Plant growth hormone BAP (benzyl amino purine), KIN (kinetin), NAA (1-naphthalene acetic acid) and IBA (indole-3 butyric acid) effect was studied on in vitro multiplication of shoots and rooting of Drepanostachyum falcatum . In vitro micropropagation of himalayan weeping bamboo is explained by in vitro shoot induction and proliferation. Excised explant with axillary bud is surface sterilized with 0.1% HgCl 2 for 10 - 12 minutes, cleaned with 90% ethanol and inoculated on liquid Murashige and Skoog (MS) culture medium supplemented with different concentrations of BAP/ KIN. Effect of BAP/KIN on shoot induction is with different rate and number of shoots produced by explants with axillary bud cultured on MS media supplemented with 0.0 mg/L BAP/KIN - 5.5 mg/L BAP/KIN. Shoot multiplication with highest rate is achieved on MS medium supplemented with 3.5 mg/L BAP after 4 th sub-culturing. The most effective with highest rate and number of root induction combination is 6.5 mg/L IBA after 5 weeks. The roots produced by 6.5 mg/L IBA is best compared with other combination of auxin NAA (1-naphthalene acetic acid).
Kapur, S.K. (1991) Economically Useful Plants of Majauri-Kirchi Forest Tract (Jammu and Kashmir). Journal of Economic and Taxonomic Botany, 14, 534.
Uniyal, S. and Awasthi, A. (2000) Bamboos: Their Distribution and Biomass in Bhagirathi Catchment, Garhwal Himalaya. Indian Journal of Forestry, 4, 490-495.
Naithani, H.B. and Chandra, S. (1998) Gregarious Flowering of a Bamboo (Drepanostachyum falcatum). Indian Forester, 8, 663-666.
Kondas, S. (1982) Bamboo Biology, Culm Potential and Problems of Cultivation. Indian Forester, 3, 179-188.
Rao, I.V.R. and Rao, I.U. (1988) In: Bamboo current Research. In: Rao, I.V.R., Gnanaharan, R. and Cherla, B.S. (Eds.), Proc. International Bamboo Workshop, FRI, Kerala & IDRC, Canada, 151.
Arya, I.D. and Arya, S. (1996) Introduction, Mass Multiplication and Establishment of Edible Bamboo Dendrocalamus asper in India. Indian Journal of Plant Genetic Resources, 1, 115-121.
Arya, I.D. and Arya, S. (1997) In Vitro Culture and Establishment of Exotic Bamboo Dendrocalamus asper. Indian Journal of Experimental Biology, 35, 1252-1255.
Murashige, T. and Skoog, F. (1962) A Revised Medium for Rapid Growth and Bio Assay with Tobacco Tissue Culture. Physiologia Plantarum, 15, 473-497. http://dx.doi.org/10.1111/j.1399-3054.1962.tb08052.x
Das, P. and Rout, G.R. (1994) Analysis of Current Methods and Approaches on the Micropropagation of Bamboo. Biological Sciences, 3, 235-246.
Arya, S. and Sharma, S. (1998) Micropropagation Technology of Bambusa bambos through Shoot Proliferation. Indian Forester, 9, 725-731.
Ramanayke, S.M.S.D., Wanniarachchi, W.A.V.R. and Tennakoon, T.M.A. (2001) Axillary Shoot Proliferation and in Vitro Flowering in an Adult Giant Bamboo, Dendrocalamus giganteus Wall. Ex Munro. In Vitro Cellular & Developmental Biology-Plant, 37, 667-671. http://dx.doi.org/10.1007/s11627-001-0116-9
Arya, S., Satsangi, R. and Arya, I.D. (2002) Rapid Mass Multiplication of Edible Bamboo Dendrocalamus asper. Journal of Sustainable Forestry, 14, 103-114. http://dx.doi.org/10.1300/J091v14n02_06
Arya, S., Rana, P.K., Sharma, R. and Arya, I.D. (2006) Tissue Culture Technology for Rapid Multiplication of Dendrocalamus giganteus Munro. Indian Forester, 3, 345-357.
Ramanayke, S.M.S.D. and Yakandwala, K. (1997) Micropropagation of the Giant Bamboo (Dendrocalamus giganteus (Munro) from Nodal Explants of Field-Grown Culm. Plant Science, 129, 213-223. http://dx.doi.org/10.1016/S0168-9452(97)00185-4
Hirimburegama, K. and Gamage, N. (1995) Propagation of Bambusa vulgaris (Yellow Bamboo) through Nodal Bud Culture. The Journal of Horticultural Science and Biotechnology, 3, 469-475. http://dx.doi.org/10.1080/14620316.1995.11515317
Huang, L.C. and Huang, B.L. (1995) Loss of the Species Distinguishing Trait among Regenerated Bambusa ventricosa McClure Plants. Plant Cell, Tissue and Organ Culture, 42, 109-111. http://dx.doi.org/10.1007/BF00037688
Prutpongse, P. and Gavinlertvatana, P. (1992) In Vitro Micropropagation of 54 Species from 15 Genera of Bamboo. Horticultural Science, 27, 453-454.
Bag, N., Chandra, S., Palni, L.M.S. and Nandi, S.K. (2000) Micropropagation of Dev-Ringal [Thamnocalamus spathiflorus (Trin.) Munro]—A Temperate Bamboo, and Comparison between in Vitro Propagated Plants and Seedlings. Plant Science, 156, 125-135. http://dx.doi.org/10.1016/S0168-9452(00)00212-0
Saxena, S. (1990) In Vitro Propagation of Bamboo (Bambusa tulda Roxb.) through Shoot Propagation. Plant Cell Reports, 9, 431-434. http://dx.doi.org/10.1007/BF00232266
Sood, A., Sharma, O.P. and Palni, L.M.S. (1992) Improved Methods of Propagation of Maggar Bamboo. (Dendrocalamus hamiltonii Nees et Arn. ex Munro) Using Single Node Cutting Taken from Juvenile Culms of Elite Seedlings. The Journal of the American Bamboo Society, 182, 17-24.
