Adventitious budding from embryonic cotyledons of immature seeds of carob was obtained. The combination of BAP (4.44 μM) and NAA (1.5 μM) furthered the neoformation of adventitious buds. These latter were multiplied on MS medium added with BAP (2.22 μM). Stems and leaves growing were improved by adding 2.02 μM GA 3 . Elongation was favored by 0.5 μM NAA. 70% of rooting was obtained with 10 μM IBA.
Gullo, M.A. and Salleo, S. (1988) Different Strategies of Drought Resistance in Three Mediterranean Sclerophyllous Trees Growing in the Same Environmental Conditions. New Phytologist, 108, 267-276. https://doi.org/10.1111/j.1469-8137.1988.tb04162.x
Correia, P.M. and Martins-Loução, M.A. (1994) Preliminary Studies on Mycorrrhizae of Ceratonia siliqua L., Mycorrhizas in Integrated Systems from Genes to Plant Development. New York Botanical Gardens Bronx, NY, 86-88.
Russo, G. and Polignano, G.B. (1996) Variation of Seed and Fruit Characters in Ceratonia siliqua L. Cultivars. Genetic Resources and Crop Evolution, 43, 525-531. https://doi.org/10.1007/BF00138829
Batlle, I. and Tous, J. (1997) Carob Tree. Ceratonia siliqua L. International Plant Genetic Resources Institute, Rome, 92 p.
Lee, C.L., Paul, J.L. and Hackett, W.P. (1977) Promoting of Rooting in Stem Cuttings of Several Ornamental Plants by Pretreatment with Acid or Base. HortScience, 12, 41-42.
Romano, A., Barros, S. and Martins-Loução, M.A. (2002) Micropropagation of the Mediterranean Tree Ceratonia siliqua L. Plant Cell, Tissue and Organ Culture, 68, 35-41. https://doi.org/10.1023/A:1012912504288
Custódio, L., Martins-Loução, M.A. and Romano, A. (2004) Influence of Sugars on in vitro Rooting and Acclimatization of Carob Tree. Biologia Plantarum, 48, 469-472. https://doi.org/10.1023/B:BIOP.0000041107.23191.8c
Gonçalves, S., Correia, P.J., Martins-Loução, M.A. and Romano, A. (2005) A New Medium Formulation for in vitro Rooting of Carob Tree based on Leaf Macronutients Concentrations. Biologia Plantarum, 49, 277-280. https://doi.org/10.1007/s10535-005-7280-4
Shahzad, A., Akhtar, R., Bukhari, N.A. and Perveen, K. (2017) High Incidence Regeneration System in Ceratonia siliqua L. Articulated with SEM and Biochemical Analysis during Developmental Stages. Trees, 31, 1149-1163. https://doi.org/10.1007/s00468-017-1534-6
Canhoto, J.M., Rama, S.C. and Cruz, G.S. (2006) Somatic Embryogenesis and Plant Regeneration in Carob (Ceratonia siliqua L.). In Vitro Cellular & Developmental Biology-Plant, 42, 514-519. https://doi.org/10.1079/IVP2006819
Lozzi, A., Abousalim, A. and Abdelwahd, R. (2015) Effet du 2,4-D sur l’Induction de l’Embryogenèse Somatique à partir de Cotylédons Matures de Caroubier (Ceratonia siliqua L.). Revue Marocaine des sciences Agronomiques et Vétérinaires, 3, 24-29.
Custódio, L. and Romano, A. (2006) In vitro Morphogenesis in Zygotic Embryo Cultures of Carob Tree (Ceratonia siliqua L.). Acta Horticulturae, 725, 477-481. https://doi.org/10.17660/ActaHortic.2006.725.68
Ksia, E., Harzallah-Skhiri, F., Verdeil, J.L., Gouta, H., Alemanno, L. and Bouzid, S. (2008) Somatic Embryo Production from Immature Seeds of Carob (Ceratonia siliqua L.): Histological Evidence. The Journal of Horticultural Science and Biotechnology, 83, 401-406. https://doi.org/10.1080/14620316.2008.11512398
McCown, B.H. and Lloyd, G. (1981) Woody Plant Medium (WPM)—A Mineral Nutrient Formulation for Microculture of Woody Plant Species. HortScience, 16, 453-453.
