The genetic diversity among 15 NIFOR breeding parents was assessed using 10 microsatellite markers. A high genetic diversity was observed with a total of 64 alleles including 23 rare alleles or alleles at frequencies less than 0.05. The NIFOR tenera parents recorded the highest number of rare alleles. The average observed heterozygosity and mean gene diversity across all parental groups were 0.6889 and 0.7029, respectively. Higher genetic diversity was detected among the NIFOR dura and tenera parents compared to that of the Deli dura parents in absolute terms. Analysis of molecular variance (AMOVA) showed that 87% of the total variation ( p < 0.001) observed was due to differences among parents. Rogers’ genetic distance ranged from 0.2988 to 0.8000 (mean = 0.5570). The dendrogram constructed on the basis of Rogers’ genetic distance clustered the parents in three groups. They generally clustered in heterotic manner rather than by geographic origins. The groupings obtained through PCoA confirmed the results obtained by cluster analysis. The results obtained are strong assets for NIFOR breeding programme.
[1] Comstock, R.E., Robinson, H.F. and Harvey, P.H. (1949) A Breeding Procedure Designed to Make Maximum Use of Both General and Specific Combining Ability. Journal of the American Society of Agronomy, 41, 360-367. http://dx.doi.org/10.2134/agronj1949.00021962004100080006x
Okwuagwu, C.O. (1986) The Genetic Basis of the NIFOR Oil Palm Breeding Programme. Proceedings of the International Workshop on Oil Palm Germplasm and Utilization, Selangor, 26-27 March 1986, 228-237.
West, M.J. (1976) The Analysis of the Bunch Yield Data of the NIFOR Oil Palm Breeding Programme and the Choice of New Parental Material. A Supplementary Report of the Ministry of Overseas Development on Research Project, R2354, Mimeo, 41.
West, M.J., Ross, J.M., Obasola, C.O. and Mekako, H.U. (1976) The Inheritance of Yield and of Fruit and Bunch Composition Characters in the Oil Palm—An Analysis of the NIFOR Main Breeding Programme. Proceedings of the Malaysian International Agricultural Oil Palm Conference, Kuala Lumpur, 14-17 June 1976, 1-11.
Okwuagwu, C.O. (1989) The Developments from the Comprehensive Oil Palm Breeding Programme of the Nigerian Institute for Oil Palm Research (NIFOR) and Future Breeding Strategy. Proceedings of the NIFOR International Conference on Palms and Palm Products, 1, 10-24.
Okwuagwu, C.O. and Ataga, C.D. (1992) Oil Palm Breeding Programme in Nigeria. Proceedings of the ISOPB International Symposium on the Science of Oil Palm Breeding, Montpellier, 1-3 July 1992, 131-140.
Okwuagwu, C.O. and Okoye, M.N. (2006) Developments from the NIFOR Second Cycle Oil Palm Main Breeding and Selection Programme—The Plan of Third Cycle Programme. NIFOR Seminar Presentation, June 2006.
Cochard, B., Adon, B., Rekima, S., Billotte, N., Desmier, R., Koutou, A., et al. (2009) Geographic and Genetic Structure of African Oil Palm Diversity Suggests New Approaches to Breeding. Tree Genetics & Genomes, 5, 493-504. http://dx.doi.org/10.1007/s11295-009-0203-3
NIFOR (1974) The Genepool. Nigerian Institute for Oil Palm Research, Annual Report No. 10, 36-40.
Obasola, C.O., Arasu, N.T. and Rajanaidu, N. (1980) Collection of Oil Palm Genetic Material in Nigeria. I. Method of Collection. MARDI Report No. 80.
Okwuagwu, C.O., Ataga, C.D. and Okolo, E.C. (1993) Collecting Oil Palm Germplasm in Nigeria. FAO/IBPGR Plant Genetic Resources Newsletter, 93, 38-39.
Ataga, C.D., Okwuagwu, C.O., Okolo, E.C. and Okoye, M. (2003) Interrelationships among Vegetative, Bunch and Fruit Traits in an Oil Palm Germplasm Collection. In: Rajanaidu, N., et al., Eds., Proceedings of the 2003 PIPOC International Palm Oil Congress, Malaysian Palm Oil Board, Kuala Lumpur, A-PA.
