Somatic Embryogenesis and Organogenesis for Regeneration of Endangered Multipurpose Desert Plant <i>Leptadenia pyrotechnica</i> Forsk. Decne in the Kingdom of Bahrain — Oak Academic Publishing
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Somatic Embryogenesis and Organogenesis for Regeneration of Endangered Multipurpose Desert Plant <i>Leptadenia pyrotechnica</i> Forsk. Decne in the Kingdom of Bahrain
Plant Tissue Culture Laboratory, Desert and Arid Zone Sciences Program, College of Graduate Studies, Arabian Gulf University, Manama, Kingdom of Bahrain
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Plant Tissue Culture Laboratory, Desert and Arid Zone Sciences Program, College of Graduate Studies, Arabian Gulf University, Manama, Kingdom of Bahrain
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Plant Tissue Culture Laboratory, Desert and Arid Zone Sciences Program, College of Graduate Studies, Arabian Gulf University, Manama, Kingdom of Bahrain
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Plant Tissue Culture Laboratory, Desert and Arid Zone Sciences Program, College of Graduate Studies, Arabian Gulf University, Manama, Kingdom of Bahrain
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Plant Tissue Culture Laboratory, Desert and Arid Zone Sciences Program, College of Graduate Studies, Arabian Gulf University, Manama, Kingdom of Bahrain
1 Plant Tissue Culture Laboratory, Desert and Arid Zone Sciences Program, College of Graduate Studies, Arabian Gulf University, Manama, Kingdom of Bahrain
2 Plant Tissue Culture Laboratory, Desert and Arid Zone Sciences Program, College of Graduate Studies, Arabian Gulf University, Manama, Kingdom of Bahrain
3 Plant Tissue Culture Laboratory, Desert and Arid Zone Sciences Program, College of Graduate Studies, Arabian Gulf University, Manama, Kingdom of Bahrain
4 Plant Tissue Culture Laboratory, Desert and Arid Zone Sciences Program, College of Graduate Studies, Arabian Gulf University, Manama, Kingdom of Bahrain
5 Plant Tissue Culture Laboratory, Desert and Arid Zone Sciences Program, College of Graduate Studies, Arabian Gulf University, Manama, Kingdom of Bahrain
Leptadenia pyrotechnica is an important multipurpose endangered plant in the Kingdom of Bahrain with restricted distribution. Nodal explants were cultured on Murashige and Skoog (MS) medium supplemented with different concentrations and combinations of indole acetic acid (IAA) and 6-benzylaminopurine (BAP). Initially, 80% and 60% explants responded in direct shoot and callus initiation response respectively in presence of 8.88 μM BAP with 5.71 μM IAA in modified MS media after two weeks of culture. The highest frequency of plant regeneration was observed in presence of 8.88 μM BAP with 1.14 μM IAA following organogenic pathway of differentiation. Hundred percent callus proliferation was observed while initial callus developed in presence of 4.44 μM BAP with 2.85 μM IAA and was transferred in media containing 4.44 μM, 6.66 μM BAP with 2.85 μM IAA and 13.32 μM BAP with 5.71 μM IAA. The callus derived plants were regenerated following the pathway of indirect somatic embryogenesis. The induction of somatic embryogenesis and plant regeneration from callus was also observed in modified MS media supplemented with 4.44 μM BAP and 2.85 μM IAA. The plant regeneration protocol we developed for Leptadenia pyrotechnica will be very beneficial for biodiversity conservation and environment protection of Bahrain. Moreover, the present paper reports for the first time specifically the somatic embryogenesis in this multipurpose desert plant Leptadenia pyrotechnica.
El-Oqlah, A. and Abbas, J. (1994) A Checklist of Vascular Plants of Bahrain. Dirasat Journal, 21, 95-118.
Jameel, A., Al-Laith, A. and Qaher, M. (2010) Knowledge, Attitudes, and Practices Regarding Medicinal Plants Used by the Indigenous People of Bahrain. Arab Gulf Journal of Scientific Research, 28, 105-117.
