Endopolygalacturonase (endoPG) plays a pivotal role in determining peach [ Prunus persica L. (Batsch)] fruit characteristics. Different Pp-endoPG genes or allelic variants have been described, characterized by different polymorphisms: insertions-deletions (InDels) and single nucleotide polymorphisms (SNPs). Eighty-five peach accessions (comprising commercial cultivars, F 1 progenies of selected crosses, and three haploid seedlings) with different flesh softening patterns (Non Melting: NM; Melting: M; Slow Softening: SS; Stony Hard: SH) were screened by exploiting specific polymorphisms, with the aim to characterize their asset at the endoPG locus and evaluate a potential relationship with fruit flesh texture phenotype. The results of InDel analysis allowed to distinguish, by a simple genotyping procedure, NM flesh phenotypes from the others . Further information arose from this analysis, showing that two Pp-endoPG genes, i.e. , Pp-endoPG m ( Ppa006839m ), involved in the determination of the Melting/Non Melting trait , and Pp-endoPG_M ( Ppa006857m ), involved in the determination of the Clingstone/Freestone trait, always co-segregate, and th at SS Big Top possesses a “null” Pp-endoPG allele. Cleaved Amplified Polymorphic Sequence (CAPS) analysis allowed to preliminarily discriminate the Pp-endoPG variants of the SS and SH accessions considered. The integrated use of the considered polymorphisms in a high number of peach accessions proved useful, by individuating the different gene variants and their combinations, to describe the structure of the endoPG locus in different genotypes.
Bruhn, C.M. (1995) Consumer and Retailer Satisfaction with the Quality and Size of California Peaches and Nectarines. Journal of Food Quality, 18, 241-256. https://doi.org/10.1111/j.1745-4557.1995.tb00378.x
Bassi, D. and Monet, R. (2008) 1. Botany and Taxonomy. In: Layne, D.R. and Bassi, D., Eds., The Peach: Botany, Production and Uses, CAB International, Wallingford, 1-36. https://doi.org/10.1079/9781845933869.0001
Yoshida, M. (1976) Genetic Studies on the Fruit Quality of Peach Varieties. III. Texture and Keeping Quality. Bulletin of the Fruit Tree Research Station, 3, 1-16. (In Japanese with English Abstract)
Peace, C.P., Crisosto, C.H. and Gradziel, T.M. (2005) Endopolygalacturonase: A Candidate Gene for Freestone and Melting Flesh in Peach. Molecular Breeding, 16, 21-31. https://doi.org/10.1007/s11032-005-0828-3
Morgutti, S., Negrini, N., Nocito, F.F., Ghiani, A., Bassi, D. and Cocucci, M. (2006) Changes in Endopolygalacturonase Levels and Characterization of a Putative Endo-PG Gene During Fruit Softening in Peach Genotypes with Nonmelting and Melting Flesh Fruit Phenotypes. New Phytologist, 171, 315-328. https://doi.org/10.1111/j.1469-8137.2006.01763.x
Ghiani, A., Negrini, N., Morgutti, S., Baldin, F., Nocito, F.F., Spinardi, A., et al. (2011) Melting of “Big Top” Nectarine Fruit: Some Physiological, Biochemical, and Molecular Aspects. Journal of the American Society for Horticultural Science, 136, 61-68.
Byrne, D.H., Raseira, M.B., Bassi, D., Piagnani, M.C., Gasic, K., Reighard, G.L., et al. (2012) Peach. In: Badenes, M.L. and Byrne, D.H., Eds., Fruit Breeding, Series Handbook of Plant Breeding 8, Springer, New York, 505-569. https://doi.org/10.1007/978-1-4419-0763-9_14
Redgwell, R.J. and Fischer, M. (2002) 3. Fruit Texture, Cell Wall Metabolism and Consumer Perceptions. In: Knee, M., Ed., Fruit Quality and Its Biological Basis, Sheffield Academic Press, Sheffield, 46-88.
Negi, P.S. and Kanda, A.K (2008) 8. Structural Deterioration of the Produce: The Breakdown of Cell Wall Components. In: Paliyath, G., Murr, D.P., Handa, A.K. and Lurie, S., Eds., Postharvest Biology and Technology of Fruits, Vegetables, and Flowers, Wiley-Blackwell, Ames, 162-194.
