salicylic Acid-Elicited Catharanthus roseus Cell Suspension Cultures Monitored by NMR-Based Metabolomics. Biotechnology Letters, 31, 1967-1974. http://dx.doi.org/10.1007/s10529-009-0107-1
Panina, Y.S., Gerasimova, N.G., Chalenco, G.I., Vasyukova, N.I. and Ozeretskovskaya, O.L. (2005) Salicylic Acid and Phe-nylalanine Ammonia-Lyase in Potato Plants Infected with the Causal Agent of Late Blight. Russian Journal of Plant Physiology, 52, 511-515. http://dx.doi.org/10.1007/s11183-005-0075-9
Tao, Y., Xie, Z., Chen, W., Glaze-brook, J., Chang, H.S., Han, R., Zhu, T., Zou, G. and Katagiri, F. (2003) Quantitative Nature of Arabidopsis Responses during Compatible and Incompatible Interactions with the Bacterial Pathogen Pseudomonas syringae. The Plant Cell, 15, 317-330. http://dx.doi.org/10.1105/tpc.007591
Abreu, M.E. and Munne, B.S. (2009) Salicylic Acid De-ficiency NahG Transgenic Lines and sid2 Mutants Increases Seed Yield in the Annual Plant Arabidopsis thaliana. Journal of Experimental Botany, 60, 1261-1271. http://dx.doi.org/10.1093/jxb/ern363
Malamy, J., Henning, J. and Klessig, D.F. (1992) Temperature-Dependent Induction of Salicylic Acid and Its Conjugates during the Resistance Response to Tobacco Mosaic Virus Infection. The Plant Cell, 4, 359-366. http://dx.doi.org/10.1105/tpc.4.3.359
Barbaus, R.L. and Jacobsen, B.J. (2007) Biocontrol Elicited Systemic Resistance in Sugarbeet Is Salicylic Acid Independent and NPR1 Dependent. Journal of Sugar Beet Research, 44, 17-35. http://dx.doi.org/10.5274/jsbr.44.1.17
Iwai, T., Seo, S., Mitsuhara, I. and Ohashi, K. (2007) Probenazole Induced Accumulation of Salicylic Acid Confers Resistance to Magnaporte griseain Adults Plants Rice. Plant and Cell Physiology, 48, 915-924. http://dx.doi.org/10.1093/pcp/pcm062
Wang, L.J., Fan, L., Loescher, W., Duan, G.J., Cheng, J.S., Luo, H.B. and Li, S.H. (2010) Salicylic Acid Alleviates Decreases in Photosynthesis under Heat Stress and Accelerates Recovery in Grapevine Leaves. BMC Plant Biology, 10, 34.
Tuna, L., Kaya, C., Dikilitas, M., Yokas, I., Burum, B. and Altulnu, H. (2007) Comparative Effect of Various Salicylic Acid Derivates on Key Growth Parameters and Some Enzyme Activities in Salinity Stressed Maize (Zea mays L.) Plants. Pakistan Journal of Botany, 39, 787-798.
Umebese, C.T., Olatimilehin, T.O. and Ogunsusi, T.A. (2009) Salicylic Acid Protects Nitrate Reductase Activity, Growth and Proline in Amaranth and Tomato Plants during Water Deficit. American Journal of Agricultural and Biological Sciences, 4, 224-229. http://dx.doi.org/10.3844/ajabssp.2009.224.229