Research ArticleOpen AccessGoogle Scholar indexed
Phylogenetic Study of <i>Acacia</i> Species Using the Molecular Marker
Department of Biology, College of Science and Arts, Al-Baha University, Baljurashi, Saudi Arabia
- 1 Department of Biology, College of Science and Arts, Al-Baha University, Baljurashi, Saudi Arabia
American Journal of Plant Sciences·Volume 06 (2015)·Pages 3139–3143·Published 2 December 2015·DOI10.4236/ajps.2015.619305
Copy link · social · email
Abstract
Acacia species are found at various arid and semiarid regions. Among the tree genera of family, Fabaceae, Acacia contains the highest number of species. We have collected different species of Acacia from various places of Saudi Arabia and reconstructed phylogeny for evaluation of genetic relationship among them. Internal transcribed spacer sequence of nrDNA (nrDNA-ITS) locus was used for the reconstruction of phylogeny among these species. Based on phylogenetic tree, Acacia etbaica and A. johnwoodii were close to each other. Similarly, A. ehrenbergiana and A. tortilis were close to each other. Thus, this gene locus was helpful in evaluating the genetic relationship among these species.
KeywordsnrDNA-ITSMolecular MarkerGenomic DNAPCR
- Ibrahim, A.A.M. and Aref, I.M. (2000) Host Status of Thirteen Acacia Species to Meloidogyne jovanica. Journal of Nematology, 32, 609-613.
- Aref, I.M. (2000) Morphological Characteristics of Seeds and Seedling Growth of Some Native Acacia Trees in Saudi Arabia. Journal of King Saud University, Agricultural Sciences, 12, 31-95.
- Sahni, M. (1968) Important Trees of the Northern Sudan. Khartoum University Press, 40-63.
- Elkhalifa, K.F. (1996) Forest Botany. Khartoum University Press, 79-94.
- Williames, J.K., Kubelik, A., Livak, K., Rafalsky, J. and Tingey, S. (1990) DNA Polymorphisms Amplified by Arbitrary Primer and Useful as Genetic Markers. Nucleic Acids Research, 18, 6531-6535. http://dx.doi.org/10.1093/nar/18.22.6531
- Vos, R., Hogers, R., Bleeker, M., Reijans, M., Lee, T., Hornes, M., Frijters, A., Pot, J., Peleman, J. and Kuiper, M. (1995) AFLP: A New Technique for DNA Fingerprinting. Nucleic Acids Research, 23, 4407-4414. http://dx.doi.org/10.1093/nar/23.21.4407
- Beckman, J.S. and Soller, M. (1983) Restriction Fragment Length Polymorphisms in Genetic Improvement: Methodologies, Mapping and Costs. Theoretical and Applied Genetics, 67, 35-43. http://dx.doi.org/10.1007/BF00303919
- Butcher, P.A., Moran, G.F. and Perkins, H.D. (1998) RFLP Diversity in the Nuclear Genome of Acacia mangium. Heredit, 81, 205-213. http://dx.doi.org/10.1046/j.1365-2540.1998.00392.x
- McGranahan, M., Bell, J.C., Moran, G.F. and Slee, M. (1997) High Genetic Divergence between Geographic Regions in the Highly out Crossing Species Acacia aulacocarpa (Cunn. Ex Benth.). Forest Genetics, 4, 1-13.
- Playford, J., Bell, J.C. and Moran, G.F. (1993) A Major Disjunction in Genetic Diversity over the Geographic Range of Acacia Melanoxylon R. Australian Journal of Botany, 41, 355-368. http://dx.doi.org/10.1071/BT9930355
- Ashworth, S. (2002) Seed to Seed. 2nd Edition. Seed Savers Exchange, Decorah.
- Tamura, K., Peterson, D., Peterson, N., Stecher, G., Nei, M. and Kumar, S. (2011) MEGA5: Molecular Evolutionary Genetics Analysis Using Maximum Likelihood, Evolutionary Distance, and Maximum Parsimony Methods. Molecular Biology and Evolution, 28, 2731-2739. http://dx.doi.org/10.1093/molbev/msr121
- Thompson, J.D., Gibson, T.J., Plewniak, F., Jeanmougin, F. and Higgins, D.G. (1997) The Clustal X Windows Interface: Flexible Strategies for Multiple Sequence Alignment Aided by Quality Analysis Tools. Nucleic Acids Research, 24, 4876-4882. http://dx.doi.org/10.1093/nar/25.24.4876