Lester, D.R., Speirs, G., Orr, G. and Brady, C.J. (1994) Peach (Prunus persica) Endo-PG cDNA Isolation and mRNA Analysis in Melting and Non-Melting Peach Cultivars. Plant Physiology, 105, 225-231. https://doi.org/10.1104/pp.105.1.225
Lester, D.R., Sherman, W.B. and Atwell, B.J. (1996) Endopolygalacturonase and the Melting Flesh (M) Locus in Peach. Journal of the American Society for Horticultural Science, 121, 231-235.
Callahan, A.M., Scorza, R., Bassett, C., Nickerson, M. and Abeles, F.B. (2004) Deletions in an Endopolygalacturonase Gene Cluster Correlate with Non-Melting Flesh Texture in Peach. Functional Plant Biology, 31, 159-168. https://doi.org/10.1071/FP03131
Peace, C.P., Callahan, A., Ogundiwin, E.A., Potter, D., Gradziel, T.M., Bliss, F.A., et al. (2007) Endopolygalacturonase Genotypic Variation in Prunus. In: Litz, R.E. and Scorza, R., Eds., International Symposium on Biotechnology of Temperate Fruit Crops and Tropical Species, Acta Horticulturae, 738, 639-646. https://doi.org/10.17660/actahortic.2007.738.83
Zou, X., Shi, C., Austin, R.S., Merico, D., Munholland, S., Marsolais, F., et al. (2014) Genome-Wide Single Nucleotide Polymorphism and Insertion-Deletion Discovery through Next-Generation Sequencing of Reduced Representation Libraries in Common Bean. Molecular Breeding, 33, 769-778. https://doi.org/10.1007/s11032-013-9997-7
Agarwal, M., Shrivastava, N. and Padh, H. (2008) Advances in Molecular Marker Techniques and their Applications in Plant Science. Plant Cell Reports, 27, 617-631. https://doi.org/10.1007/s00299-008-0507-z
Chavez, D.J., Beckman, T.G., Werner, D.J. and Chaparro, J.X. (2014) Genetic Diversity in Peach (Prunus persica (L.) Batsch) at the University of Florida: Past, Present and Future. Tree Genetics & Genomes, 10, 1399-1417. https://doi.org/10.1007/s11295-014-0769-2
Salazar, J.A., Ruiz, D., Campoy, J.A., Sánchez-Pérez, R., Crisosto, C.H., Martínez-García, P.J., et al. (2014) Quantitative Trait Loci (QTL) and Mendelian Trait Loci (MTL) Analysis in Prunus: A Breeding Perspective and Beyond. Plant Molecular Biology Reporter, 32, 1-18. https://doi.org/10.1007/s11105-013-0643-7
Verde, I., Abbott, A.G., Scalabrin, S., Jung, S., Su, S., Marroni, F., et al. (2013) The High-Quality Draft Genome of Peach (Prunus persica) Identifies Unique Patterns of Genetic Diversity, Domestication and Genome Evolution. Nature Genetics, 45, 487-494. https://doi.org/10.1038/ng.2586
Ahmad, R., Parfitt, D.E., Fass, J., Ogundiwin, E., Dhingra, A. and Crisosto, C.H. (2011) Whole Genome Sequencing of Peach (Prunus persica L.) for SNP Identification and Selection. BMC Genomics, 12, 569. https://doi.org/10.1186/1471-2164-12-569
Verde, I., Bassil, N., Scalabrin, S., Gilmore, B., Lawley, C.T., Gasic, K., et al. (2012) Development and Evaluation of a 9K SNP Array for Peach by Internationally Coordinated SNP Detection and Validation in Breeding Germplasm. PLoS ONE, 7, e35668. https://doi.org/10.1371/annotation/33f1ba92-c304-4757-91aa-555de64a0768
Frett, T.J., Reighard, G.L., Okie, W.R. and Gasic, K. (2014) Mapping Quantitative Trait Loci Associated with Blush in Peach (Prunus persica (L.) Batsch). Tree Genetics & Genomes, 10, 367-381. https://doi.org/10.1007/s11295-013-0692-y
Arús, P., Verde, I., Sosinski, B., Zhebentyayeva, T. and Abbott, A.G. (2012) The Peach Genome. Tree Genetics & Genomes, 8, 531-547. https://doi.org/10.1007/s11295-012-0493-8
Gu, C., Wang, L., Wang, W., Zhou, H., Ma, B., Zheng, H., et al. (2016) Copy Number Variation of a Gene Cluster Encoding Endopolygalacturonase Mediates Flesh Texture and Stone Adhesion in Peach. Journal of Experimental Botany, 67, 1993-2005. https://doi.org/10.1093/jxb/erw021
Sherman, W.B., Topp, B.L. and Lyrene, P.M. (1990) Non-Melting Flesh for Fresh Market Peaches. Proceedings of the Florida State Horticultural Society, 103, 293-294.
