Assessing Genetic Variation and Relationships among a Mini Core Germplasm of Sesame (<i>Sesamum indicum</i> L.) Using Biochemical and RAPD Markers — Oak Academic Publishing
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Assessing Genetic Variation and Relationships among a Mini Core Germplasm of Sesame (<i>Sesamum indicum</i> L.) Using Biochemical and RAPD Markers
Faculty of Agronomic Sciences, University of Abomey-Calavi, Abomey-Calavi, Benin
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Department of Agronomy, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, China
1 Faculty of Agronomic Sciences, University of Abomey-Calavi, Abomey-Calavi, Benin
2 Department of Agronomy, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, China
Knowledge of genetic diversity is essential for any future varietal improvement program. Thus, the objective of this study is to contribute to a better knowledge of the genetic diversity within 15 sesame accessions, using biochemical markers and RAPD. The accessions are of various sources and seeds of different colors; color being an important feature in the sesame market. SDS-PAGE on protein extracts of sesame seeds revealed 31 protein markers, only 4 of which were polymorphic, indicating that this technique is not suitable for studying genetic diversity in S. indicum . The analyses on isoenzymatic extracts of esterase from sesame seedlings made it possible, to distinguish and classify the accessions into 2 major groups. RAPD analysis of genomic DNA using 10-mer primers revealed a high inter-accession genetic diversity. A total of 53 DNA markers, including 48 polymorphic, were produced. The coefficient of similarity between accessions ranged from 0.343 to 0.897. RAPD markers were found to be more suitable for studying genetic diversity within sesame germplasm than isozyme analyzes. Nevertheless, geographical distributions and seed colors are not related to the classes formed.
Anilakumar, K.R., Pal, A., Khanum, F. and Bawa, A.S. (2010) Nutritional, Medicinal and Industrial Uses of Sesame (Sesamum indicum L.) Seeds—An Overview. Agriculturae Conspectus Scientificus, 75, 159-168.
FAOSTAT (2013) Food and Agriculture Organization of the United Nations (FAO). Rome.
Pathak, N., Rai, A.K., Kumari, R., Thapa, A. and Bhat, K.V. (2014) Sesame Crop: An Underexploited Oilseed Holds Tremendous Potential for Enhanced Food Value. Agricultural Sciences, 5, 519-529. https://doi.org/10.4236/as.2014.56054
Lakhanpaul, S., Singh, V., Kumar, S., Bhardwaj, D., Bhat, K.V., Tuteja, N., Gill, S.S., Tiburcio, A.F. and Tuteja, R. (2012) Sesame: Overcoming the Abiotic Stresses in the Queen of Oilseed Crops in Improving Crop Resistance to Abiotic Stress. In: Tuteja, N., Singh, S., Tiburcio, A.F. and Tuteja, R., Eds., Improving Crop Resistance to Abiotic Stress, Vol. I and Vol. II, John Wiley & Sons, Hoboken, 1251-1283.
Silme, R.S. and Cagirgan, M.I. (2010) Screening for Resistance to Fusarium Wilt in Induced Mutants and World Collection of Sesame under Intensive Management. Turkish Journal of Field Crops, 15, 89-93.
Alege, G.O., Akinyele, B.O., Ayodele, S.M. and Ogbode, A.V. (2011) Taxonomic Importance of the Vegetative and Pod Characteristics in Three Nigerian Species of Sesame. African Journal of Plant Science, 5, 213-217.
Ainsworth, E.A. and Ort, D.R. (2010) How Do We Improve Crop Production in a Warming World? Plant Physiology, 154, 526-530. https://doi.org/10.1104/pp.110.161349
Zhang, X.R., Zhao, Y.Z., Feng, X.Y., Cheng, Y., Guo, Q.Y., Li, Y.R. and Wen Y.N. (1999) Establishment and Development of Sesame Germplasm Core Collection in China. Plant Genetic Resources Newsletter, 119, 47-50.
Bisht, I.S, Mahajan, R.K., Loknathan, T.R., Gautam, P.L., Mathur, P.N. and Hodgkin, T. (1999) Assessment of Genetic Diversity, Stratification of Germplasm Accessions in Diversity Groups and Sampling Strategies for Establishing a Core Collection of Indian Sesame (Sesamum indicum L.). Plant Genetic Resources Newsletter, 119, 35-46.
