<i>In-Vitro</i> Production of Anthocyanin in <i>Sesbania grandiflora</i> (Red Katuray) as Influenced by Varying Concentrations of 2,4-D and BA Added on MS Medium

Abstract

The study aimed to determine the anthocyanin content in callus cultures of Sesbania grandiflora L. (Red Katuray) using petal explants as influenced by 2,4-D and BA. The specific objective of this study is to determine the effect of plant growth regulators specifically auxin (2,4-D) and BA (Benzyladenine) on Murashige and Skoog’s (MS) medium in anthocyanin production. Earliest callus initiation was noted in 2.5 ppm to 5.0 ppm 2,4-D without BA and 2.5 ppm 2,4-D with 2.5 ppm BA. Same treatment combination had the highest degree of callus formation and obtained the greatest percentage of callus formation 2.5 ppm 2,4-D without BA and 2.5 ppm 2,4-D with 2.5 ppm BA. The heaviest weight of callus was registered at 2.5 ppm 2,4-D without BA. Significantly higher anthocyanin content was noted in 2.5 ppm to 5.0 ppm 2,4-D without or with 2.5 ppm BA, with 2.5 and 5.0 ppm BA and with 5.0 ppm 2,4-D and 2.5 ppm BA. There is a positive relationship between callus formation and anthocyanin production. Results showed that treatments that promoted significantly greater callus formation e.g. 2.5 ppm 2,4-D alone or in combination with 2.5 ppm BA also produced the highest anthocyanin content.