Bennett, R.N. and Wallsgrove, R.M. (1994) Secondary Metabolites in Plant Defense Mechanisms. New Phytology, 127, 617-633. http://dx.doi.org/10.1111/j.1469-8137.1994.tb02968.x
Grayer, R.J. and Harborne, J.J. (1994) A Survey of Antifungal Compounds from Higher Plants, 1982-1993. Phytochemistry, 37, 19-42. http://dx.doi.org/10.1016/0031-9422(94)85005-4
Al-Eisawi, D. (2003) Effect of Biodiversity Conservation on Arid Ecosystem with a Special Emphasis on Bahrain. Journal of Arid Environments, 54, 81-90. http://dx.doi.org/10.1006/jare.2001.0888
Khanna, A. (2012) Implementation of UNCCD in the GCC Countries. In: Khanna, A., Ed., Global Environmental Governance and Desertification: A Study of Gulf Corporation Council Countries. Concept Publishing Company Pvt. Ltd., New Delhi, 272.
Dagla, H.R., Paliwal, A., Rathore, M.S. and Shekhawat, N.S. (2012) Micropropagation of Leptadenia pyrotechnica (Forsk.) Decne: A Multipurpose Plant of an Arid Environment. Journal of Sustainable Forestry, 31, 283-293. http://dx.doi.org/10.1080/10549811.2011.582831
Khasawneh, A.M., Elwy, M.H., Hamza, A.A., Fawzi, M.N. and Hassan, H.A. (2011) Antioxidant Anti-Lipoxygenase and Cytotoxic Activity of Leptadenia pyrotechnica (Forssk.) Decne Polyphenolic Constituents. Molecules, 16, 7510-7521. http://dx.doi.org/10.3390/molecules16097510
Aquino, R., Peluso, G., Tommasi, N.D., Simone, F.D. and Pizza, C. (1996) New Polyoxypregnane Ester Derivatives from Leptadenia hastate. Journal of Natural Product, 59, 555-564. http://dx.doi.org/10.1021/np960251e
Abd El-Ghani, M.M. and Amer, W.M. (2003) Soil-Vegetation Relationships in a Coastal Desert Plain of Southern Sinai, Egypt. Journal of Arid Environments, 55, 607-628. http://dx.doi.org/10.1016/S0140-1963(02)00318-X
Cioffi, G., Sanogo, R., Vassallo, A., Dal Piaz, F., Autore, G., Marzocco, S. and Tommasi, N.D. (2006) Pregnane Glycosides from Leptadenia pyrotechnica. Journal of Natural Product, 69, 625-635. http://dx.doi.org/10.1021/np050493r
Panwara, J. and Tarafdarb, J.C. (2006) Distribution of Three Endangered Medicinal Plant Species and Their Colonization with Arbuscular Mycorrhizal Fungi. Journal of Arid Environments, 65, 337-350. http://dx.doi.org/10.1016/j.jaridenv.2005.07.008
Jain, G.C., Jhalani, J.C., Agarwal, S. and Jain, K. (2007) Hypolipidemic and Antiatherosclerotic Effect of Leptadenia pyrotechnica Extracts in Cholestrol Fed Rabbits. Asian Journal of Experimental Science, 21, 115-122.
Munazir, M., Qureshi, R., Arshad, M. and Gulfraz, M. (2012) Antibacterial Activity of Root and Fruit Extracts of Leptadenia pyrotechnica (Asclepiadaceae) from Pakistan. Pakistan Journal of Botany, 44, 1209-1213.
Paunescu, A. (2009) Biotechnology for Endangered Plant Conservation: A Critical Overview. Romanian Biotechnological Letters, 14, 4095-4103.
Khan, S., Al-Qurainy, F. and Mohammad, N. (2012) Biotechnological Approaches for Conservation and Improvement of Rare and Endangered Plants of Saudi Arabia. Saudi Journal of Biological Sciences, 19, 1-11.
