Bioactive Compounds Produced by <i>Streptomyces</i> sp. Isolate UPMRS4 and Antifungal Activity against <i>Pyricularia oryzae</i> — Oak Academic Publishing
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Bioactive Compounds Produced by <i>Streptomyces</i> sp. Isolate UPMRS4 and Antifungal Activity against <i>Pyricularia oryzae</i>
Department of Plant Protection, Faculty of Agriculture, Universiti Putra Malaysia, Serdang, Malaysia
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Department of Plant Protection, Faculty of Agriculture, Universiti Putra Malaysia, Serdang, Malaysia
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Department of Land Management, Faculty of Agriculture, Universiti Putra Malaysia, Serdang, Malaysia
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Department of Plant Protection, Faculty of Agriculture, Universiti Putra Malaysia, Serdang, Malaysia
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Institute of Tropical Agriculture, Universiti Putra Malaysia, Serdang, Malaysia
1 Department of Plant Protection, Faculty of Agriculture, Universiti Putra Malaysia, Serdang, Malaysia
2 Department of Plant Protection, Faculty of Agriculture, Universiti Putra Malaysia, Serdang, Malaysia
3 Department of Land Management, Faculty of Agriculture, Universiti Putra Malaysia, Serdang, Malaysia
4 Department of Plant Protection, Faculty of Agriculture, Universiti Putra Malaysia, Serdang, Malaysia
5 Institute of Tropical Agriculture, Universiti Putra Malaysia, Serdang, Malaysia
A Streptomyces isolate having antifungal activity against Pyricularia oryzae , the causal agent of rice blast disease, was isolated from soil collected in rice fields of Tanjung Karang Selangor, peninsula Malaysia. The aim of the study was to determine the antifungal activity of Streptomyces sp. isolate UPMRS4 extracts against P. oryzae and to identify bioactive antifungal compounds produced by UPMRS4. Various solvents were used for extraction of antifungal compounds and well diffusion method was used to determine the antifungal activity of the extracts. The ethyl acetate extract demonstrated the highest activity against mycelial growth of P. oryzae , with an effective inhibitory concentration (EIC) of 1.562 μg/ml significantly higher compared to that of chloroform, diethyl ether, methanol, acetone, ethanol and water. Based on GC-MS and LC-MS/MSanalyses, compounds with antifungal activity were detected such as (Pyrrolo[1,2-a] pyrazine-1,4-dione, hexahydro- 3-(2-methylpropyl); Pyrrolo[1,2-a] pyrazine-1,4-dione, hexahydro-3-(phenylmethyl); ergotamine; amicomacin ; fungichromin; rapamycin and N-Acetyl-D, L-phenylalanine . These compounds had good general antifungal activity and might have potential future agricultural applications.
KeywordsGCMSLCMS/MSAntifungal<i>Streptomyces</i>sp. and Rice Blast
Hayasaka, T., Fujii, H. and Ishiguro, K. (2008) The Role of Silicon in Preventing Appressorial Penetration by the Rice Blast Fungus. Phytopathology, 98, 1038-1044. http://dx.doi.org/10.1094/PHYTO-98-9-1038
Berdy, J. (2005) Bioactive Microbial Metabolites. The Journal of Antibiotics, 58, 1-26. http://dx.doi.org/10.1038/ja.2005.1
El-Mehalawy, A.A., Abd-Allah, N.A., Mohammed, R.M. and Abu-Shady, M.R. (2005) Actinomycetes Antagonizing Plant and Human Pathogenic Fungi, II. Factors Affecting Antifungal Production and Chemical Characterization of the Active Components. International Journal of Agriculture and Biology (Pakistan), 7, 188-196.
