Potato late blight, caused by the oomycete <i> Phytophthora infestans </i> , is one of the most devastating diseases in the agricultural sector around the world. Many genes ( <i> R </i> genes) conferring resistance to late blight have been identified in various potato species and most of these R genes have been used in potato breeding. The aim of this study was to develop and validate PCR-based assays for the <i> R </i> genes <i> Rpi-blb1, Rpi-blb2, Rpi-blb3 </i> and <i> Rpi-bt1 </i> , to distinguish between late blight resistant and late blight susceptible potato progeny in the given breeding background. A total of 100 breeding progeny were screened for the presence of these <i> R </i> genes and tested for resistance against <i> P. infestans </i> mating type A2, genotype US-8 strain, using detached leaf and tuber rot assays. PCR products for the <i> Rpi-blb1 </i> and <i> Rpi-bt1 </i> resistance genes were identified in the resistant progeny but were absent in the susceptible ones; therefore these PCR assays could differentiate between late blight resistant and susceptible plants . Genotypic data from the DNA markers derived from the <i> Rpi-blb1 </i> and <i> Rpi-bt1 </i> genes was found to correlate with the phenotypic data for foliar late blight but not with data for tuber rot. Our results demonstrate that markers derived from these two <i> R </i> genes could be useful for marker-assisted selection (MAS) for foliar late blight resistance in potato breeding programs.
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