Evaluation of Substrates for Mass Culture of <i>Phaeoacremonium parasiticum</i>, the Fungus Responsible for Artificial Oleoresin Deposition in Agarwood Plant — Oak Academic Publishing
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Evaluation of Substrates for Mass Culture of <i>Phaeoacremonium parasiticum</i>, the Fungus Responsible for Artificial Oleoresin Deposition in Agarwood Plant
Department of Plant Pathology, Assam Agricultural University, Jorhat, India
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Department of Plant Pathology, Assam Agricultural University, Jorhat, India
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Department of Plant Pathology, Assam Agricultural University, Jorhat, India
1 Department of Plant Pathology, Assam Agricultural University, Jorhat, India
2 Department of Plant Pathology, Assam Agricultural University, Jorhat, India
3 Department of Plant Pathology, Assam Agricultural University, Jorhat, India
For establishing the favourable media for growth of <i> Phaeoacremonium parasiticum</i>, the fungus was inoculated in five different media (Potato Dextrose Broth (PDB), Host Extract (50%) + PDB (50%) (HE + PDB), and the solid media viz. , Rice Bran (RB), Maize Meal (MM) and Wheat Bran (WB)) at pH of 6.5 and incubated at a temperature of 30℃ ± 1℃. The solid media were added with water at the rate of 70 ml/100g of solid substrate. After 30 days of inoculation, conidial population of <i> P. parasiticum</i> in liquid media was lower compared to conidial population in solid media . In solid media, MM media showed the highest conidial population (9.56 log·cfu/g) followed by WB (9.50 log·cfu/g) and RB (9.38 log·cfu/g). Hence, Maize Meal media (MM: Water = 100 g:70 ml) at pH of 6.5, incubation temperature: 30℃ ± 1℃for 30 days would be the standard technique for mass production of <i> P. parasiticum </i> .
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