The present study is about <i> in vitro </i> culture of cork oak, through callogenesis from nodes and internodes, withdrawn from seedlings of three months. These latter were obtained after acorns germination on peat. Nodes showed a high capacity for callogenesis and the best rate was obtained on a medium containing Woody Plant Medium (WPM) macronutrients, Murashige and Skoog (MS) micronutrients and vitamins, 4.5 μM of 6-Benzylaminopurine (BAP), 7 μM of 2-naphthalineacetic acid (NAA) and 30 g/L of Sucrose. Calluses are transplanted onto the same mineral solution, with or without growth regulators, and in the 3rd transplanting; small white embryos appear on the surface of calluses.
Ajbilou, R., Maranón, T. and Arroyo, J. (2006) Ecological and Biogeographical Analyses of Mediterranean Forests of Northern Morocco. Acta Oecologica, 29, 104-113. https://doi.org/10.1016/j.actao.2005.08.006
Benzyane, M. (1997) Improvement Strategy of Forest Tree Species in Morocco: The Case of Cork Oak. Quercus Suber Network. Report of the Third and Fourth Meetings, 9-12 June 1996 (Italy), 20-22 February 1997 (Spain), 60-67.
Benzyane, M. (1998) The Moroccan Forest, Economic and Social Product to Be Developed. In: Proceedings of the Mediterranean Seminar on the Vegetation of Cork Oak Forests. Tabarka, 22-24 October 1996, Annals of INGREF, Special Issue, 12-21.
Boudy, P. (1950) North African Forest Economy. Monograph and Treatment of Tree Species. Larose, Paris, 525.
Bajaj, Y.P.S. (1986) Biotechnology of Tree Improvement for Rapid Propagation and Biomass Energy Production. In: Biotechnology in Agriculture and Forestry, Chapter Trees I, Vol 1, Springer-Verlag, Berlin, 1-23. https://doi.org/10.1007/978-3-642-70576-2_1
Vasil, I. and Hildebrandt, A. (1966) Variations of Morphogenetic Behaviour in Plants Tissue Cultures. II. Petroselinum hortense. American Journal of Botany, 53, 869-874. https://doi.org/10.2307/2439808
Banerjee, S. and Gupta, S. (1975) Morphogenesis in Tissue Cultures of Different Organes of Nigella sativa. Physiologia Plantarum, 33, 185-187. https://doi.org/10.1111/j.1399-3054.1975.tb03790.x
Hunault, G. (1979) Research on Behavior of Organ and Tissue Fragments of In Vitro Cultivated Monocotyledons. III. Study of the Case of Several Liliaceae. Revue de cytologie et de biologie végétales, 2, 103-154.
Novak, F.J., Opatrny, Z., Rovenska, B. and Nesticky, M. (1979) Studies on the Morphogenetic Response of Maize Tissue Cultures of Different Origin. Biologia Plantarum, 21, 418-426. https://doi.org/10.1007/BF02889482
Jacquiot, C. (1952) On the Phenomena of Histogenesis Observed in In Vitro Cultures of Cambia Tissue of Oaks (Quercus sessiliflora Sm., Quercus pedunculata Ehr., Q. suber L.). Comptes rendus hebdomadaires des séances de l’Académie des sciences, 234, 1468-1470.
El Maataoui, M. and Espagnac, H. (1987) Neoformation of Somatic Embryonic Type Structures on Cork Oak Tissue Cultures (Quercus suber L.). Comptes Rendus de l’Académie des Sciences. Série 3, 304, 83-88.
Feraud-Keller, C., El Maataoui, M., Gouin, O. and Espagnac, H. (1989) Somatic Embryogenesis in three Species of Mediterranean Oaks. Annals of Forest Science, 46, 130-132.
El Maataoui, M., Espagnac, H. and Michaux-Ferriere, N. (1990) Histology of Callogenesis and Somatic Embryogenesis Induced in Stem Fragments of Cork Oak (Quercus suber) Cultured In Vitro. Annals of Botany, 66, 183-190. https://doi.org/10.1093/oxfordjournals.aob.a088014
McCown, B.H. and Lloyd, G. (1981) Woody Plant Medium (WPM)—A Mineral Nutrient Formulation for Microculture of Woody Plant Species. HortScience, 16, 453-453.
Murashige, T. and Skoog, F. (1962) A Revised Medium for Rapid Growth and Bioassays with Tobacco Tissue Culture. Physiologia Plantarum, 15, 473-497. https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
Schenk, R.V. and Hildebrandt, A.C. (1972) Medium and Techniques for Induction and Growth of Monocotyledonous and Dicotyledonous Plant Cell Cultures. Canadian Journal of Botany, 50, 199-204. https://doi.org/10.1139/b72-026
Klein, R.M. (1963) Interaction of Ultraviolet and Visible Radiation on the Growth of Cell Aggregates of Ginkgo Pollen Tissue. Physiologia Plantarum, 16, 73-81. https://doi.org/10.1111/j.1399-3054.1963.tb08291.x
Das, N.K., Patau, K. and Skoog, F. (1956) Initiation of Mitosis and Cell Division by Kinetin and Indolacetic Acid in Excised Tobacco Pith Tissue. Physiologia Plantarum, 9, 640-651. https://doi.org/10.1111/j.1399-3054.1956.tb07826.x
Skoog, F. and Miller, C.O. (1957) Chemical Regulation of Growth and Organ Formation in Plant Tissues Cultured In Vitro. Symposia of the Society for Experimental Biology, 11, 118-131.