Okwuagwu, C.O. and Okoye, M.N. (2003) The Second Cycle of the NIFOR Oil Palm Main Breeding Programme. Technical Report, Nigerian Institute for Oil Palm Research (NIFOR), Benin City.
Okoye, M.N. (2008) Stability and Genetic Variability of Bunch Yield Components of NIFOR Second Cycle Oil Palm Hybrids. Master’s Thesis, University of Nigeria, Nsukka.
Okwuagwu, C.O., Okoye, M.N., Okolo, E.C., Ataga, C.D. and Uguru, M.I. (2008) Genetic Variability of Fresh Fruit Bunch Yield in Deli/dura x tenera Breeding Populations of Oil Palm (Elaeis guineensis Jacq.) in Nigeria. Journal of Tropical Agriculture, 46, 40-45.
Okwuagwu, C.O., Ataga, C.D., Okoye, M.N. and Okolo, E.C. (2011) Germplasm Collection of Highland Palms of Afikpo in Eastern Nigeria. Bayero Journal of Pure and Applied Sciences, 4, 112-114. http://dx.doi.org/10.4314/bajopas.v4i1.25
Smith, J.S.C. and Smith, O.S. (1992) Fingerprinting Crop Varieties. Advanced Agronomy, 47, 85-140. http://dx.doi.org/10.1016/S0065-2113(08)60489-7
Pinto, L.R., Vieira, M.L.C. and Souza Jr., C.L. (2003) Reciprocal Recurrent Selection Effects on the Genetic Structure of Tropical Maize Populations Assessed at Microsatellite Loci. Genetics and Molecular Biology, 26, 355-364. http://dx.doi.org/10.1590/S1415-47572003000300022
Berilli, A.P.C.G., Pereira, M.G., Gonçalves, L.S.A., da Cunha, K.S., Ramos, H.C.C., Souza Filho, G.A. and do Amaral Júnior, A.T. (2011) Use of Molecular Markers in Reciprocal Recurrent Selection of Maize Increases Heterosis Effects. Genetics and Molecular Research, 10, 2589-2596. http://dx.doi.org/10.4238/2011.October.25.6
Mohan, M., Nair, S., Bhagwat, A., Krishna, T.G., Yano, M., Bhatia, C.R. and Sasaki, T. (1997) Genome Mapping, Molecular Markers and Marker-Assisted Selection in Crop Plants. Molecular Breeding, 3, 87-103. http://dx.doi.org/10.1023/A:1009651919792
Mayes, S., Hafeez, F., Price, Z., MacDonald, D., Billotte, N. and Roberts, J. (2008) Molecular Research in Oil Palm, the Key Oil Crop for the Future. In: Moore, P.H. and Ming, R., Eds., Genomics of Tropical Crop Plants, Springer, New York, 371-404. http://dx.doi.org/10.1007/978-0-387-71219-2_15
Mondini, L., Noorani, A. and Pagnotta, M.A. (2009) Assessing Plant Genetic Diversity by Molecular Tools. Diversity, 1, 19-35. http://dx.doi.org/10.3390/d1010019
Purba, A.R., Noyer, J.L., Baudouin, L., Perrier, X., Hamon, S. and Lagoda, P.J.L. (2000) A New Aspect of Genetic Diversity If Indonesian Oil Palm (Elaeis guineensis Jacq.) Revealed by Isoenzyme and AFLP Markers and Its Consequences for Breeding. Theoretical and Applied Genetics, 101, 956-961. http://dx.doi.org/10.1007/s001220051567
Ghesquiére, M. (1985) Enzyme Polymorphism in Oil Palm (Elaies guineensis Jacq.) II Variability and Genetic Structure of Seven Origins of Oil Palm. Oléagineux, 40, 529-540.
Shah, F.H., Rashid, O., Simons, A.J. and Dunsdon, A. (1994) The Utility of RAPD Markers for the Determination of Genetic Variation in the Oil Palm (Elaeis guineensis). Theoretical and Applied Genetics, 89, 713-718. http://dx.doi.org/10.1007/BF00223710
Rafalski, J.A. (1997) Randomly Amplified Polymorphic DNA (RAPD) Analysis. In: Caetano-Anolles, G. and Gresshoff, P.M., Eds., DNA Markers Protocols, Applications and Overviews, Wiley-Vch, New York, 75-83.