Demain, A.L. and Sanchez, S. (2009) Microbial Drug Discovery: 80 Years of Progress. The Journal of Antibiotics, 62, 5-16. http://dx.doi.org/10.1038/ja.2008.16
Kavitha, A., Vijayalakshmi, M., Sudhakar, P. and Narasimha, G. (2010) Screening of Actinomycete Strains for the Production of Antifungal Metabolites. African Journal of Microbiology Research, 4, 027-032.
Tiwari, V., Roy, R. and Tiwari, M. (2015) Antimicrobial Active Herbal Compounds against Acinetobacter baumannii and Other Pathogens. Frontiers in Microbiology, 6, 618. http://dx.doi.org/10.3389/fmicb.2015.00618
Snyder, L.R. and Dolan, J.W. (2007) High-Performance Gradient Elution: The Practical Application of the Linear-Solvent-Strength Model. John Wiley & Sons, Hoboken.
Snyder, L.R., Kirkland, J.J. and Glajch, J.L. (2012) Practical HPLC Method Development. John Wiley & Sons, Hoboken.
Villas-Boas, S.G., Mas, S., Akesson, M., Smedsgaard, J. and Nielsen, J. (2005) Mass Spectrometry in Metabolome Analysis. Mass Spectrometry Reviews, 24, 613-646. http://dx.doi.org/10.1002/mas.20032
Huang, H.C., Wang, H.F., Yih, K.H., Chang, L.Z. and Chang, T.M. (2012) Dual Bioactivities of Essential Oil Extracted from the Leaves of Artemisia argyi as an Antimelanogenic versus Antioxidant Agent and Chemical Composition Analysis by GC/MS. International Journal of Molecular Sciences, 13, 14679-14697.
Wang, Y., Chang, L., Zhao, X., Meng, X. and Liu, Y. (2012) Gas Chromatography-Mass Spectrometry Analysis on Compounds in Volatile Oils Extracted from Yuanzhi (Radix polygalae) and Shichangpu (Acorus tatarinowii) by Supercritical CO2. Journal of Traditional Chinese Medicine, 32, 459-464. http://dx.doi.org/10.1016/S0254-6272(13)60055-2
Patel, J.B., Tenover, F.C., Turnidge, J.D. and Jorgensen, J.H. (2011) Susceptibility Test Methods: Dilution and Disk Diffusion Methods. Manual of Clinical Microbiology, 1122-1143. http://dx.doi.org/10.1128/9781555816728.ch68
Viegelmann, C., Margassery, L.M., Kennedy, J., Zhang, T., O'Brien, C., O’Gara, F., and Edrada-Ebel, R. (2014) Metabolomic Profiling and Genomic Study of a Marine Sponge-Associated Streptomyces sp. Marine Drugs, 12, 3323-3351. http://dx.doi.org/10.3390/md12063323
Yuan, W.M. and Crawford, D.L. (1995) Characterization of Streptomyces lydicus WYEC108 as a Potential Biocontrol Agent against Fungal Root and Seed Rots. Applied and Environmental Microbiology, 61, 3119-3128.
Trejo-Estrada, S.R., Sepulveda, I.R. and Crawford, D.L. (1998) In Vitro and in Vivo Antagonism of Streptomyces violaceusniger YCED9 against Fungal Pathogens of Turfgrass. World Journal of Microbiology and Biotechnology, 14, 865-872. http://dx.doi.org/10.1023/A:1008877224089
Sabaratnam, S. and Traquair, J.A. (2002) Formulation of a Streptomyces Biocontrol Agent for the Suppression of Rhizoctonia Damping-Off in Tomato Transplants. Biological Control, 23, 245-253. http://dx.doi.org/10.1006/bcon.2001.1014
Khamna, S., Yokota, A., Peberdy, J.F. and Lumyong, S. (2009) Antifungal Activity of Streptomyces spp. Isolated from Rhizosphere of Thai Medicinal Plants. International Journal of Integrative Biology, 6, 143-147.
