Introgressive Hybrids of <i>Arisaema sikokianum</i> and <i>A. tosaense</i> (Araceae) Confirmed through Nuclear and Chloroplast DNA Comparisons
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Abstract
Morphologically putative introgressive hybrids of <i>Arisaema sikokianum</i> Franch. et Sav. and <i>A. tosaense</i> Makino were newly found in Kochi and Tokushima Prefectures in Japan. All the individuals have the same morphological characteristics as <i>A. tosaense</i> excluding a purple spathe. Molecular analysis using PCR-RFLP of internal transcribed spacer (ITS) in nuclear DNA (nrDNA) indicates that these putative introgressive hybrids have the same pattern as <i>A. tosaense</i>. Moreover, the sequences of chloroplast DNA (cpDNA) of the putative introgressive hybrids were identical to <i>A. sikokianum</i> from Kochi Prefecture and <i>A. tosaense</i> from Tokushima Prefecture. The results suggest that the plants are introgressive hybrids of <i>A. sikokianum</i> and <i>A. tosaense</i> and that they have highly exchanged genes with <i>A. tosaense</i>.
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