Maizura, I., Rajanaidu, N., Zakri, A. and Cheah, S. (2006) Assessment of Genetic Diversity in Oil Palm (Elaeis guineensis Jacq) Using Restriction Fragment Length Polymorphism (RFLP). Genetic Resources and Crop Evolution, 53, 187-195.
Kularatne, R.S. (2000) Assessment of Genetic Diversity in Natural Oil Palm (Elaeis guineensis Jacq.) Using Amplified Fragment Length Polymorphism Markers. PhD Thesis, Universiti Kebangsaan Malaysia, Bangi.
Gupta, P.K., Balyan, H.S., Sharma, P.C. and Ramesh, B. (1996) Microsatellites in Plants: A New Class of Molecular Markers. Current Science, 70, 45-54.
Akkaya, M.S., Bhagwat, A.A. and Cregan, P.B. (1992) Length Polymorphisms of Simple Sequence Repeat DNA in Soybean. Genetics, 132, 1131-1139.
Gupta, P.K. and Varshney, R.K. (2000) The Development and Use of Microsatellite Markers for Genetic Analysis and Plant Breeding with the Emphasis on Bread Wheat. Euphytica, 113, 163-185. http://dx.doi.org/10.1023/A:1003910819967
Zane, L., Bargelloni, L. and Patarnello, T. (2002) Strategies for Microsatellite Isolation: A Review. Molecular Ecology, 11, 1-16. http://dx.doi.org/10.1046/j.0962-1083.2001.01418.x
Morgante, M. and Olivieri, A.M. (1993) PCR-Amplified Microsatellites as Markers in Plant Genetics. The Plant Journal, 3, 175-182. http://dx.doi.org/10.1111/j.1365-313X.1993.tb00020.x
Peakall, R., Gilmore, S., Keys, W., Morgante, M. and Rafalski, A. (1998) Cross-Species Amplification of Soybean (Glycine max) Simple Sequence Repeats (SSRs) within the Genus and Other Legume Genera: Implications for the Transfer Ability of SSRs in Plants. Molecular Biology and Evolution, 15, 1275-1287. http://dx.doi.org/10.1093/oxfordjournals.molbev.a025856
Saghai-Maroof, M.A., Biyashev, R.M., Yang, G.P., Zhang, Q. and Allard, R.W. (1994) Extraordinarily Polymorphic Microsatellite DNA in Barley: Species Diversity, Chromosomal Locations, and Population Dynamics. Proceedings of the National Academy of Sciences of the United States of America, 91, 5466-5470. http://dx.doi.org/10.1073/pnas.91.12.5466
Billotte, N., Rusterucci, A., Barcelos, E., Noyer, J., Amblard, P. and Baurens, F. (2001) Development, Characterization, and Across-Taxa Utility of Oil Palm (Elaeis guineensis Jacq.) Microsatellite Markers. Genome, 44, 413-425. http://dx.doi.org/10.1139/gen-44-3-413
Bakoumé, C., Wickneswari, R., Rajanaidu, N., Kushairi, A., Amblard, P. and Billotte, N. (2007) Allelic Diversity of Natural Oil Palm (Elaeis guineensis Jacq.) Populations Detected by Microsatellite Markers: Implication for Conservation. Plant Genetic Resources: Characterization and Utilization, 5, 104-107. http://dx.doi.org/10.1017/S1479262107710870
Bakoumé, C., Wickneswari, R., Siju, S., Rajanaidu, N., Kushairi, A. and Billotte, N. (2015) Genetic Diversity of the World’s Largest Oil Palm (Elaeis guineensis Jacq.) Field Genebank Accessions Using Microsatellite Markers. Genetic Resources and Crop Evolution, 62, 349-360. http://dx.doi.org/10.1007/s10722-014-0156-8
Singh, R., Noorhariza, M.Z., Ting, N.C., Rozana, R., Tan, S.G., Low, L.E.T., et al. (2008) Exploiting an Oil Palm EST the Development of Gene-Derived and Their Exploitation for Assessment of Genetic Diversity. Biologia, 63, 1-9. http://dx.doi.org/10.2478/s11756-008-0041-z
Arias, D., Montoya, C., Rey, L. and Romero, H. (2012) Genetic Similarity among Commercial Oil Palm Materials Based on Microsatellite Markers. Agronomia Colombiana, 30, 188-195.
