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First Report on the Presence of <i>Phyllachora</i> sp. in Corn Crops at Toluca, Estado de Mexico
Universidad Autónoma Agraria Antonio Narro, Saltillo, México
Department of Genetic Engineering, CINVESTAV, Irapuato, México
Department of Agricultural Parasitology, Universidad Autónoma Agraria Antonio Narro, Saltillo, México
Department of Agricultural Parasitology, Universidad Autónoma Agraria Antonio Narro, Saltillo, México
Department of Agricultural Parasitology, Universidad Autónoma Agraria Antonio Narro, Saltillo, México
Department of Agricultural Parasitology, Universidad Autónoma Agraria Antonio Narro, Saltillo, México
Department of Biotechnology and Biochemistry, CINVESTAV, Irapuato, México
General Terán, INIFAP, General Terán, Nuevo León, México
- 1 Universidad Autónoma Agraria Antonio Narro, Saltillo, México
- 2 Department of Genetic Engineering, CINVESTAV, Irapuato, México
- 3 Department of Agricultural Parasitology, Universidad Autónoma Agraria Antonio Narro, Saltillo, México
- 4 Department of Agricultural Parasitology, Universidad Autónoma Agraria Antonio Narro, Saltillo, México
- 5 Department of Agricultural Parasitology, Universidad Autónoma Agraria Antonio Narro, Saltillo, México
- 6 Department of Agricultural Parasitology, Universidad Autónoma Agraria Antonio Narro, Saltillo, México
- 7 Department of Biotechnology and Biochemistry, CINVESTAV, Irapuato, México
- 8 General Terán, INIFAP, General Terán, Nuevo León, México
American Journal of Plant Sciences·Volume 07 (2016)·Pages 733–739·Published 31 March 2016·DOI10.4236/ajps.2016.75067
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Abstract
Symptoms of Tar Spot Complex (TSC) in corn appear in the form of black spots surrounded by a chlorotic halo known as fish-eye. The crop with these symptoms was found at San Pablo Autopan, Estado de Mexico. The stromal region of foliar lesions was cut longitudinally, showing perithecia, asci and ascospores typical of Phyllachora maydis . The results were confirmed by PCR-ITS with ITS 1 and ITS4 initiators, cloned by pJET, before being sequenced and compared against NCBI’s data base. Phyllachora identity was confirmed. This is the first report where TSC pathogen has been detected under conditions that had not been previously reported, for instance, at 2600 meters above the sea level.
Keywords<i>Phyllachora maydis</i>Tar Spot ComplexITS
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