Feng, X.Y. (1999) Research on Sesame Germplasm of China. Chinese Journal of Oil crop Science, 21, 77-80.
Azeez, M.A., Aremu, C. and Olaniyan, O.O. (2013) Assessment of Genetic Variation in Accessions of Sesame (Sesamum indicum L.) and Its Crosses by Seed Protein Electrophoresis. Journal of Agroalimentary Processes and Technologies, 19, 383-391.
Zhao, Y.Z., Cheng, Y., Zhang, X.R, Feng, X.Y., Fu, M., Guo, Q.Y., Zhou, M.D. and Hodgking, T. (1998) Isozyme Analysis of Sesame Core Collection and Reserve Collection. Chinese Journal of Oil Crop Sciences, 20, 29-34.
Nanthakumar, G., Singh, K.N. and Vaidyanathan, P. (2000) Relationships between Cultivated Sesame (Sesamum sp.) and the Wild Relatives Based on Morphological Characters, Isozymes and RAPD Markers. Journal of Genetics and Breeding, 54, 5-12.
Sarkar, S., Poddar, R., Basu, D., Basu, T. and Saha, P.K. (2012) Optimization of RAPD Method and Its Application for the Analysis of Genetic Variability in Cultivated and Wild Indian Sesame. Indian Journal of Science and Research, 3, 47-54.
Tabatabaei, I., Pazouki, L., Bihamta, M.R., Mansoori, S., JalaliJavaran, M. and Niinemets, ü. (2011) Genetic Variation among Iranian Sesame (Sesamum indicum L.) Accessions vis-à-vis Exotic Genotypes on the Basis of Morpho-Physiological Traits and RAPD Markers. Australian Journal of Crop Science, 5, 1396-1407.
Wei, X., Wang, L., Zhang, Y., Qi, X., Wang, X., Ding, X., Zhang, J. and Zhang, X, (2014) Development of Simple Sequence Repeat (SSR) Markers of Sesame (Sesamum indicum) from a Genome Survey. Molecules, 19, 5150-5162. https://doi.org/10.3390/molecules19045150
Admas, A., Yohannes, P. and Kassahun, T. (2013) Genetic Distance of Sesame (Sesamum indicum L.) Cultivars and Varieties from Northwestern Ethiopia Using Inter Simple Sequence Repeat Markers. East African Journal of Sciences, 7, 31-40.
Adéoti, K., Rival, A., Dansi, A., Santoni, S., Brown, S., Beule, T., Nato, A., Henry, Y., Vodouhe, R., Loko, L.Y. and Sanni, A. (2011) Genetic Characterization of Two Traditional Leafy Vegetables (Sesamumradiatum Thonn. ex Hornem and Ceratothecasesamoides Endl.) of Benin, Using Flow Cytometry and Amplified Fragment Length Polymorphism (AFLP) Markers. African Journal of Biotechnology, 10, 14264-14275. https://doi.org/10.5897/AJB11.1176
Zhang, Y.X., Zhang, X.R., Hua, W., Wang, L.H. and Che, Z. (2010) Analysis of Genetic Diversity among Indigenous Landraces from Sesame (Sesamum indicum L.) Core Collection in China as Revealed by SRAP and SSR Makers. Genes Genomics, 32, 207-215. https://doi.org/10.1007/s13258-009-0888-6
Williams, J.G.K., Kubelik, A.R., Livak, K.J., Rafalski, J.A. and Tingey, S.S. (1990). DNA Polymorphism Amplified Primers Are Useful as Genetic Markers. Nucleic Acids Research, 18, 6531-6535. https://doi.org/10.1093/nar/18.22.6531
ISTA (1996) International Rules for Seed Testing 1996. The International Seed Testing Association, Zurich.
Nei, M. and Li, W.H. (1979) Mathematical Model for Studying Variation in Terms of Restriction Endonucleases. Proceedings of the National Academy of Sciences, 76, 5269-5273. https://doi.org/10.1073/pnas.76.10.5269
Rohlf, F.J. (2005) NTSYS-PC: Numerical Taxonomy and Multivariate Analysis System, Version 2.2. Exeter Software, Setauket.