Taeprayoon, P., Tanya, P., Suk-Ha, L. and Srinives, P. (2015) Genetic Background of Three Commercial Oil Palm Breeding Populations in Thailand Revealed by SSR Markers. Australian Journal of Crop Science, 9, 281-288.
Singh, R., Nagappan, J., Tan, S.-G., Panandam, J.M. and Cheah, S.-C. (2007) Development of Simple Sequence Repeat (SSR) Markers for Oil Palm and Their Application in Genetic Mapping and Fingerprinting of Tissue Culture Clones. Asia-Pacific Journal of Molecular Biology and Biotechnology, 15, 121-131.
Norziha, A., Rafii, M., Maizura, I. and Ghizanm, S. (2008) Genetic Variation among Oil Palm Parent Genotypes and Their Progenies Based on Microsatellite Markers. The Journal of Oil Palm Research, 20, 533-541.
Thawaro, S. and Te-Chato, S. (2010) Verification of Legitimate Tenera Oil Palm Hybrids Using SSR and Propagation of Hybrids by Somatic Embryogenesis. Songklanakarin Journal of Science and Technology, 32, 1-8.
Bakoumé, C., Aziah, M.Y., Praveena, T., Teh, C.K., Suzaini, Y., Hamidah, M., et al. (2011) DNA Sequence-Based Markers for Verification of Ramet-to-Ortet Relationship in Oil Palm (Elaeis guineensis Jacq.). American Journal of Plant Sciences, 2, 539-548. http://dx.doi.org/10.4236/ajps.2011.24064
Hama-Ali, E.O., Alwee, S.S.R.S., Tan, G.S., Panandam, J.M., Ling, H.C., Namasivayam, P. and Peng, H.B. (2015) Illegitimacy and Sibship Assignments in Oil Palm (Elaeis guineensis Jacq.) Half-Sib Families Using Single Locus DNA Microsatellite Markers. Molecular Biology Reports, 42, 917-925. http://dx.doi.org/10.1007/s11033-014-3829-7
Billotte, N., Jourjon, M., Marseillac, N., Berger, A., Flori, A., Asmady, H., et al. (2010) QTL Detection by Multi-Parent Linkage Mapping in Oil Palm (Elaeis guineensis Jacq.). Theoretical and Applied Genetics, 120, 1673-1687. http://dx.doi.org/10.1007/s00122-010-1284-y
Montoya, C., Lopes, R., Flori, A., Cros, D., Cuellar, T., Summo, M., et al. (2013) Quantitative Trait Loci (QTLs) Analysis of Palm Oil Fatty Acid Composition in an Interspecific Pseudo-Backcross from Elaeis oleifera (HBK) Cortés and Oil Palm (Elaeis guineensis Jacq.). Tree Genetics & Genomes, 9, 1207-1225. http://dx.doi.org/10.1007/s11295-013-0629-5
Ting, N.C., Jansen, J., Nagappan, J., Ishak, Z., Chin, C.-W., Tan, S.G., et al. (2013) Identification of QTLs Associated with Callogenesis and Embryogenesis in Oil Palm Using Genetic Linkage Maps Improved with SSR Markers. PLoS ONE, 8, e53076. http://dx.doi.org/10.1371/journal.pone.0053076
Doyle, J.J. and Doyle, J.L. (1990) Isolation of Plant DNA from Fresh Tissue. Focus, 12, 13-15.
Ting, N.C., Noorhariza, M.Z., Rozana, R., Low, E.T., Ithnin, M., Cheah, S.C., et al. (2010) SSR Mining in Oil Palm EST Database: Application in Oil Palm Germplasm Diversity Studies. Journal of Genetics, 89, 135-145. http://dx.doi.org/10.1007/s12041-010-0053-7
Billotte, N., Marseillac, N., Risterucci, A., Adon, B., Brottier, P., Baurens, F., et al. (2005) Microsatellite-Based High Density Linkage Map in Oil Palm (Elaeis guineensis Jacq.). Theoretical and Applied Genetics, 110, 754-765. http://dx.doi.org/10.1007/s00122-004-1901-8
Crow, J.F. and Kimura, M. (1970) An Introduction to Population Genetic Theory. Harper and Row, New York.