Mrudul, V., Shirgurkar, S., Thengane, R., Insiya, S., Poonawala, J., Nadgauda, R.S. and Mascarenhas, A.F. (1996) A Simple in Vitro Method of Propagation and Rhizome Formation in Dendrocalamus strictus Nees. Current Science, 10, 940-943.
Chambers, S.M., Heuch, J.H.R. and Pirrie, A. (1991) Micropropagation and in Vitro Flowering of the Bamboo Dendrocalamus hamiltonii Munro. Plant Cell, Tissue and Organ Culture, 27, 45-48. http://dx.doi.org/10.1007/BF00048205
Mudoi, K.D. and Borthakur, M. (2009) In Vitro Micropropagation of Bambusa balcooa Roxb. through Nodal Explants from Field Grown Culms and Scope for Upscaling. Current Science, 7, 962-966. http://www.ias.ac.in/currsci
Negi, D. and Saxena, S. (2011) Micropropagation of Bambusa balcooa Roxb. through Axillary Shoot Proliferation. In Vitro Cellular & Developmental Biology—Plant, 5, 604-610. http://dx.doi.org/10.1007/s11627-011-9403-2
Arya, I.D., Kaur, B. and Arya, S. (2012) Rapid and Mass Propagation of Economically Important Bamboo Dendrocalamus hamiltonii. Indian Journal of Energy, 1, 11-16.
Mudoi, K.D., Saikia, S.P., Goswami, A., Gogoi, A., Bora, D. and Borthakur, M. (2013) Micropropagation of Important Bamboos: A Review. African Journal of Biotechnology, 20, 2770-2785. http://www.academicjournals.org/AJB
Waikhom, S.D. and Louis, B. (2014) An Effective Protocol for Micropropagation of Edible Bamboo Species Bambusa tulda and Melocanna baccifera through Nodal Culture. The Scientific World Journal, 2014, Article ID: 345794. http://dx.doi.org/10.1155/2014/345794
Nadgir, A.L., Phadke, C.H., Gupta, P.K., Parasharami, V.A., Nair, S. and Mascarenhas, A.F. (1984) Rapid Multiplication of Bamboo by Tissue Culture. Silvae Genetica, 6, 219-233.
Bonga, J.M. and Von Aderkas, P. (1992) In Vitro Culture of Trees. Kluwer Academic Publishers, Dordrecht, 43. http://dx.doi.org/10.1007/978-94-015-8058-8
Bhati, R., Shekhawat, N.S. and Arya, H.C. (1992) In Vitro Regeneration of Plantlets from Root Segments of Aegle marmelos. Indian Journal of American Biology, 844-845.
Tricoli, D.M., Maynard, C.A. and Drew, A.P. (1985) Tissue Culture Propagation of Mature Trees of Prunus serotina Ehrh. I. Establishment, Multiplication and Rooting in Vitro. Forest Science, 31, 201-208.
Yadav, U., Lal., M. and Jaiswal, V.S. (1990) In Vitro Micropropagation of the Tropical Fruit Tree Syzygium cuminii L. Plant Cell, Tissue and Organ Culture, 21, 87-92. http://dx.doi.org/10.1007/BF00034498
Yeh, M.L. and Chang, W.C. (1986) Plant Regeneration through Somatic Embryogenesis in Callus Culture of Green Bamboo (Bambusa oldhamii). Theoretical and Applied Genetics, 73, 161-163. http://dx.doi.org/10.1007/BF00289269
Yeh, M.L. and Chang, W.C. (1987) Plant Regeneration via Somatic Embryogenesis in Mature Embryo Derived Callus Cultures of Sinocalamus latiflora (Munro) McClure. Plant Science, 51, 93-96. http://dx.doi.org/10.1016/0168-9452(87)90224-X
Huang, L.C., Huang, B.L. and Chang, W.L. (1989) Tissue Culture Investigations of Bamboo—IV. Organogenesis Leading to Adventitious Shoots and Plants in Excised Shoot Apices. Environmental and Experimental Botany, 29, 307-315. http://dx.doi.org/10.1016/0098-8472(89)90004-X
Ramanayke, S.M.S.D. and Wanniarachchi, W.A.V.R. (2003) Organogenesis in Callus Derived from an Adult Giant Bamboo (Dendrocalamus giganteus Wall. ex Munro). Scientia Horticulturae, 98, 195-200. http://dx.doi.org/10.1016/S0304-4238(02)00204-2
Yashodha, R., Sumathi, R., Mallinga, P. and Gurumurthi, K. (1997) Genetic Enhancement and Mass Production of Quality Propagules of Bambusa nutans and Dendrocalamus membranaceus. Indian Forester, 4, 303-306.
Rathore, S. and Ravishankar Rai, V. (2005) Micropropagation of Pseudoxytenanthera stocksii Munro. In Vitro Cellular & Developmental Biology—Plant, 3, 333-337.
Arya, S., Sharma, S., Kaur, R. and Arya, I.D. (1999) Micropropagation of Dendrocalamus asper by Shoot Proliferation Using Seeds. Plant Cell Reports, 18, 879-882. http://dx.doi.org/10.1007/s002990050678