The Aromatic Plant <i>Satureja horvatii</i> ssp. <i>macrophylla</i> Induces Apoptosis and Cell Death to the A549 Cancer Cell Line — Oak Academic Publishing
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The Aromatic Plant <i>Satureja horvatii</i> ssp. <i>macrophylla</i> Induces Apoptosis and Cell Death to the A549 Cancer Cell Line
Department of Agricultural Technology, Technological Education Institute of Epirus, Arta, Greece
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Medical School, University of Ioannina, Ioannina, Greece
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Department of Agricultural Technology, Technological Education Institute of Epirus, Arta, Greece
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Chemistry Department, University of Ioannina, Ioannina, Greece
1 Department of Agricultural Technology, Technological Education Institute of Epirus, Arta, Greece
2 Medical School, University of Ioannina, Ioannina, Greece
3 Department of Agricultural Technology, Technological Education Institute of Epirus, Arta, Greece
4 Chemistry Department, University of Ioannina, Ioannina, Greece
S. horvatii ssp. macrophylla (Lamiaceae) is an endemic aromatic plant of Greece and is used as a culinary herb. In the present study we explored its possible anticancer activity through the induction of apoptosis on cancer cells using A549 cell line as a model. The cytotoxic effects and the type of cell death (apoptosis/necrosis) were evaluated with biochemical and morphological criteria. The total methanol extract of S. horvatii ssp. macrophylla revealed forty two components, of which carvacrol was the major constituent (43.2%). Only a minor portion of them (3.41%) was contained in the active working plant extract solution which was used for cell treatment. This study indicates that extract of S. horvatii spp. macrophylla , which belongs to carvacrol chemotype, can induce both apoptosis and necrosis to A549 cancer cell line.
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