Rooting of Stem Cuttings with Different Indole 3 Butyric Acid (IBA) Treatments and Development of Micropropagation Protocol for <i>Piper betle</i> L. Node Culture — Oak Academic Publishing
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Rooting of Stem Cuttings with Different Indole 3 Butyric Acid (IBA) Treatments and Development of Micropropagation Protocol for <i>Piper betle</i> L. Node Culture
Technical Institute of Babel, Al-Furat Al-Awsat Technical University, Al Hillah, Iraq
,
Laboratory of Plant Microstructure and Anatomy, Department of Crop Science, Faculty of Agriculture, Universiti Putra Malaysia, Serdang, Malaysia
,
In Vitro Laboratory Department of Agriculture Technology, Universiti Putra Malaysia, Serdang, Malaysia
,
Department of Crop Science, Faculty of Agriculture, Universiti Putra Malaysia, Serdang, Malaysia
1 Technical Institute of Babel, Al-Furat Al-Awsat Technical University, Al Hillah, Iraq
2 Laboratory of Plant Microstructure and Anatomy, Department of Crop Science, Faculty of Agriculture, Universiti Putra Malaysia, Serdang, Malaysia
3 In Vitro Laboratory Department of Agriculture Technology, Universiti Putra Malaysia, Serdang, Malaysia
4 Department of Crop Science, Faculty of Agriculture, Universiti Putra Malaysia, Serdang, Malaysia
The present study, conducted during 2016 and 2017 seasons, aimed to investigate the effect of IBA on rooting of Piper betle L. stem cuttings (softwood and semi-hardwood). The experiment was undertaken in misting house field 2 UPM using the sand media to determine the adventitious roots initiation and development using the histological method. The cuttings were treated with different IBA concentrations (0, 500, 1000, 1500 and 2000 mg/L). The nodes explants were used in the development of a protocol for in vitro propagation of P. betle L., with different concentrations of Clorox with different times of immersion (20% Clorox 10 minutes, 30% Clorox 10 minutes, 20% Clorox 20 minutes, and 30% 20 minutes). In multiplication of the plantlets, Murashige and Skoog (MS) medium with different concentrations of BAP (0, 0.5, 1.0, 2.0 mg/L) were used to investigate the rooting of the explants. The results indicated that the type s of the cuttings were different in the rooting capacity and the length of the roots. Moreover, it was found that in comparison with the control treatment, by a rise in the concentrations of the IBA, there was a significant upsurge in the rooting percentage, the root diameter, and the number of the roots. The results indicated that the types of cutting with 1000, 1500 and 2000 mg/L IBA perform better in the root percentage (100%) in the semi hardwood cuttings. The best results, however, were 2000 mg/L IBA in the semi hardwood cuttings, with the number of the roots to be 35.05, and the fresh weight of the roots to be 3.94 g, the dry weight of the roots to be 0.33 g, the length of the roots to be 391.88 cm, the roots diameter to be 1.21 mm, the surface area of the roots to be 121.83 cm 2 , and the root volume to be 2.99 cm 3 . Nonetheless, the optimal concentration of Clorox with the time immersion was 20% with the 20-minute immersion time, which produced a shoot induction percentage of 30% dead explants and a mean number of 70.00 shoots per explant and the optimal concentration of benzylaminopurine (BAP) at 1.0 mg/L. It is of note that a shoot induction percentage of 22.29% and a mean number of 4.1% number of auxiliary bud per treatment. P. betle shoots in MS medium without PGR MS (0.0) yielded a good rooting.
Datta, A., Ghoshdastidar, S. and Singh, M. (2011) Antimicrobial Property of Piper betel Leaf against Clinical Isolates of Bacteria. International Journal of Pharma Sciences and Research, 2, 104-109.
Sulaiman, S.F., et al. (2011) Effect of Solvents in Extracting Polyphenols and Antioxidants of Selected Raw Vegetables. Journal of Food Composition and Analysis, 24, 506-515. https://doi.org/10.1016/j.jfca.2011.01.020
Tan, Y.P. and Chan, E.W.C. (2014) Antioxidant, Antityrosinase and Antibacterial Properties of Fresh and Processed Leaves of Anacardium occidentale and Piper betle. Food Bioscience, 6, 17-23. https://doi.org/10.1016/j.fbio.2014.03.001
Pradhan, D., et al. (2013) Golden Heart of the Nature: Piper betle L. Journal of Pharmacognosy and Phytochemistry, 1, No. 6.