Marshall, D.R. and Brown, A.H.D. (1975) Optimum Sampling Strategy in Genetic Conservation. In: Frankel, O.H. and Hawkes, J.G., Eds., Crop Genetic Resources for Today and Tomorrow, Cambridge University Press, London, 53-80.
Botstein, D., White, R.L., Skolnick, M. and Davis, R.W. (1980) Construction of a Genetic Linkage Map in Man Using Restriction Fragment Length Polymorphisms. The American Journal of Human Genetics, 32, 314-331.
Nei, M. (1972) Genetic Distance between Populations. American Naturalist, 106, 283-292. http://dx.doi.org/10.1086/282771
Liu, K. and Muse, S.V. (2005) PowerMarker: An Integrated Analysis Environment for Genetic Marker Analysis. Bioinformatics, 21, 2128-2129. http://dx.doi.org/10.1093/bioinformatics/bti282
Rogers, J.S. (1972) Measures of Genetic Similarity and Genetic Distance. In: Studies in Genetics VII, University of Texas Publication 7213, Austin, 145-153.
Sneath, P.H.A. and Sokal, R.R. (1973) Numerical Taxonomy: The Principles and Practice of Numerical Classification. W. H. Freeman, San Francisco.
Tamura, K., Dudley, J., Nei, M. and Kumar, S. (2007) MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) Software Version 4.0. Molecular Biology and Evolution, 24, 1596-1599. http://dx.doi.org/10.1093/molbev/msm092
Perrier, X. and Jacquemoud-Collet, J.P. (2006) DARwin Software. http://darwin.cirad.fr/
Peakall, R. and Smouse, P.E. (2006) GenAlEx 6: Genetic Analysis in Excel. Population Genetic Software for Teaching and Research. Molecular Ecology Notes, 6, 288-295. http://dx.doi.org/10.1111/j.1471-8286.2005.01155.x
Peakall, R. and Smouse, P.E. (2012) GenAlEx 6.5: Genetic Analysis in Excel. Population Genetic Software for Teaching and Research—An Update. Bioinformatics Application Note, 28, 2537-2539. http://dx.doi.org/10.1093/bioinformatics/bts460
Wright, S. (1965) The Interpretation of Population Structure by F-Statistics with Special Regard to System of Mating. Evolution, 19, 395-420. http://dx.doi.org/10.2307/2406450
Excoffier, L., Smouse, P.E. and Quattro, J.M. (1992) Analysis of Molecular Variance Inferred from Metric Distances among DNA Haplotypes: Application to Human Mitochondrial DNA Restriction Data. Genetics, 131, 479-491.
Weber, J.L. and Wong, C. (1993) Mutation of Human Short Tandem Repeats. Human Molecular Genetics, 2, 1123-1128. http://dx.doi.org/10.1093/hmg/2.8.1123
Zeng, L., Kwon, T.R., Liu, X., et al. (2004) Genetic Diversity Analyzed by Microsatellite Markers among Rice (Oryza sativa L.) Genotypes with Different Adaptations to Saline Soils. Plant Science, 116, 1275-1285. http://dx.doi.org/10.1016/j.plantsci.2004.01.005
Hayati, A., Wickneswari, R., Maizura, I. and Rajanaidu, N. (2004) Genetic Diversity of Oil Palm (Elaeis guineensis Jacq.) Germplasm Collections from Africa: Implications for Improvement and Conservation of Genetic Resources. Theoretical and Applied Genetics, 108, 1274-1284. http://dx.doi.org/10.1007/s00122-003-1545-0
Varshney, R.K., Sorrells, M.E. and Graner, A. (2005) Genic Microsatellite Markers in Plants: Features and Applications. Trends in Biotechnology, 23, 48-55. http://dx.doi.org/10.1016/j.tibtech.2004.11.005
Thongthawee, S., Tittinutchanon, P. and Volkaert, H. (2010) Microsatellites for Parentage Analysis in an Oil Palm Breeding Population. Thai Journal of Genetics, 3, 172-181.
