Development of a Procedure to Maximize Production of Hardy Rootstocks of Citrus Using Stem Cuttings
- 1 University of Florida Institute of Food and Agricultural Science Mid-Florida Research and Education Center, Apopka, USA
- 2 Agromillora Florida, Inc., Wildwood, USA
Abstract
Shoots of Citrus sp . Kuharske were used to develop protocols for rooting reportedly HLB resistance rootstocks under intermittent mist. Investigated were shoot maturity, nodes per cutting, leaves per cutting, effects of buds, auxin concentrations and auxin solvent. Shoot maturity was most influential for success, with cuttings taken below the first 30 cm of active terminal growth producing greater root generation. Use of a thickening agent (Natrosal) to dilute the commercial auxin was second most in importance for rooting success. Root mass increased with increasing number of leaves. Cutting stems between nodes or below the lowest bud were inconsequential. To produce maximum number of viable cuttings, single node-single leaf cuttings were preferred. Single bud cuttings produced one shoot after rooting. This was adventitious since multi-node cuttings usually sprouted new shoots that would need to be removed before budded. Evaluation of the best combination of auxin and cutting-related attributes were evaluated with four additional common rootstocks in June 2016. Rooting was 100% successful. A quick dip (0.5 s) in a 7500 ppm solution of Dip&Gro produced the most root generation in six weeks for all rootstocks. Root quantity varied by rootstock.
- Johnson, E.G., Wu, J., Bright, D.B. and Graham, J.H.(2014) Association of “Candidatus Liberibacter Asiaticus” Root Infection, but not Phloem Plugging with Root Loss on Huaglongbing-Affected Trees Prior to Appearance of Foliar Symptoms. Plant Pathology, 63, 290-298. https://doi.org/10.1111/ppa.12109
- Grosser, J.W. (2013) Personal Communication, 20 June 2013. Verbal Communication of Non-Published Results.
- Conger, O.H. (1889) Lemon Culture. Annual Report of the Secretary of the State Board of Michigan, 361-365.
- Cooper, W.C. (1940) Rooting Citrus Cuttings with Synthetic Growth-Substances. Proceedings of the Florida State Horticulture Society, 53, 174-177.
- Ochse, J.J. and Reark, J.B. (1950) The Propagation of Sub-Tropical Fruit Plants by Cuttings, A Progress Report. Proceedings of the Florida State Horticulture Society, 63, 248-251.
- Rieger, M. (1992) Growth, Gas Exchange, Water Uptake, and Drought Response of Seedling-and-Cutting Propagated Peach and Citrus Rootstocks. Journal of the American Society for Horticultural Science, 117, 834-840.
- Sabbah, S.M., Grosser, J.W., Chandler, J.L. and Louzada, E.S. (1991) The Effect of Growth Regulators on the Rooting of Stem Cuttings of Citrus, Related Genera and Intergeneric Somatic Hybrids. Proceedings of the Florida State Horticulture Society, 104, 188-191.
- Ferguson, J., Young, M. and Halvorson, J. (1985) The Propagation of Citrus Rootstocks by Stem Cuttings. Proceedings of the Florida State Horticulture Society, 98, 39-42.
- Seran, T.H. and Umadevi, T. (2011) Influence of Indole Acetic Acid (IAA) on the Establishment of Stem Cuttings in Lemon (Citrus limon L.). Journal of Agricultural Research, 49, 517-524.
- Beeson Jr., R.C. (2000) Putting the Speed Back in Quick-dip Auxin Application. SNA Research Conference, 45, 298-302.
- Foster, G.S., Campbell, R.K. and Adams, W.T. (1984) Heritability, Gain, and C Effects in Rooting of Western Hemlock Cuttings. Canadian Journal of Forest Research, 14, 628-638. https://doi.org/10.1139/x84-114
- Macht, D.I. and Grumbein, M.L. (1937) Influence of Indole Acetic, Indole Butyric, and Naphthalene Acetic Acids on Roots of Lupinus Albus Seedlings. American Journal of Botany, 24, 457-460. https://doi.org/10.2307/2436433