Research ArticleOpen AccessGoogle Scholar indexed
Expression Vector Construction and Genetic Transformation of <i>Paeonia lactiflora</i> Gibberellin 20-Oxidase Gene
Forestry College of Shandong Agricultural University, Tai’an, China
Forestry College of Shandong Agricultural University, Tai’an, China
Forestry College of Shandong Agricultural University, Tai’an, China
Forestry College of Shandong Agricultural University, Tai’an, China
Forestry College of Shandong Agricultural University, Tai’an, China
- 1 Forestry College of Shandong Agricultural University, Tai’an, China
- 2 Forestry College of Shandong Agricultural University, Tai’an, China
- 3 Forestry College of Shandong Agricultural University, Tai’an, China
- 4 Forestry College of Shandong Agricultural University, Tai’an, China
- 5 Forestry College of Shandong Agricultural University, Tai’an, China
American Journal of Plant Sciences·Volume 08 (2017)·Pages 1525–1533·Published 13 June 2017·DOI10.4236/ajps.2017.87105
Copy link · social · email
Abstract
GA20-oxidase ( GA 20 ox ) gene encodes a key enzyme in gibberellins (GAs) biosynthesis pathway. Previously, we have cloned a PlGA 20 ox gene (GeneBank accession number: KU886552) from Paeonia lactiflora . To further reveal its function, we constructed an expression vector in the present study and then transformed it into Arabidopsis thaliana plants by floral dip method. The transgenic plants exhibited an early bolting, increased height and improved vegetative growth. These results provided efficient vector tool and functional information of PlGA 20 ox for future gene engineering in peony.
Keywords<i>PlGA</i>20<i>ox</i>Expression Vector<i>Arabidopsis thaliana</i>Transformation
- Silverstone, A.L. and Sun, T.P. (2000) Gibberelins and the Green Revolution. Trends in Plant Science, 5, 1-2. https://doi.org/10.1016/S1360-1385(99)01516-2
- Olszewski, N., Sun, T.P. and Gubler, F. (2002) Gibberellin Signaling: Biosynthesis, Catabolism, and Response Pathways. Plant Cell, 14, S61-S80.
- Xu, Y.L., Li, L., Wu, K., et al. (1995) The GA5 Locus of Arabidopsis thaliana Encodes a Multifunctional Gibberellin 20-Oxidase: Molecular Cloning and Functional Expression. Proceedings of the National Academy of Sciences, 92, 6640-6644. https://doi.org/10.1073/pnas.92.14.6640
- Desgagné-Penix, I. and Sponsel, V.M. (2008) Expression of Gibberellin 20-Oxidase1 (AtGA20ox1) in Arabidopsis Seedlings with Altered Auxin Status Is Regulated at Multiple Levels. Journal of Experimental Botany, 59, 2057-2070. https://doi.org/10.1093/jxb/ern063
- Phillips, A.L., Ward, D.A., Uknes, S., et al. (1995) Isolation and Expression of Three Gibberellin 20-oxidase cDNA Clones from Arabidopsis. Plant Physiology, 108, 1049-1057. https://doi.org/10.1104/pp.108.3.1049
- Esmeralda, M., Esther, C., Omar, R.R., et al. (2010) Hormonal Regulation of Tomato Gibberellin 20-oxidase1 Expressed in Arabidopsis. Journal of Plant Physiology, 167, 1188-1196. https://doi.org/10.1016/j.jplph.2010.03.019
- Pimenta Lange, M.J., Liebrandt, A., Arnold, L., et al. (2013) Functional Characterization of Gibberellin Oxidases from Cucumber, Cucumis sativus L. Phytochemistry, 90, 62-69. https://doi.org/10.1016/j.phytochem.2013.02.006
- Zhang, Y.X., Zhang, W.C., Li, Y.E., et al. (2014) The Study of PsGA20ox Gene Participating in Endo-Dormancy Release of Flower Buds by Chilling Treatment in Tree Peony. Acta Botanica Boreali-Occidentalia Sinica, 29, 22-26.
- Eriksson, M.E., Israelsson, M., Olsson, O., et al. (2000) Increased Gibberellin Biosynthesis in Transgenic Trees Promotes Growth, Biomass Production and Xylem Fiber Length. Nature Biotechnology, 18, 784-788. https://doi.org/10.1038/77355
- Olimpieri, I., Caccia, R., Picarella, M.E., et al. (2011) Constitutive Cosuppression of the GA20-oxidase1 Gene in Tomato Leads to Severe Defects in Vegetative and Reproductive Development. Plant Science, 180, 496-503. https://doi.org/10.1016/j.plantsci.2010.11.004
- Deng, W., Li, T., Luo, K.M., et al. (2008) Transformation of Gibberellin 20-Oxidase Gene of Cotton into Chinese White Poplar. Acta Botanica Boreali-Occidentalia Sinica, 28, 1095-1100.