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Expression Vector Construction and Genetic Transformation of <i>Rosa rugosa β</i>-l,3-Glucanase Gene (<i>RrGlu</i>)
College of Horticulture Science and Engineering, Shandong Agricultural University, Tai’an, China
Forestry College of Shandong Agricultural University, Tai’an, China
Tai’an Landscape Administration Bureau, Tai’an, China
Forestry College of Shandong Agricultural University, Tai’an, China
Forestry College of Shandong Agricultural University, Tai’an, China
Forestry College of Shandong Agricultural University, Tai’an, China
Forestry College of Shandong Agricultural University, Tai’an, China
- 1 College of Horticulture Science and Engineering, Shandong Agricultural University, Tai’an, China
- 2 Forestry College of Shandong Agricultural University, Tai’an, China
- 3 Tai’an Landscape Administration Bureau, Tai’an, China
- 4 Forestry College of Shandong Agricultural University, Tai’an, China
- 5 Forestry College of Shandong Agricultural University, Tai’an, China
- 6 Forestry College of Shandong Agricultural University, Tai’an, China
- 7 Forestry College of Shandong Agricultural University, Tai’an, China
American Journal of Plant Sciences·Volume 08 (2017)·Pages 495–501·Published 4 February 2017·DOI10.4236/ajps.2017.83034
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Abstract
In order to lay a foundation for researching the function of Rosa rugose ( R. rugosa ) RrGlu gene, the RrGlu gene was amplified from the styles of R. rugosa “Tanghong”, a gene expression vector named PBI121- RrGlu was constructed and the vector was introduced into tobacco with the agrobacterium-mediated method. PCR results showed that the RrGlu gene was integrated into the tobacco genome.
Keywords<i>Rosa rugoseβ</i>-l3-Glucanase GeneExpression Vector ConstructionGenetic Transformation
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