Allou, D., Adon, B. and Sangare, A. (2008) Molecular Variability from Two Selection of BRT10 Population in an Inbreeding Program of Oil Palm (Elaeis guineensis Jacq.) in Côte d’Ivoire. African Journal of Biotechnology, 7, 3550-3553.
Agustina, L., Rivallan, R., Zulhermanna, Puspitaningrum, Y., Sudarsono, Perrier, X., et al. (2010) Allelic Diversity of 22 Sampoerna Agro’s Oil Palm Pisifera Based on Microsatellite Markers. Proceedings of the International Oil Palm Conference, Yogyakarta, 1-3 June 2010, 9.
Rajanaidu, N., Kushairi, A., Rafii, M., Din M., Maizura, I. and Jalani, B.S. (2000) Oil Palm Breeding and Genetic Resources. In: Basiron, Y., Jalani, B.S. and Chan, K.W., Eds., Advances in Oil Palm Research, Malaysian Palm Oil Board, Kuala Lumpur, 171-227.
Nybom, H. (2004) Comparison of Different Nuclear DNA Markers for Estimating Intraspecific Genetic Diversity in Plants. Molecular Ecology, 13, 1143-1155. http://dx.doi.org/10.1111/j.1365-294X.2004.02141.x
Noorhariza, M.Z., Ismanizan, I., Rozana, R., Ting, N.C. and Singh, R. (2010) Development and Characterization of Elaeis oleifera Microsatellite Markers. Sains Malaysiana, 39, 909-912.
Zulkifli, Y., Maizura, I. and Rajinder, S. (2012) Evaluation of MPOB Oil Palm Germplasm (Elaeis guineensis) Populations Using EST-SSR. Journal of Oil Palm Research, 24, 1368-1377.
Bruford, M.W., Hanotte, O., Brookfield, J.F.Y. and Burke, T. (1998) Multi-Locus and Single-Locus DNA Fingerprinting. In: Hölzel, A.R., Ed., Molecular Genetic Analysis of Populations: A Practical Approach, IRL Press, Oxford, 287-336.
Hamrick, J.L., Godt, M.J.W. and Sherman-Broyles, S.L. (1992) Factors Influencing Levels of Genetic Diversity in Woody Plant Species. New Forests, 6, 95-124. http://dx.doi.org/10.1007/BF00120641
Wright, S. (1978) Evolution and the Genetics of Population, Variability within and among Natural Populations. The University of Chicago Press, Chicago.
Hartl, D.L. and Clark, A.G. (1997) Principles of Population Genetics. 3rd Edition, Sinauer Associates, Inc. Publishers, Sunderland.
Reif, J.C., Melchinger, A.E. and Frisch, M. (2005) Genetical and Mathematical Properties of Similarity and Dissimilarity Coefficients Applied in Plant Breeding and Seed Bank Management. Crop Science, 45, 1-7. http://dx.doi.org/10.2135/cropsci2005.0001
Li, Y.C., Korol, A.B., Fahima, T., Beiles, A. and Nevo, E. (2002) Microsatellites: Genomic Distribution, Putative Functions, and Mutational Mechanisms (A Review). Molecular Ecology, 11, 2543-2565. http://dx.doi.org/10.1046/j.1365-294X.2002.01643.x
Barata, C. and Carena, M.J. (2006) Classification of North Dakota Maize Inbred Lines into Heterotic Groups Based on Molecular and Testcross Data. Euphytica, 151, 339-349. http://dx.doi.org/10.1007/s10681-006-9155-y
Senior, M.L., Murphy, J.P., Goodman, M.M. and Stuber, C.W. (1998) Utility of SSRs for Determining Genetic Similarities and Relationships in Maize Using an Agarose Gel System. Crop Science, 38, 1088-1098. http://dx.doi.org/10.2135/cropsci1998.0011183X003800040034x
Mumm, R.H. and Dudley, J.W. (1994) A Classification of 148 US Maize Inbreds: I. Cluster Analysis Based on RFLPs. Crop Science, 34, 842-851. http://dx.doi.org/10.2135/cropsci1994.0011183X003400040005x