Babu, K.N., et al. (1992) Micropropagation of Betel Vine (Piper betle L.). Journal of Spices and Aromatic Crops, 1, 160-162.
Kumar, N., et al. (2010) Piper betle Linn. A Maligned Pan-Asiatic Plant with an Array of Pharmacological Activities and Prospects for Drug Discovery. Current Science, 99, 922-932.
McKey, D., et al. (2010) The Evolutionary Ecology of Clonally Propagated Domesticated Plants. New Phytologist, 186, 318-332. https://doi.org/10.1111/j.1469-8137.2010.03210.x
De Klerk, G.-J. (2002) Rooting of Microcuttings: Theory and Practice. In Vitro Cellular & Developmental Biology-Plant, 38, 415-422. https://doi.org/10.1079/IVP2002335
Steffens, B., Wang, J. and Sauter, M. (2006) Interactions between Ethylene, Gibberellin and Abscisic acid Regulate Emergence and Growth Rate of Adventitious Roots in Deepwater Rice. Planta, 223, 604-612. https://doi.org/10.1007/s00425-005-0111-1
Kevers, C., et al. (1997) Hormonal Control of Adventitious Rooting: Progress and Questions. Journal of Applied Botany, 71, 71-79.
Haissig, B.E. and Davis, T.D. (1994) A Historical Evaluation of Adventitious Rooting Research to 1993, in Biology of Adventitious Root Formation. Springer, 275-331. https://doi.org/10.1007/978-1-4757-9492-2_19
Han, H., Zhang, S. and Sun, X. (2009) A Review on the Molecular Mechanism of Plants Rooting Modulated by Auxin. African Journal of Biotechnology, 8.
Heloir, M.-C., et al. (1996) Changes in the Concentrations of Auxins and Polyamines during Rooting of In-Vitro-Propagated Walnut Shoots. Tree Physiology, 16, 515-519. https://doi.org/10.1093/treephys/16.5.515
Indole-3-Butyric Acid
Micropropagation
Davies, P.J. (2010) The Plant Hormones: Their Nature, Occurrence, and Functions, in Plant Hormones. Springer, 1-15. https://doi.org/10.1007/978-1-4020-2686-7_1
Tripathi, L. and Tripathi, J.N. (2003) Role of Biotechnology in Medicinal Plants. Tropical Journal of Pharmaceutical Research, 2, 243-253.
Vieitez, A., et al. (2009) In Vitro Regeneration of the Important North American Oak Species Quercus alba, Quercus bicolor and Quercus rubra. Plant Cell, Tissue and Organ Culture (PCTOC), 98, 135-145. https://doi.org/10.1007/s11240-009-9546-6
Ahmad, N., et al. (2010) Efficient Regeneration and Antioxidant Potential in Regenerated Tissues of Piper nigrum L. Plant Cell, Tissue and Organ Culture (PCTOC), 102, 129-134. https://doi.org/10.1007/s11240-010-9712-x
Wu, J.-H., et al. (2009) Factors Affecting the Efficiency of Micropropagation from Lateral Buds and Shoot Tips of Rubus. Plant Cell, Tissue and Organ Culture (PCTOC), 99, 17-25. https://doi.org/10.1007/s11240-009-9571-5
Hartmann, H.T., Kester, D.E., Davies, F.T. and Geneve, R.L. (2002) Plant Propagation: Principles and Practices. Prentice-Hall.
Kalyoncu, I., et al. (2009) Effects of Humidity Level and IBA Dose Application on the Softwood Top Cuttings of White Mulberry (Morus alba L.) and Black Mulberry (Morus nigra L.) Types. African Journal of Biotechnology, 8.
Johansen, D.A. (1940) Plant Microtechnique. McGraw-Hill Book Company, Inc., London, 530 p.
Jensen, W.A. (1962) Botanical Histochemistry: Principles and Practice.
Naija, S., et al. (2008) Anatomical and Biochemical Changes during Adventitious Rooting of Apple Rootstocks MM 106 Cultured in Vitro. Comptes rendus biologies, 331, 518-525.
O’Brien, T.P. and McCully, M.E. (1981) The Study of Plant Structure: Principles and Selected Methods. Termarcarphi Pty. Ltd., Melbourne.
