The Distribution and Morphology Alterations of Microfilaments and Microtubules in Mesophyll Cells and Root-Tip Cells of Wheat Seedlings under Enhanced Ultraviolet-B Radiation — Oak Academic Publishing
Research ArticleOpen AccessGoogle Scholar indexed
The Distribution and Morphology Alterations of Microfilaments and Microtubules in Mesophyll Cells and Root-Tip Cells of Wheat Seedlings under Enhanced Ultraviolet-B Radiation
Higher Education Key Laboratory of Plant Molecular and Environmental Stress Response, Shanxi Normal University, Linfen, China
,
Analysis and Testing Center, Shanxi Normal University, Linfen, China
,
Departrment of Life Science, Lvliang University, Lvliang, China
,
Higher Education Key Laboratory of Plant Molecular and Environmental Stress Response, Shanxi Normal University, Linfen, China
,
Higher Education Key Laboratory of Plant Molecular and Environmental Stress Response, Shanxi Normal University, Linfen, China
1 Higher Education Key Laboratory of Plant Molecular and Environmental Stress Response, Shanxi Normal University, Linfen, China
2 Analysis and Testing Center, Shanxi Normal University, Linfen, China
3 Departrment of Life Science, Lvliang University, Lvliang, China
4 Higher Education Key Laboratory of Plant Molecular and Environmental Stress Response, Shanxi Normal University, Linfen, China
5 Higher Education Key Laboratory of Plant Molecular and Environmental Stress Response, Shanxi Normal University, Linfen, China
The distribution and morphology alterations of microfilaments and microtubules in the mesophyll cells and root-tip cells of wheat seedlings, which had been radiated by enhanced ultraviolet-B (10.08 KJ · m -2 · d -1 ), were examined through the confocal laser scanning microscope (Model FV1000, Olympus, Japan). Microtubule was labeled with an indirect immunofluorescence staining method, and microfilament was labeled with fluorescein isothiocyanate-phalloidin (FITC-Ph) as probes. The results indicated that microtubules in mesophyll cells, compared with the controls, would be depolymerized significantly, and dispersed randomly showing some spots or short rods in the cytoplasm, under the enhanced UV-B radiation condition. The microtubule bundles tended to be diffused, and the fluorescence intensity of that significantly decreased. The distribution pattern of microfilaments, which usually arranged parallelly in control cells, was broken up by enhanced UV-B radiation. We further investigated the distribution and morphology of microtubules in root-tip cells during every stage of cell division, and found that these aberrant phenomena of microtubules were often associated with abnormal cell division. Our findings suggested that the distribution, morphology and structure of cytoskeleton in mesophyll cells and root-tip cells of wheat seedlings would be affected by enhanced UV-B radiation, which might be related to abnormal cell division caused by enhanced UV-B radiation as an extracellular signal.
Rousseaux, C.M., Flint, S.D., Searles, P.S. and Caldwell, M.M. (2004) Plant Responses to Current Solar Ultraviolet-B Radiation and Supplemented Solar Ultraviolet-B Radiation Simulating Ozone Depletion: An Experimental Comparison. Photochemistry and Photobiology, 80, 224-230. http://dx.doi.org/10.1562/2004-03-30-RA-129.1
Allen, D.J., Nogués, S. and Baker, N.R. (1998) Ozone Depletion and Increased UV-B Radiation Is There a Real Threat to Photosynthesis? Journal of Experimental Botany, 328, 1775-1788.