Letham, D.S. (1969) Cytokinins and Their Relation to Other Phytohormones. Bio-Science, 19, 309-316. https://doi.org/10.2307/1294513
Heller, R. (1978) Abstract of Vegetal Physiology. Volume II: Development. Masson, Paris.
Flick, C.E., Evans, D.A. and Sharp, W.R. (1983) Organogenesis. In: Sharp, W.R., Evans, D.A., Ammirato, P.V. and Yamada, Y., Eds., Handbook of Plant Cell Culture, Vol. 1, Macmillan Publishing Company, New York, 13-81.
Aboel-Nil, M.M. (1987) Tissue Culture of Douglas-Fir and Western North American Conifers. In: Bonga, J.M. and Durzan, D.J., Eds., Cell and Tissue Culture in Forestry, Vol. 3, Martinus Nijhoff Publishers, Dordrecht, 80-100. https://doi.org/10.1007/978-94-017-0992-7_6
Jacquiot, C. (1956) On the Requirements in Auxines and the External Morphological Characters of the Cultures of Cambial Tissue of some Tree Species. Comptes Rendus Hebdomadaires des Séances de l’Académie des Sciences, 243, 510-512.
Feraud, C. (1985) Some Aspects of Green Oak Growth and Development (Quercus ilex L.) and First In Vitro Culture Tests. Thesis of 3rd Cycle, Faculty of Sciences and Techniques of Saint-Jérome, Aix-Marseille III.
Gamborg, O.L. (1966) Aromatic Metabolism in Plants. II. Enzymes of the Shikimate Pathway in Suspension Cultures of Plant Cells. Canadian Journal of Biochemistry, 44, 791-799. https://doi.org/10.1139/o66-097
Rancillac, M., El-Hamri, I. and Klinguer, A. (1994) Expression of Somatic Embryogenesis from Leaf Discs in a Forest Tree, the American Red Oak. Plant Science, 78.
Pinto, G., Valentim, H., Costa, A., Castro, S. and Santos, C. (2002) Somatic Embryogenesis in Leaf Callus from a Mature Quercus suber L. Tree. In Vitro Cellular and Developmental Biology Plant, 38, 569-572. https://doi.org/10.1079/IVP2002352
Loureiro, J., Pinto, G., Lopes, T., Dolezel, J. and Santos, C. (2005) Assessment of Ploidy Stability of the Somatic Embryogenesis Process in Quercus suber L. Using Flow Cytometry. Planta, 221, 815-822. https://doi.org/10.1007/s00425-005-1492-x
Nishikawa, K., Tanaka, N., Nakanishi, F., Shimomura, K. and Ishimaru, K. (1997) Tannins and Related Phenolics in Callus and Root Cultures of Quercus glauca. Plant Biotechnology, 14, 123-125. https://doi.org/10.5511/plantbiotechnology.14.123
Krajci, I. and Gross, G.G. (1987) Formation of Gallotannins in Callus Cultures from Oak (Quercus robur). Phytochemistry, 26, 141-143.
Linsmaier, E.M. and Skoog, F. (1965) Organic Growth Factor Requirements of Tobacco Tissue Cultures. Physiologia Plantarum, 18, 100-127. https://doi.org/10.1111/j.1399-3054.1965.tb06874.x
Pevalek-Kozlina, B. and Jelaska, S. (1995) The Regeneration Ability in Common Oak (Quercus robur L.) Callus Cultures. Acta Pharmaceutica, 45, 165-398.
El Kbiach, M.L., Lamarti, A. and Badoc, A. (2001) In Vitro Culture of Axillary Buds of Cork Oak (Quercus suber L.). I. Influence of Cytokinins on the Organogenesis and Callogenesis of Seed Nodes. Bulletin de la Société de Pharmacie de Bordeaux, 140, 67-82.
Seckinger, G.R., McCown, B.H. and Struckmeyer, B.E. (1979) Production of Anomalous Structures in Quercus rubra L. Callus Cultures. American Journal of Botany, 66, 993-996. https://doi.org/10.2307/2442244
Seckinger, G.R., McCown, B.H., Struckmeyer, B.E. and Durbin, R.D. (1978) Production and Rapid Multiplication of Organoids from Callus Cultures of Quercus rubra L. (Red Oak). HortScience, 13, 355-355.
Cuenca, B., San-Jose, M.C., Martinez, M.T., Ballester, A. and Vieitez, A.M. (1999) Somatic Embryogenesis from Stem and Leaf Explants of Quercus robur L. Plant Cell Reports, 18, 538-543. https://doi.org/10.1007/s002990050618