Murashige, T. and Skoog, F. (1962) A Revised Medium for Rapid Growth and Bioassays with Tobacco Tissue Culture. Physiologia Plantarum, 15, 473-497. https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
Radojevic, I., Marinkovic, N. and Jevremovic, S. (2000) Influence of the Sex of Flowers on Androgenesis in Aesculus hippocastanum L. Anther Culture. In vitro Cellular & Developmental Biology-Plant, 36, 464-469. https://doi.org/10.1007/s11627-000-0083-6
Chaturvedi, R., Razdan, M.K. and Bhojwani, S.S. (2003) Production of Haploids of Neem (Azadirachta indica A. Juss.) by Anther Culture. Plant Cell Reports, 21, 531-537.
Custódio, L., Carneiro, M.F. and Romano, A. (2004) Microsporogenesis and Anther Culture in Carob Tree (Ceratonia siliqua L.). Scientia Horticulturae, 104, 65-77. https://doi.org/10.1016/j.scienta.2004.08.001
Carimi, F., Di Lorenzo, R. and Crescimanno, F.G. (1997) Callus Induction and Somatic Embryogenesis in Carob (Ceratonia siliqua L.) from Ovule Culture. Scientia Horticulturae, 70, 73-79. https://doi.org/10.1016/S0304-4238(97)00034-4
Belaizi, M., Bolen, M.R. and Boxus, P. (1995) Régénération in vitro et Acclimatation du Caroubier (Ceratonia siliqua L.). In: Dubois, J., Ed., Quel Avenir pour l’Amélioration des Plantes? John Libbey Eurotext, Paris, 227-232.
Naghmouchi, S., Khoudja, M.L., Agusti Romero, A. and Boussaid, M. (2012) Micropropagation of Carob, Ceratonia siliqua L., by Apex Culture. Acta Botanica Gallica, 159, 357-361. https://doi.org/10.1080/12538078.2012.737124
Radi, A., Echchgadda, G., Ibijbijen, J. and Rochd, M. (2013) In vitro Propagation of Moroccan Carob (Ceratonia siliqua L.). Journal of Food, Agriculture & Environment, 11, 1103-1107.
Gharnit, N. and Ennabili, A. (2009) Essais Préliminaires de Culture in vitro du Caroubier (Ceratonia siliqua L.) Originaire du Nord Ouest du Maroc. Biomatec Echo, 3, 18-25.
Gresshoff, P.M. and Doy, C.H. (1972) Development and Differentiation of Haploid Lycopersicum esculentum (Tomato). Planta, 107, 161-170. https://doi.org/10.1007/BF00387721
Sebastian, K.T. and McComb, J.A. (1986) A Micropropagation System for Carob (Ceratonia siliqua L.). Scientia Horticulturae, 28, 127-131. https://doi.org/10.1016/0304-4238(86)90132-9
Hakim, L., Islam, M.R., Mamun, A.N.K., Ahmed, G. and Khan, R. (2010) Clonal Propagation of Carob (Ceratonia siliqua L., Fabaceae). Bangladesh Journal of Botany, 39, 15-19. https://doi.org/10.3329/bjb.v39i1.5520
Saidi, R., Lamarti, A. and Badoc, A. (2007) Micropropagation du Caroubier (Ceratonia siliqua) par Culture de Bourgeons Axillaires issus de Jeunes Plantules. Bulletin de la Société de Pharmacie de Bordeaux, 146, 113-129.