Von Arnold, S., Sabala, I., Bozhkov, P., Dyachok, J. and Filonova, L. (2002) Developmental Pathways of Somatic Embryogenesis. Plant Cell, Tissue and Organ Culture, 69, 233-249. http://dx.doi.org/10.1023/A:1015673200621
Yang, X.Y. and Zhang, X.L. (2010) Regulation of Somatic Embryogenesis in Higher Plants. Critical Reviews in Plant Sciences, 29, 36-57. http://dx.doi.org/10.1080/07352680903436291
Pathak, M.R. and Hamzah, R.Y. (2008) An Effective Method of Sonication-Assisted Agrobacterium-Mediated Transformation of Chickpea. Plant Cell, Tissue and Organ Culture, 93, 65-71. http://dx.doi.org/10.1007/s11240-008-9344-6
Ray, M., Ghosh, S. and Ghosh, B. (1996) Plant Regeneration from Embryogenic Calli of Heat Tolerant and Sensitive Cultivars under Thermal Stress. Physiology and Molecular Biology of Plant, 2, 59-66.
Benková, E., Michniewicz, M., Sauer, M., Teichmann, T., Seifertová, D., Jürgens, G. and Friml, J. (2003) Local, EffluxDependent Auxin Gradients a Common Module for Plant Organ Formation. Cell, 115, 591-602. http://dx.doi.org/10.1016/S0092-8674(03)00924-3
Pernisová, M., Klíma, P., Horák, J., Válková, M., Malbeck, J., Soucek, S., Reichman, P., Hoyerová, K., Dubová, J., Jirí, F., Eva, Z. and Jan, H. (2009) Cytokinins Modulate Auxin-Induced Organogenesis in Plants via Regulation of the Auxin Efflux. Proceedings of the National Academy of Sciences of the United States of America, 106, 3609-3614. http://dx.doi.org/10.1073/pnas.0811539106
Dagla, H.R., Paliwal, A., Rathore, M.S. and Shekhawat, N.S. (2012) Micropropagation of Leptadenia pyrotechnica (Forsk.) Decne: A Multipurpose Plant of an Arid Environment. Journal of Sustainable Forestry, 31, 283-293. http://dx.doi.org/10.1080/10549811.2011.582831
Murashige, T. and Skoog, F. (1962) A Revised Medium for Rapid Growth and Bioassay with Tobacco Tissue Culture. Physiologia Plantarum, 15, 473-497. http://dx.doi.org/10.1111/j.1399-3054.1962.tb08052.x
Gamborg, O., Miller, R. and Ojimi, K. (1968) Nutrient Requirements of Suspension Cultures of Soybean Root Cells. Experimental Cell Research, 50, 151-158. http://dx.doi.org/10.1016/0014-4827(68)90403-5
Pathak, M.R. and Hamzah, R.Y. (2010) In Vitro Plant Regeneration and Genetic Transformation of Chickpea (Cicer arietinum L). In: Sivakumar, P., Gnanam, R., Khawar, K.M. and Thangadurai, D., Eds., Grain Legume Research: Tissue Culture, Biotechnology and Genetic Engineering, 2, Bio Science Publications, Tamil Nadu, 11-19.
Chen, C.C., Chen, S.J., Sagare, A.P. and Tsay, H.S. (2001) Adventitious Shoot Regeneration from Stem Internode Explants of Adenophora triphylla (Thunb) A.C. (Campanulaceae)—An Important Medicinal Herb. Botanical Bulletin of Academia Sinica, 42, 1-7.
Turker, A.U., Yucesan, B. and Gurel, E. (2010) Adventitious Shoot Regeneration from Stem Internode Explants of Verbena officinalis L., a Medicinal Plant. Turkish Journal of Biology, 34, 297-304.
Sangavai, C. and Chellapandi, P. (2008) In Vitro Propagation of a Tuberose Plant (Polianthes tuberose L.). Electronic Journal of Biology, 4, 98-101.
Sharon, M., Marie, C.C. and Sharon, M. (2012) In Vitro Culture Studies of Bixa orellana L: III. Plant Regeneration from Roots through Direct and Indirect Somatic Embryogenesis. European Journal of Experimental Biology, 2, 156-162.