Zhang, H., Miao, H., Wei, L., Li, C., Zhao, R., et al. (2013) Genetic Analysis and QTLMapping of Seed Coat Color in Sesame (Sesamum indicum L.). PLoS ONE, 8, e63898. https://doi.org/10.1371/journal.pone.0063898
Laurentin, H. and Benítez, T. (2014) Inheritance of Seed Coat Color in Sesame. Pesquisa Agropecuária Brasileira, 49, 290-295. https://doi.org/10.1590/S0100-204X2014000400007
Ashri, A. (1998) Sesame Breeding. In: Janick, J., Ed., Plant Breeding Reviews, John Wiley & Sons, Inc., Hoboken, 179-228. https://doi.org/10.1002/9780470650110.ch5
Bhat, K.V., Babrekar, P.P. and Lakhanpaul, S. (1999) Study of Genetic Diversity in Indian and Exotic Sesame (Sesamum indicum L.) Germplasm Using Random Amplified Polymorphic DNA (RAPD) Markers. Euphytica, 110, 21-33. https://doi.org/10.1023/A:1003724732323
Ercan, A.G., Taskin, M. and Turgut, K. (2004) Analysis of Genetic Diversity in Turkish Sesame (Sesamum indicum L.) Populations Using RAPD Markers. Genetic Resources and Crop Evolution, 51, 599-607. https://doi.org/10.1023/B:GRES.0000024651.45623.f2
Kim, D.H., Zur, G., Danin-Poleg, Y., Lee, S.W., Shim, K.B., Kang, C.W. and Kashi, Y. (2002) Genetic Relationships of Sesame Germplasm Collection as Revealed by Inter-Simple Sequence Repeats. Plant Breeding, 121, 259-262. https://doi.org/10.1046/j.1439-0523.2002.00700.x
Pham, T.D. (2011) Analyses of Genetic Diversity and Desirable Traits in Sesame (Sesamum indicum L., Pedaliaceae): Implication for Breeding and Conservation. Doctoral Thesis, Swedish University of Agricultural Sciences, Lomma Municipality.
Quenum Florent, J.-B., Yan, Q. and Gong, Q. (2004) Phenotypic Variation and Relationships among Sesame (Sesamum indicum L.) Sub-Core Collections. Journal of Zhejiang University (Agriculture & Life Sciences), 30, 10-16.
Fazal, A., Nahida, Y., Ashiq, R.M., Zabta, K.S. and Shahid, M.M. (2012) Study of Total Seed Proteins Pattern of Sesame (Sesamum indicum L.) Landraces via Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis (SDS-PAGE). Pakistan Journal of Botany, 44, 2009-2014.
Kakaei, M. and Kahrizi, D. (2011) Evaluation of Seed Storage Protein Patterns of Ten Wheat Varieties Using SDS-PAGE. Biharean Biologist, 5, 116-118.
Choudhary, R., Rai, G.K.,. Rai, S.K, Parveen, A., Rai, P.K. and Salgotra, R.K. (2015) Genetic Diversity of Brassica napus Using Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis (SDS-PAGE). SABRAO Journal of Breeding and Genetics, 47, 14-20.
Quenum, F.J.-B. and Yan, Q.C. (2001) Differences in Ultradry Seed Storage Potential of Sesame Accessions. Chinese Journal of Oil Crop Sciences, 23, 76-78.
Khlestkina, E.K., Huang X.Q., Quenum F.J.-B., Chebotar S., Roder M.S. and Borner, A. (2004) Genetic Diversity in Cultivated Plants-Loss or Stability? Theoretical and Applied Genetics, 108, 1466-1472. https://doi.org/10.1007/s00122-003-1572-x
Leelambika, M., Mahesh, S., Mahammad, J. and Sathyanarayana, N. (2010) Comparative Evaluation of Genetic Diversity among Mucuna Species Using Morphometric, Biochemical and Molecular Approaches. World Journal of Agricultural Sciences, 6, 568-578.
Laurentin, H.E. and Petr, K. (2006) Genetic Relationship and Diversity in a Sesame (Sesamum indicum L.) Germplasm Collection Using Amplified Fragment Length Polymorphism (AFLP). BMC Genetics, 7, 10. https://doi.org/10.1186/1471-2156-7-10