Determination of the Genetic Structure of the Oleaginous <i>Lagenaria siceraria</i> of the Nangui Abrogoua University Germplasm Collection — Oak Academic Publishing
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Determination of the Genetic Structure of the Oleaginous <i>Lagenaria siceraria</i> of the Nangui Abrogoua University Germplasm Collection
Laboratoire de Génomique Fonctionnelle et Amélioration Génétique (LaGeFAGe), UFR-SN, Université Nangui Abrogoua, Abidjan, Côte d’Ivoire
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Laboratoire de Génomique Fonctionnelle et Amélioration Génétique (LaGeFAGe), UFR-SN, Université Nangui Abrogoua, Abidjan, Côte d’Ivoire
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Unité de Phytotechnie Tropicale et d’Horticulture, Gembloux Agro Bio Tech, Université de Liège, Gembloux, Belgium
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Laboratoire de Génomique Fonctionnelle et Amélioration Génétique (LaGeFAGe), UFR-SN, Université Nangui Abrogoua, Abidjan, Côte d’Ivoire
1 Laboratoire de Génomique Fonctionnelle et Amélioration Génétique (LaGeFAGe), UFR-SN, Université Nangui Abrogoua, Abidjan, Côte d’Ivoire
2 Laboratoire de Génomique Fonctionnelle et Amélioration Génétique (LaGeFAGe), UFR-SN, Université Nangui Abrogoua, Abidjan, Côte d’Ivoire
3 Unité de Phytotechnie Tropicale et d’Horticulture, Gembloux Agro Bio Tech, Université de Liège, Gembloux, Belgium
4 Laboratoire de Génomique Fonctionnelle et Amélioration Génétique (LaGeFAGe), UFR-SN, Université Nangui Abrogoua, Abidjan, Côte d’Ivoire
Thirty accessions of Lagenaria siceraria from the Nangui Abrogoua University germplasm collection were analyzed using three microsatellite markers. The average Polymorphism Information Content (PIC) value was 0.61. The average observed heterozygosity value (H0 = 0.631) compared to the average expected heterozygosity value (He = 0.645) did not show significant differences in the selected accessions, which suggested random mating in the set of accessions. Within accession inbreeding estimate (FIS), was 40% and was not significantly different from zero. The reduction of heterozygotes was likely the result of presence of null alleles. Analysis of Molecular Variance (AMOVA) within and among 30 accessions of L. siceraria revealed that 39% of the total variation resides among accessions and 61% within accessions. Accession structuring pattern derived from Bayesian clustering analysis revealed two clusters. Based on the genetic structure of the accessions analyzed, a sampling strategy to collect and conserve genetic resources of L. siceraria was suggested.
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