Derivation Method for Determining Sorbitol in Fruit Trees
- 1 University of São Paulo State (UNESP), Ilha Solteira Campus, Ilha Solteira, Brazil
- 2 University of São Paulo State (UNESP), Ilha Solteira Campus, Ilha Solteira, Brazil
- 3 University of São Paulo State (UNESP), Ilha Solteira Campus, Ilha Solteira, Brazil
- 4 University of São Paulo State (UNESP), Ilha Solteira Campus, Ilha Solteira, Brazil
Abstract
The goal of this study is to describe a methodology for the analysis of D-sorbitol using benzyl alcohol as derivatizing agent and liquid chromatography equipped with a diode-array detector (HPLC-DAD). The study was performed at the University of S ã o Paulo State (UNESP), Ilha Solteira Campus, Brazil. The axillary buds of fruit trees were collected from the UNESP Teaching and Research Farm. Benzyl alcohol was used as derivatizing agent to extract D-sorbitol from the buds. Next, the D-sorbitol underwent solid phase extraction (SPE) using C18 cartridges and was then analyzed by liquid chromatography (HPLC-DAD). The HPLC-DAD derivatization method proposed showed excellent chromatography resolution and high accuracy in the separation of D-sorbitol (derivatized) for the axillary buds of avocado, black mulberry, Japanese pear and peach and also enabled eliminating the use of hazardous derivatizing agents.
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