Seed Germination and <i>in Vitro</i> Organogenesis Optimization of <i>Zhumeria majdae</i> (Lamiaceae) from Iran

Abstract

Zhumeria majdae is a medicinal plant and endemic of Iran that is being threatened to extinction. The present investigation to preserve this relict species, was conducted to study of: 1) different treatments effect on seeds germination: a) in vivo , b) in vitro were subjected to 5 treatments, 2) callus induction and organogenesis from leaf, root and stem of explants by tissue culture on MS medium supplemented with 7 different concentration and combinations of auxin and cytokinin. Results indicated the germination rate were promoted by seeds mucilage, and were inhibited under cold condition. The best callus induction were observed from stem and leaf explants in the MS + B(IAA(0.1) + Kin(1) mg &middot L ^-10 ). The maximum leaf formation of leaf explants were produced in the MS + E(IAA(0.1) + BA(2) mg &middot L ^-10 ).