Tanaka, A., Sakamoto, A., Ishigaki, Y., Nikaido, O., Sun, G. and Hase, Y. (2002) An Ultraviolet-B-Resistant Mutant with Enhanced DNA Repair in Arabidopsis. Plant Physiology, 129, 64-71. http://dx.doi.org/10.1104/pp.010894
Fabón, G., Monforte, L., Tomás-Las-Heras, R., Núñez-Olivera, E. and Martínez-Abaigar, J. (2012) Dynamic Response of UV-Absorbing Compounds, Quantum Yield and the Xanthophyll Cycle to Diel Changes in UV-B and Photosynthetic Radiation in an Aquatic Liverwort. Journal of Plant Physiology, 169, 20-26. http://dx.doi.org/10.1016/j.jplph.2011.08.010
Yu, J., Tang, X.X. and Zhang, P.Y. (2005) Physiological and Ultralstructural Changes of Chlorella sp. Induced by UV-B Radiation. Progress of Nature Science, 15, 678-683. http://dx.doi.org/10.1080/10020070512331342750
Mulpuri, V.R., Gopinadhan, P. and Douglas, P.O. (1996) Ultraviolet-B and Ozone-lnduced Biochemical Changes in Antioxidant Enzymes of Arabidopsis thaliana. Plant Physiology, 110, 125-136. http://dx.doi.org/10.1104/pp.110.1.125
Hopkins, L., Bond, M.A. and Tobin, A.K. (2002) Ultraviolet-B Radiation Reduces the Rates of Cell Division and Elongation in the Primary Leaf of Wheat (Triticum aestivum L. cv Maris Huntsman). Plant Cell Environment, 25, 617-624. http://dx.doi.org/10.1046/j.1365-3040.2002.00834.x
Yang, L.Y., Han, R. and Sun, Y. (2013) Effects of Exogenous Nitric Oxide on Wheat Exposed to Enhanced Ultraviolet-B Radiation. American Journal of Plant Sciences, 4, 1285-1290. http://dx.doi.org/10.4236/ajps.2013.46159
Xu, S.X. and Zhu, W. (1996) Plant Cell Cytoskeleton. Science Press, Beijing, 60-83, 94-104. (In Chinese)
Leena, L., Päivi, M.J. and Marikki, L. (2008) Cytoskeleton-Interacting LIM-Domain Protein CRP1 Suppresses Cell Proliferation and Protects from Stress-Induced Cell Death. Experimental Cell Research, 314, 738-747. http://dx.doi.org/10.1016/j.yexcr.2007.11.024
Weber, C., Marchat, A.L., Guillen, N. and López-Camarillo, C. (2009) Effects of DNA Damage Induced by UV Irradiation on Gene Expression in the Protozoan Parasite Entamoeba histolytica. Molecular and Biochemical Parasitology, 164, 165-169. http://dx.doi.org/10.1016/j.molbiopara.2008.12.005
Katulaemail, K.S., Heinloth, A.N. and Paules, R.S. (2007) Folate Deficiency in Normal Human Fibroblasts Leads to Altered Expression of Genes Primarily Linked to Cell Signaling, the Cytoskeleton and Extracellular Matrix. Journal of Nutritional Biochemistry, 18, 541-552. http://dx.doi.org/10.1016/j.jnutbio.2006.11.002
Perveen, R., Ali, Q., Ashraf, M., Al-Qurainy, F., Jamil, Y. and Raza, A.M. (2010) Effects of Different Doses of Low Power Continuous Wave He-Ne Laser Radiation on Some Seed Thermodynamic and Germination Parameters, and Potential Enzymes Involved in Seed Germination of Sunflower (Helianthus annuus L.). Photochemistry and Photobiology, 86, 1050-1055. http://dx.doi.org/10.1111/j.1751-1097.2010.00782.x
Zhang, J. and Han, R. (2009) Effects of He-Ne Laser and UV-B Radiation on Na+/K+-ATPase Activity in Organelles of Wheat Seedlings. Chinese Bulletin of Botany, 44, 451-456. (In Chinese)
Lydon, J., Teramura, A.H. and Summers, E.G. (1986) Effects of Ultraviolet-B Radiation on Growth and Productivity of Field-Grown Soybean. In: Stratospheric Ozone Reduction, Solar Ultraviolet Radiation and Plant Life, Springer-Verlag, Berlin, 313-325.
