Research ArticleOpen AccessGoogle Scholar indexed
ISSR Fingerprinting to Ascertain the Genetic Relationship of <i>Curcuma sp.</i> of Tripura
Department of Botany, Tripura University, Suryamaninagar, India
Department of Botany, Tripura University, Suryamaninagar, India
Department of Molecular Biology and Bioinformatics, Tripura University, Suryamaninagar, India
Department of Botany, Tripura University, Suryamaninagar, India
- 1 Department of Botany, Tripura University, Suryamaninagar, India
- 2 Department of Botany, Tripura University, Suryamaninagar, India
- 3 Department of Molecular Biology and Bioinformatics, Tripura University, Suryamaninagar, India
- 4 Department of Botany, Tripura University, Suryamaninagar, India
American Journal of Plant Sciences·Volume 07 (2016)·Pages 259–266·Published 3 February 2016·DOI10.4236/ajps.2016.72025
Copy link · social · email
Abstract
Molecular fingerprints of four different species of Curcuma, viz., C. amada, C. caesia, C. longa and C. zedoaria , found in Tripura were developed using Inter Simple Sequence Repeats. Twenty ISSR primers generated 116 loci amplified in the range of 200 - 5000 bp with an average of 5.8 alleles and 1.6 effective alleles per locus. The percentage of polymorphic band was found to be 86.29 with an average of 5.15 per primer. Based on UPGMA algorithm these four species are placed in two different clusters that validate the classification based on external and internal morphological characters. The polymorphic ISSR markers generated from this study will be useful for understanding the genetic relationship of different species of the genus Curcuma .
Keywords<i>Curcuma</i>Genetic DiversityInter Simple Sequence RepeatsPolymerase Chain ReactionTaxonomyZingiberaceae
- Skornickova, J.L., Otakar, S., Jarolimova, V., Sabu, M., Fer, T., Travnicek, P. and Suda, J. (2007) Chromosome Numbers and Genome Size Variation in Indian Species of Curcuma (Zingiberaceae). Annals of Botany, 100, 505-526. http://dx.doi.org/10.1093/aob/mcm144
- Velayudhan, K.C., Muralidharan, V.K., Amalraj, V.A., Gautam, P.L., Mandal, S. and Kumar, D. (1999) Curcuma Genetic Resources. Scientific Monograph No. 4, National Bureau of Plant Genetic Resources, New Delhi.
- Mridula, K.R. and Jayachandran, B.K. (2001) Quality of Mango Ginger (Curcuma amada Roxb.) as Influenced by Mineral Nutrition. Journal of Tropical Agriculture, 39, 182-183.
- Matsuda, H., Tewtrakul, S., Morikawa, T., Nakamura, A. and Yoshikawa, M. (2004) Anti-Allergic Principles from Thai Zedoary: Structural Requirements of Curcuminoids for Inhibition of Degranulation and Effect on the Release of TNF-Alpha and IL-4 in RBL-2H3 Cells. Bioorganic & Medicinal Chemistry, 12, 5891-5898. http://dx.doi.org/10.1016/j.bmc.2004.08.027
- Sarma, G.C. and Kalita, S. (2005) Zingiberaceae of Assam: Their Taxonomy and Utilization. Ph.D. Thesis, Botany Department, Gauhati University, Assam.
- Das, A., Kesari, V., Satyanarayana, V.M., Parida, A. and Rangan, L. (2011) Genetic Relationship of Curcuma Species from North East India Using PCR Based Markers. Molecular Biotecnology, 49, 65-76. http://dx.doi.org/10.1007/s12033-011-9379-5
- Larsen, K. and Smith, R.M. (1978). A New Species of Curcuma from Thailand. Notes Royal Botanical Garden. Edinburgh, 30, 269-272.
- Sabu, M. (1991) A Taxonomic and Phylogenetic Study of South Indian Zingiberaceae. Ph.D. Thesis, Department of Botany, University of Calicut.
- Myers, N., Mittermier, R.A., Mittermier, C.G., Fonseca da, G.A.B. and Kent, J. (2000) Biodiversity Hotspots for Conservation Priorities. Nature, 40, 853-858. http://dx.doi.org/10.1038/35002501
- Mao, A.A., Hynniewta, T.M. and Sanjappa, M. (2009) Plant Wealth of Northeast India with Reference to Ethnobotany. Indian Journal of Traditional Knowledge, 8, 96-103.
- Deb, D.B. (1981) The Flora of Tripura. Vol. I & Vol. II, Today and Tomorrow’s Publ., New Delhi.
- Islam, M.A., Meister, A., Schubert, V., Kloppstech, K. and Esch, E. (2006) Genetic Diversity and Cytogenetic Analyses in Curcuma zedoaria (Christm.) Roscoe from Bangladesh. Genetic Resources and Crop Evolution, 54, 149-156. http://dx.doi.org/10.1007/s10722-005-2639-0