Thomas, V. and Mehta, A.R. (1983) Effect of Phloroglucinol on Shoot Growth and Initiation of Roots in Carob Tree Cultures Grown in Vitro. In Sen, S.K. and Giles, K.L., Eds., Plant Cell Culture in Crop Improvement. Plenum Press, New York, 451-457. https://doi.org/10.1007/978-1-4684-4379-0_49
Saidi, R., El Bouzdoudi, B., Kbiach, M.B., Lamarti, A. and Maouni, A. (2015) Effets des Macroéléments et des Auxines sur la Micropropagation du Caroubier (Ceratonia siliqua L., Leguminosae) par Culture d’Apex. Journal of Materials and Environmental Sciences, 6, 2330-2337.
Victório, C.P., Salgueiro Lage, C.L. and Sato, A. (2012) Tissue Culture Techniques in the Proliferation of Shoots and Roots of Calendula officinalis. Revista Ciência Agronômica, 43, 539-545. https://doi.org/10.1590/S1806-66902012000300017
Sadeghi, F., Yadollahi, A., Jafarkhani Kermani, M. and Eftekhari, M. (2015) Optimizing Culture Media for in vitro Proliferation and Rooting of Tetra (Prunus empyrean 3) Rootstock. Journal of Genetic Engineering and Biotechnology, 13, 19-23. https://doi.org/10.1016/j.jgeb.2014.12.006
Belaizi, M., Sangwan, R.S., David, A. and Sangwan-Norreel, B.S. (1989) Maitrise des étapes de la micropropagation du Pommier (Pyrus malus) L. cv. Golden Delicious, Bulletin de la Société Botanique de France. Lettres Botaniques, 136, 187-197.
Riffaud, J.L., Cornu, D. and Capelli, P. (1981) Utilisation de la Culture in vitro Pour la Multiplication de Merisiers Adultes (Prunus avium L.) Sélectionnés en Forêt. Agronomie, EDP Sciences, 1, 633-640. https://doi.org/10.1051/agro:19810804
Boxus, P. and Quoirin, M. (1974) La Culture de Méristèmes Apicaux de quelques Espèces de Prunus. Bulletin de la Société Royale de Botanique de Belgique, 107, 91-101.
Saidi, R., Wahby, I. and Lamarti, A. (2003) Multiplication in vitro du Caroubier (Ceratonia siliqua L.) par Culture des Bourgeons. Proceedings of 3ème Congrès National de Génétique et Biologie Moléculaire, Tanger Maroc, 98 p.
Onay, A., Pirinc, V., Adiyaman, F., Isikalan, ç., Tilkat, E. and Basaran, D. (2003) In Vivo and in vitro Micrografting of Pistachio, Pistacia vera L. cv. Turkish Journal of Biology, 27, 95-100.
Bhatt, I.D. and Dhar, U. (2004) Factors Controlling Micropropagation of Myrica esculenta buch. -Ham. ex D. Don: a High Value Wild Edible of Kumaun Himalaya. African Journal of Biotechnology, 3, 534-540. https://doi.org/10.5897/AJB2004.000-2097
Shwab, L. and Martins-Loução, M.A. (1988) Shoot Formation in Ceratonia siliqua Hypocotyls Callus. Proceedings of the 2nd International Carob Symposium, Valencia, 29 September-1 October 1987, 245-253.
El Hamdouni, E.M., Lamarti, A. and Badoc, A. (2000) Micropropagation des Cultivars ‘Chandler’ et ‘Tudla’ de Fraisier (Fragaria X ananassa Duch.). Bulletin de la Société de Pharmacie de Bordeaux, 139, 91-104.
Chalupa, V. (2002) In vitro Propagation of Mature Trees of Sorbus aucuparia L. and Field Performance of Micropropagated Trees. Prague Czech Academy of Agricultural Sciences, 48, 529-535.
Naghmouchi, S., Khouja, M.L., Rejeb, M.N. and Boussaid, M. (2008) Effect of Growth Regulators and Explant Origin on in vitro Propagation of Ceratonia siliqua L. via Cuttings. Biotechnologie, Agronomie, Société et Environnement, 12, 251-258.
Vinterhalter, D. and Vinterhalter, B. (1992) Factors Affecting in vitro Propagation of Carob (Ceratonia siliqua L.). Archives of Biological Sciences, 44, 177-186.