Wang, X.H., Hao, J.H. and Han, R. (2008) The Alleviating Effects of He-Ne Laser on Membrane Lipid Peroxidation in Wheat Seedling Exposed to Enhanced Ultraviolet-B Irradiation. Acta Laser Biology Sinica, 17, 317-322. (In Chinese)
Feng, H.Y., An, L.Z., Tan, L.L., Hou, Z.D. and Wang, X.L. (2000) Effects of Enhanced Ultraviolet-B Radiation on Pollen Germination and Tube Growth of Taxa in Vitro. Environmental and Experimental Botany, 43, 45-53. http://dx.doi.org/10.1016/S0098-8472(99)00042-8
Chen, X.B., Wang, D.M., Liu, J. and Wang, Z.X. (2002) Effect of Wheat Leaf Eluant on Microfilaments Cytoskeleton of Mesophyll Cells by Cultured Leaf Rust. Journal of Plant Physiology and Molecular Biology, 28, 344-350. (In Chinese)
Huang, S.J. (2005) Analysis of Plant Microtubule Array Organization, Transition and Plant Microtubule Dynamics in Vitro. Ph.D. Thesis, Chinese Agriculture University, Beijing. (In Chinese)
Yu, R., Yuan, M. and Wang, X.C. (1998) Dynamics and Regulation of Microtubule System. Chinese Bulletin of Botany, 15, 19-29.
Luo, M.Z., Yang, Z.Q., Li, J.B., Xu, H.Y., Li, S.S., Zhang, W., Qian, A.R. and Shang, P. (2013) Calcium Influx through Stretch-Activated Channels Mediates Microfilament Reorganization in Osteoblasts under Simulated Weightlessness. Advances in Space Research, 51, 2058-2068. http://dx.doi.org/10.1016/j.asr.2013.01.009
Staiger, C.J. (2000) Signaling to the Actin Cytoskeleton in Plants. Annual Review of Plant Physiology and Plant Molecular Biology, 51, 257-288. http://dx.doi.org/10.1146/annurev.arplant.51.1.257
Wang, L., Ma, C.H. and Zhao, J.X. (2003) The Effects of HMC-Toxin on the Protoplasts and Their Actin Filaments Distribution in Detached Root Cap Cells of Charrua Cytoplasmic Male Sterility Maize. Acta Phytopathologica Sinica, 33, 225-228. (In Chinese)
Lei, Y.H., Ma, C.H. and Wei, J.K. (2004) Effect of Pyricularia Oryzae Toxins on Distribution of Microfilament in Rice Root Cap Cells. Journal of Biology, 21, 17-18. (In Chinese)
Ren, H.Y., Huang, Z.L., Chen, Z.L., Yuan, M. and Lu, T. (2000) Effect of N+ Ions Implantation on Pollen Germination and Microfilaments. Chinese Science Bulletin, 45, 390-394. (In Chinese)
Yang, L.Y., Han, R. and Sun, Y. (2012) Damage Repair Effect of He-Ne Laser on Wheat Exposed to Enhanced Ultraviolet-B Radiation. Plant Physiology and Biochemistry, 57, 218-221.
Zhang, Q. and Han, R. (2006) The Damage Repair Effects of He-Ne Laser on Intact Chloroplasts (in Vitro) Exposed to Enhanced UV-B Radiation. Cell Research, 16, 11.
Han, R., Wang, X.L., Yue, M. and Qi, Z. (2002) Effects of the Enhanced UV-B Radiation on the Body Cell Mitosis of the Wheat. Acta Genetica Sinica, 29, 537-541. (In Chinese)
Wyatt, S.E. and Carpita, N.C. (1993) The Plant Cytoskeleton-Cell-Wall Continuum. Trends in Cell Biology, 3, 413-417. http://dx.doi.org/10.1016/0962-8924(93)90022-S