Bohidar, S., Thriunavoukkarasu, M. and Reo, V. (2008) Effect of Growth Regulators on in Vitro Micropropagation of “Garden Rue” Ruta graveolens L. International Journal of Integrated Biology, 3, 36-43.
Mohebalipour, N., Aharizad, S., Mohammadi, S., Motallebiazar, A. and Arefi, H. (2012) Effect of Plant Growth Regulators BAP and IAA on Micropropagation of Iranian Lemon Balm (Melissa officinalis L.) Landraces. Journal of Food, Agriculture and Environment, 10, 280-286.
Makunga, N. and Staden, J. (2008) An Efficient System for the Production of Clonal Plantlets of the Medicinally Important Aromatic Plant: Salvia Africana-lutea L. Plant Cell, Tissue and Organ Culture, 92, 63-72. http://dx.doi.org/10.1007/s11240-007-9305-5
Sen, A., Sharma, M.M., Grover, D. and Batra, A. (2009) In Vitro Regeneration of Phyllanthus amarus Schun. and Thonn.: An Important Medicinal Plant. Our Nature, 7, 110-115.
Da Silva, S., Sato, A., Luiz, C., Lage, S., Azevedo, A. and Apparecida, E.M. (2005) Essential Oil Composition of Melissa officinalis L. in Vitro Produced under the Influence of Growth Regulators. Journal of the Brazilian Chemical Society, 16, 1387-1390. http://dx.doi.org/10.1590/S0103-50532005000800014
Ghiorghita, G.I., Mafteli, D.E.S. and Nicuta, D.N. (2005) Investigations on the in Vitro Morphgenetic Reaction of Melissa officinalis L. Species. Genetics and Molecular Biology, 5, 119-125.
Munazir, M., Qureshi, R., Ali, M., Rashid, U., Noor, S., Mehmood, K., Ali, S. and Arshad, M. (2010) Primary Callus Induction, Somatic Embryogenesis and Regeneration Studies in Elite Wheat Varieties from Pakistan. Pakistan Journal of Botany, 42, 3957-3965.
Coenen, C. and Lomax, T.L. (1997) Auxin-Cytokinin Interactions in Higher Plants: Old Problems and New Tools. Trends in Plant Science, 2, 351-356. http://dx.doi.org/10.1016/S1360-1385(97)84623-7
Shekhawat, N.S., Kackar, A., Rathore, M.S., Singh, M., Dagla, H.R. and Vinod, A. (2006) Establishment and Economic Evaluation of Micropropagated Jeewanti (Leptadenia reticulate Wight & Arn.) Plants in Field. Natural Product Radiance, 5, 311-314.
Dubrovsky, J.G., Sauer, M., Napsucialy-Mendivil, S., Ivanchenko, M.G., Friml, J., Shishkova, S., Celenza, J. and Benková, E. (2008) Auxin Acts as a Local Morphogenetic Trigger to Specify Lateral Root Founder Cells. Proceedings of the National Academy of Sciences of the United States of America, 105, 8790-8794. http://dx.doi.org/10.1073/pnas.0712307105
Gokhale, M. and Bansal, Y.K. (2009) Direct in Vitro Regeneration of a Medicinal Tree Oroxylum indicum (L.) Vent. through Tissue Culture. African Journal of Biotechnology, 8, 3777-3781.
Sadeq, M.A., Pathak, M.R., Ahmed, A.S., Abido, M. and Abahussain, A. (2014) Highly Efficient in Vitro Regeneration Method of Endangered Medicinal Plant Heliotropium kotschyi (Ramram) in the Kingdom of Bahrain. Ameriacn Journal of Plant Sciences, 5, 736-747. http://dx.doi.org/10.4236/ajps.2014.55088
Varshney, V., Kant, T., Sharma, V.K., Rao, A. and Kothari, S.L. (1996) High Frequency Plant Regeneration from Immature Embryo Cultures of Triticum aestivum and T. durum. Cereal Research Communication, 24, 409-416.
