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Differential Expression of miRNAs in <i>Sorghum bicolor</i> under Drought and Salt Stress
Faculty of Agriculture, University of Khartoum, Khartoum North, Sudan
Commission for Biotechnology & Genetic Engineering National Centre for Research, Khartoum, Sudan
Faculty of Agriculture, University of Khartoum, Khartoum North, Sudan
Faculty of Agriculture, University of Khartoum, Khartoum North, Sudan
Faculty of Agriculture, University of Khartoum, Khartoum North, Sudan
Plant Molecular Biology Group, International Centre for Genetic Engineering and Biotechnology, ArunaAsaf Ali Marg, New Delhi, India
- 1 Faculty of Agriculture, University of Khartoum, Khartoum North, Sudan
- 2 Commission for Biotechnology & Genetic Engineering National Centre for Research, Khartoum, Sudan
- 3 Faculty of Agriculture, University of Khartoum, Khartoum North, Sudan
- 4 Faculty of Agriculture, University of Khartoum, Khartoum North, Sudan
- 5 Faculty of Agriculture, University of Khartoum, Khartoum North, Sudan
- 6 Plant Molecular Biology Group, International Centre for Genetic Engineering and Biotechnology, ArunaAsaf Ali Marg, New Delhi, India
American Journal of Plant Sciences·Volume 07 (2016)·Pages 870–878·Published 5 April 2016·DOI10.4236/ajps.2016.76082
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Abstract
The regulatory mechanisms of drought and salt-associated miRNAs have not been fully understood in Sorghum bicolor . In this study, we investigated the effect of salinity stress (200 and 300 mM NaCl) and drought stress at pre- and post-flowering stages on the expression pattern of small regulatory RNAs in six Sorghum genotypes using semi - quantitative reverse transcriptase PCR (RT-qPCR). The results indicated that both drought and salt stresses altered the expression pattern of miRNAs in a dose-dependent manner. However, each miRNA responded to drought and salt stress in a different pattern among the six sorghum genotypes. miR156, miR167, miR168 and miR399 g ive different expressions levels compared to other studied miRNAs which may attribute to the adaption of sorghum to drought and salt stress and are good candidates for improving sorghum by transgenic technology.
KeywordsmiRNAUp-RegulationDown-RegulationPre-FloweringPost-Flowering
- Carrington, J.C. and Ambros, V. (2003) Role of MicroRNAs in Plant and Animal Development. Science, 301, 336-338. http://dx.doi.org/10.1126/science.1085242
- Bartel, B. and Bartel, D.P. (2003) MicroRNAs: At the Root of Plant Development. Plant Physiology, 132, 709-717. http://dx.doi.org/10.1104/pp.103.023630
- Wang, Y. and Blelloch, R. (2009) Cell Cycle Regulation by MicroRNAs in Embryonic Stem Cells. Cancer Research, 69, 4093-4096. http://dx.doi.org/10.1158/0008-5472.CAN-09-0309
- Sunkar, R., Li, Y.F. and Jagadeeswaran, G. (2012) Functions of MicroRNAs in Plant Stress Responses. Trends in Plant Science, 17, 196-203. http://dx.doi.org/10.1016/j.tplants.2012.01.010
- Hamza, N.B., Elsadig, A.E., Elmunsor, I.I., Ibrahim, A.I.A. and Abuali, A.I. (2016) Assessment of Genetic Diversity in Grain Sorghum (Sorghum bicolor L. Moench) Genotypes under Drought Condition, Using Agro-Morphological Traits and Molecular Markers. International Journal of Plant Breeding and Genetics (Accepted, in Press).
- Li, W.X., Oono, Y., Zhu, J., He, X.J., Wu, J.M., Iida, K., Lu, X.Y., Cui, X., Jin, H. and Zhu, J.K. (2008) The Arabidopsis NFYA5 Transcription Factor Is Regulated Transcriptionally and Post-Transcriptionally to Promote Drought Resistance. Plant Cell, 20, 2238-2251. http://dx.doi.org/10.1105/tpc.108.059444
- Xia, K., Wang, R., Ou, X., Fang, Z., Tian, C., Duan, J., Wang, Y. and Zhang, M. (2012) OsTIR1 and OsAFB2 down Regulation via OsmiR393 over Expression Leads to More Tillers, Early Flowering and Less Tolerance to Salt and Drought in Rice. PLoS ONE, 7, 30-39.
- Zhang, Z.M., Lin, H.J., Shen, Y.O., Gao, J., Xiang, K., Liu, L., Ding, H.P., Yuan, G.S., Lan, H., Zhou, S.F., Zhao, M.J., Gao, S.B., Rong, T.Z. and Pan, G.T. (2012) Cloning and Characterization of miRNAs from Maize Seedling Roots under Low Phosphorus Stress. Molecular Biology Reports, 39, 8137-8146. http://dx.doi.org/10.1007/s11033-012-1661-5
- Doggett, H. (1988) Sorghum. 2nd Edition, Longmans Scientific and Technical, UK.
- Paterson, A.H., et al. (2009) The Sorghum Bicolor Genome and the Diversification of Grasses. Nature, 457, 551-556.
- Du, Z., Zhou, X., Ling, Y., Zhang, Z. and Su, Z. (2010) agriGO: A Goanalysis Toolkit for the Agricultural Community. Nucleic Acids Research, 38, W64-W70. http://dx.doi.org/10.1093/nar/gkq310
- Katiyar, A., Smita, S., Chinnusamy, V., Pandey, D.V. and Bansal, K.C. (2012) Identification of miRNAs in Sorghum by Using Bioinformatics Approach. Plant Signaling and Behavior, 7, 246-259. http://dx.doi.org/10.4161/psb.18914