Developing Stable Cultivar through Microspore Mutagenesis in ×<i>Brassicoraphanus koranhort</i>, Inter-Generic Allopolyploid between <i>Brassica rapa</i> and <i>Raphanus sativus</i> — Oak Academic Publishing
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Developing Stable Cultivar through Microspore Mutagenesis in ×<i>Brassicoraphanus koranhort</i>, Inter-Generic Allopolyploid between <i>Brassica rapa</i> and <i>Raphanus sativus</i>
BioBreeding Institute, Sillyeong-Ro 153, Daeduk, Ansung, Gyeonggi, Korea
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BioBreeding Institute, Sillyeong-Ro 153, Daeduk, Ansung, Gyeonggi, Korea
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BioBreeding Institute, Sillyeong-Ro 153, Daeduk, Ansung, Gyeonggi, Korea
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Dept. of Integrative Plant Science, Chung-Ang University, Ansung, Korea
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Dept. of Integrative Plant Science, Chung-Ang University, Ansung, Korea
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Dept. of Integrative Plant Science, Chung-Ang University, Ansung, Korea
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Plant Biotechnology Institute, Department of Life Science, Sahmyook University, Seoul, Korea
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Plant Biotechnology Institute, Department of Life Science, Sahmyook University, Seoul, Korea
1 BioBreeding Institute, Sillyeong-Ro 153, Daeduk, Ansung, Gyeonggi, Korea
2 BioBreeding Institute, Sillyeong-Ro 153, Daeduk, Ansung, Gyeonggi, Korea
3 BioBreeding Institute, Sillyeong-Ro 153, Daeduk, Ansung, Gyeonggi, Korea
4 Dept. of Integrative Plant Science, Chung-Ang University, Ansung, Korea
5 Dept. of Integrative Plant Science, Chung-Ang University, Ansung, Korea
6 Dept. of Integrative Plant Science, Chung-Ang University, Ansung, Korea
7 Plant Biotechnology Institute, Department of Life Science, Sahmyook University, Seoul, Korea
8 Plant Biotechnology Institute, Department of Life Science, Sahmyook University, Seoul, Korea
A stable progeny was developed through induced mutation, using microspore culture, of the hybrid (F 1 F 1 ) produced by crossing a newly synthesized, unstable allopolyploid (F 1 ) and a stable cultivar, BB#1(F 1 ) in x Brassicoraphanus . An F 1 F 1 plant was subjected to the induced mutation system established during production of BB#1. Morphological characteristics of the progeny such as color, and leaf number and length, differed from those of BB#1. The bolting time of the progeny in spring cropping was very late compared to BB#1, allowing it to be grown to an adult plant in spring. Genomic in situ hybridization analysis of pollen mother cells at prophase I identified 19 bivalents, 10 from Brassica rapa and 9 from Raphanus sativus . The glucoraphenin content was almost identical to that of BB#1. Two cultivars are available in the baemoochae crop now. These results indicate that induced mutation using microspore culture is a viable method of stabilizing intergeneric allopolyploids between B. rapa and R. sativus .
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