Kobayashi, A., Koguchi, Y., Kanzaki, H., Kajiyama, S.I. and Kawazu, K. (1994) A New Type of Antimicrobial Phenolics Produced by Plant Peroxidase and Its Possible Role in the Chemical Defense Systems against Plant Pathogens. Zeitschrift für Naturforschung C, 49, 411-414.
Kumar, R. and Lown, J.W. (2003) Design, Synthesis and in Vitro Cytotoxicity Studies of Novel Pyrrolo [2,1][1,4] Benzodiazepine-Glycosylated Pyrrole and Imidazole Polyamide Conjugates. Organic & Biomolecular Chemistry, 1, 3327-3342. http://dx.doi.org/10.1039/b306685a
Azumi, M., Ogawa, K.I., Fujita, T., Takeshita, M., Yoshida, R., Furumai, T. and Igarashi, Y. (2008) Bacilosarcins A and B, Novel Bioactive Isocoumarins with Unusual Heterocyclic Cores from the marine-derived bacterium Bacillus subtilis. Tetrahedron, 64, 6420-6425. http://dx.doi.org/10.1016/j.tet.2008.04.076
Malinowski, D.P., Belesky, D.P. and Lewis, G.C. (2005) Abiotic Stresses in Endophytic Grasses. In: Roberts, C.A., West, C.P. and Spiers, D.E., Eds., Neotyphodium in Cool-Season Grasses, Blackwell Publishing Ltd., Oxford, 187-199.
Zhang, X., Li, C. and Nan, Z. (2012) Effects of Cadmium Stress on Seed Germination and Seedling Growth of Elymus dahuricus Infected with the Neotyphodium Endophyte. Science China Life Sciences, 55, 793-799. http://dx.doi.org/10.1007/s11427-012-4359-y
Melo, I.S., Santos, S.N., Rosa, L.H., Parma, M.M., Silva, L.J., Queiroz, S.C. and Pellizari, V.H. (2014) Isolation and Biological Activities of an Endophytic Mortierella alpina Strain from the Antarctic Moss Schistidium antarctici. Extremophiles, 18, 15-23. http://dx.doi.org/10.1007/s00792-013-0588-7
Harrison, P.H., Noguchi, H. and Vederas, J.C. (1986) Biosynthesis of Polyene Antibiotics: Intact Incorporation of Carbon-13 Labeled Octanoate into Fungichromin by Streptomyces cellulosae. Journal of the American Chemical Society, 108, 3833-3834. http://dx.doi.org/10.1021/ja00273a050
Baker, H., Sidorowicz, A., Sehgal, S.N. and Vézina, C. (1978) Rapamycin (AY-22,989), a New Antifungal Antibiotic. III. In Vitro and in Vivo Evaluation. The Journal of Antibiotics, 31, 539-545. http://dx.doi.org/10.7164/antibiotics.31.539
Cruz, M.C., Cavallo, L.M., Gorlach, J.M., Cox, G., Perfect, J.R., Cardenas, M.E. and Heitman, J. (1999) Rapamycin Antifungal Action Is Mediated via Conserved Complexes with FKBP12 and TOR Kinase Homologs in Cryptococcus neoformans. Molecular and Cellular Biology, 19, 4101-4112. http://dx.doi.org/10.1128/MCB.19.6.4101
Monaghan, P., Fardis, M., Revill, W.P. and Bell, A. (2005) Antimalarial Effects of Macrolactones Related to FK520 (Ascomycin) Are Independent of the Immunosuppressive Properties of the Compounds. The Journal of Infectious Diseases, 191, 1342-1349. http://dx.doi.org/10.1086/428454
Bastidas, R.J., Shertz, C.A., Lee, S.C., Heitman, J. and Cardenas, M.E. (2012) Rapamycin Exerts Antifungal Activity in Vitro and in Vivo against Mucor circinelloides via FKBP12-Dependent Inhibition of Tor. Eukaryotic Cell, 11, 270-281. http://dx.doi.org/10.1128/EC.05284-11