Radojevic, L. and Subotic, A. (1992) Plant Regeneration of Iris setosa Pall. Through Somatic Embryogenesis and Organogenesis. Journal of Plant Physiology, 139, 690-696. http://dx.doi.org/10.1016/S0176-1617(11)81712-8
Roy, A. and Bhattacharya, S. (2008) An Improved Micropropagation of Eclipta alba by in Vitro Priming with Chlorocholine Chloride. Plant Cell, Tissue and Organ Culture, 92, 315-319. http://dx.doi.org/10.1007/s11240-007-9328-y
Saini, R., Sharma, A., Sharma, M.M. and Batra, A. (2011) Impacts of Phytohormones in Micropropagation of Medicinally Potent Plant: Boerhavia diffusa L. International Journal of Pharmaceutical Sciences Review and Research, 8, 85-89.
Ujjwala, J.S. (2007) In Vitro Regeneration of Aloe barbadensis. Biotechnology, 6, 601-603. http://dx.doi.org/10.3923/biotech.2007.601.603
Komamine, A., Murata, N. and Nomura, K. (2005) Mechanisms of Somatic Embryogenesis in Carrot Suspension Cultures—Morphology, Physiology, Biochemistry and Molecular Biology. In Vitro Cellular & Developmental Biology-Plant, 41, 6-10. http://dx.doi.org/10.1079/IVP2004593
Schmidt, E., Guzzo, D.F., Toonen, M.M. and De Vries, S.C. (1997) A Leucine-Rich Repeat Containing Receptor-Like Kinase Marks Somatic Plant Cells Competent to Form Embryos. Development, 124, 2049-2062.
Ikeda-Iwai, M., Satoh, S. and Kamada, H. (2002) Establishment of a Reproducible Tissue Culture System for the Induction of Arabidopsis Somatic Embryos. Journal of Experimental Botany, 53, 1575-1580. http://dx.doi.org/10.1093/jxb/erf006
Quiroz-Figueroa, F.R., Fuentes-Cerda, C.F.J., Rojas-Herrera, R. and Loyola-Vargas, V.M. (2002) Histological Studies on the Developmental Stages and Differentiation of Two Different Somatic Embryogenesis Systems of Coffea arabica. Plant Cell Report, 20, 1141-1149. http://dx.doi.org/10.1007/s00299-002-0464-x
Singh, P., Singh, A., Shukla, A., Singh, L., Pande, V. and Nailwal, T. (2009) Somatic Embryogenesis and in Vitro Regeneration of an Endangered Medicinal Plant Sarpgandha (Rauvolfia serpentina. L). Life Science Journal, 6, 74-79.
Mujib, A., Banerjee, S. and Ghosh, P.D. (2005) Origin, Development and Structure of Somatic Embryos in Selected Bulbous Ornamentals: Bap as Inducer. In: Mujib, A. and Samaj, J., Eds., Plant Cell Monograph. Somatic Embryogenesis, Springer-Verlag, Berlin, 15-24.
Sahrawat, A.K. and Chand, S. (2001) Continuous Somatic Embryogenesis and Plant Regeneration from Hypocotyl Segments of Psoralea corylifolia Linn., an Endangered and Medicinally Important Fabaceae Plant. Current Science, 81, 25-27.
Choi, Y.E., Kim, J.W. and Yoon, E.S. (1999) High Frequency of Plant Production via Somatic Embryogenesis from Callus or Cell Suspension Cultures in Eleutherococcus senticosus. Annals of Botany, 83, 309-314. http://dx.doi.org/10.1006/anbo.1998.0827
Munoz, T.L. and Garay, R.B. (1996) Somatic Embryogenesis in the Threatened Cactus Turbinicarpus pseudomacrochele (Buxbaum and Backeberg). Journal of the Professional Association for Cactus Development, 1, 36-38.
Verma, M. and Bansal, Y.K. (2012) Induction of Somatic Embryogenesis in Endangered Butterfly Ginger Hedychium coronarium J. Koenig. Indian Journal of Experimental Biology, 50, 904-909.