Crisosto, C.H. and Valero, D. (2008) 22. Harvesting and Postharvest Handling of Peaches for the Fresh Market. In: Layne, D.R. and Bassi, D., Eds., The Peach: Botany, Production and Uses, CAB International, Wallingford, 575-596. https://doi.org/10.1079/9781845933869.0575
Slaughter, D.C., Crisosto, C.H., Hasey, J.K. and Thompson, J.F. (2006) Comparison of Instrumental and Manual Inspection of Clingstone Peaches. Applied Engineering in Agriculture, 22, 883-889. https://doi.org/10.13031/2013.22242
Lukowitz, W., Gillmor, C.S. and Scheible, W. (2000) Positional Cloning in Arabidopsis. Why It Feels Good to Have a Genome Initiative Working for You. Plant Physiology, 123, 795-806. https://doi.org/10.1104/pp.123.3.795
Geuna, F., Maitti, C., Digiuni, S. and Banfi, R. (2004) A Method for Extracting Genomic DNA Suitable for Medium-Throughput Applications from Plant Tissues Rich in Contaminants. Plant Molecular Biology Reporter, 22, 87. https://doi.org/10.1007/BF02773354
Warburton, M.L. and Bliss, F.A. (1996) Genetic Diversity in Peach (Prunus persica L. Batsch) Revealed by Randomly Amplified Polymorphic DNA (RAPD) Markers and Compared to Inbreeding Coefficients. Journal of the American Society for Horticultural Science, 121, 1012-1019.
Iezzoni, A., Weebadde, C., Luby, J., Chengyan, Y., van de Weg, E., Fazio, G., et al. (2010) RosBREED: Enabling Marker-Assisted Breeding in Rosaceae. Acta Horticulturae, 859, 389-394. http://www.actahort.org/books/859/859_47.htm https://doi.org/10.17660/ActaHortic.2010.859.47
RosBREED (2016) Ppe-Texture: DNA Tests for Peach Texture. http://www.rosbreed.org/breeding/dna-tests/peach/texture
Tatsuki, M., Haji, T. and Yamaguchi, M. (2006) The Involvement of 1-Aminocyclopropane-1-Carboxylic Acid Synthase Isogene, Pp-ACS1, in Peach Fruit Softening. Journal of Experimental Botany, 57, 1281-1289. https://doi.org/10.1093/jxb/erj097
Hayama, H., Tatsuki, M., Ito, A. and Kashimura, Y. (2006) Ethylene and Fruit Softening in the Stony Hard Mutation in Peach. Postharvest Biology and Technology, 41, 16-21. https://doi.org/10.1016/j.postharvbio.2006.03.006
Pan, L., Zeng, W., Niu, L., Lu, Z., Liu, H., Cui, G., et al. (2015) PpYUC11, a Strong Candidate Gene for the Stony Hard Phenotype in Peach (Prunus persica L. Batsch), Participates in IAA Biosynthesis during Fruit Ripening. Journal of Experimental Botany, 66, 7031-7044. https://doi.org/10.1093/jxb/erv400
Okie, W.R. and Layne, D.R. (2008) “Scarletprince” and “Julyprince” Peaches. HortScience, 43, 1603-1605.
Haji, T., Yaegaki, H. and Yamaguchi, M. (2005) Inheritance and Expression of Fruit Texture Melting, Non-Melting and Stony Hard in Peach. Scientia Horticulturae, 105, 241-248. https://doi.org/10.1016/j.scienta.2005.01.017