<i>In Vitro</i> Direct Organogenesis Protocol for Mass Propagation of an Elite Ethiopian Hot Pepper (<i>Capsicum annuum</i> L.) Cultivar: Mareko Fana
- 1 Department of Biology, Adama Science and Technology University, Adama, Ethiopia
- 2 Department of Biology, Adama Science and Technology University, Adama, Ethiopia
- 3 Department of Biology, Adama Science and Technology University, Adama, Ethiopia
Abstract
An efficient and reproducible in vitro mass propagation protocol was developed for Mareko Fana cultivar of hot pepper ( C. annuum L.) through direct organogenesis using nodal and shoot tip explants. Three percent active chlorine for 20 minutes was found to be optimum treatment combination yielding 82.5% ± 5.00% contaminant-free germinated seedlings. For shoot induction, MS + 4.5 mg/l BAP + 0.5 mg/l IAA and MS medium containing eight mg/l Zeatin were found to be optimum resulting 77.5% ± 5.00% and 67.50% ± 5.00% induction percentage for nodal and shoot tip explants respectively. Maximum shoot multiplication responses were obtained on MS + 3 mg/l BAP + 2 mg/l Kinetin with mean number of 9.2 ± 0.2 and 8.6 ± 0.00 shoots for nodal and shoot tip explants respectively. Best shoot elongation and rooting responses were obtained on MS + 0.5 mg/l IBA resulting mean value of 29.6 ± 0.12 root number, 4.25 ± 0.20 cm root length and 5.12 ± 0.20 cm shoot height. The plantlets showed 77.5% survival during acclimatization and transplanting.
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