Murashige, T. and Skoog, F. (1962) A Revised Medium for Rapid Growth and Bio Assays with Tobacco Tissue Cultures. Physiologia plantarum, 15, 473-497. https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
Siddiqui, M.I. and Hussain, S.A. (2007) Effect of Indole Butyric Acid and Types of Cuttings on Root Initiation of Ficus hawaii. Sarhad Journal of Agriculture, 23, 919.
Kochhar, S., et al. (2005) Differential Rooting and Sprouting Behaviour of Two Jatropha Species and Associated Physiological and Biochemical Changes. Current Science, 89, 936-939.
Basak, U., Das, A. and Das, P. (2000) Rooting Response in Stem Cuttings from Five Species of Mangrove Trees: Effect of Auxins and Enzyme Activities. Marine Biology, 136, 185-189. https://doi.org/10.1007/s002270050021
LeBude, A.V. (2005) Adventitious Rooting and Physiology of Stem Cuttings of Loblolly Pine.
Harbage, J.F., Stimart, D.P. and Evert, R.F. (1993) Anatomy of Adventitious Root Formation in Microcuttings of Malus domestica Borkh. Gala’. Journal of the American Society for Horticultural Science, 118, 680-688.
Davies Jr, F., Lazarte, J. and Joiner, J. (1982) Initiation and Development of Roots in Juvenile and Mature Leaf Bud Cuttings of Ficus pumila L. American Journal of Botany, 804-811. https://doi.org/10.2307/2442971
Girouard, R.M. (1967) Initiation and Development of Adventitious Roots in Stem Cuttings of Hedera helix: Anatomical Studies of the Juvenile Growth Phase. Canadian Journal of Botany, 45, 1877-1881. https://doi.org/10.1139/b67-202
Lovell, P.H. and White, J. (1986) Anatomical Changes during Adventitious Root Formation. New Root Formation in Plants and Cuttings. Martinus Nijhoff Publishers, Dordrecht, 111-140.
Davies, F.T. (1978) A Histological and Physiological Analysis of Adventitious Root Formation in Juvenile and Mature Cuttings of Ficus pumila L. University of Florida.
Puri, S. and Verma, R. (1996) Vegetative Propagation of Dalbergia sissoo Roxb. using Softwood and Hardwood Stem Cuttings. Journal of Arid Environments, 34, 235-245. https://doi.org/10.1006/jare.1996.0105
Mihaljevic, I., et al. (2013) In Vitro Sterilization Procedures for Micropropagation of ‘Oblacinska’sour Cherry. Journal of Agricultural Sciences, Belgrade, 58, 117-126. https://doi.org/10.2298/JAS1302117M
Falkiner, F. (1990) The Criteria for Choosing an Antibiotic for Control of Bacteria in Plant Tissue Culture.
Srivastava, N., et al. (2010) Standardization of Sterilization Protocol for Micropropagation of Aconitum heterophyllum-An Endangered Medicinal Herb. Academic Arena, 2, 37-42.
Al-Khayri, J.M. and Al-Bahrany, A.M. (2001) In Vitro Micropropagation of Citrus aurantifolia (lime). Current Science, 1242-1246.
Manickavasagam, M., et al. (2004) Agrobacterium-Mediated Genetic Transformation and Development of Herbicide-Resistant Sugarcane (Saccharum Species Hybrids) using Axillary Buds. Plant Cell Reports, 23, 134-143. https://doi.org/10.1007/s00299-004-0794-y
Kosir, P., Skof, S. and Luthar, Z. (2004) Direct Shoot Regeneration from Nodes of Phalaenopsis orchids. Acta Agriculturae Slovenica, 83, 233-242.
Ajithkumar, D. and Seeni, S. (1998) Rapid Clonal Multiplication through in Vitro Axillary Shoot Proliferation of Aegle marmelos (L.) Corr., a Medicinal Tree. Plant Cell Reports, 17, 422-426. https://doi.org/10.1007/s002990050418
Balaraju, K., Agastian, P. and Ignacimuthu, S. (2009) Micropropagation of Swertia chirata Buch.-Hams. ex Wall.: A Critically Endangered Medicinal Herb. Acta Physiologiae Plantarum, 31, 487-494. https://doi.org/10.1007/s11738-008-0257-0
Sen, M.K., et al. (2014) In Vitro Callus Induction and Plantlet Regeneration of Achyranthes aspera L., a High Value Medicinal Plant. Asian Pacific Journal of Tropical Biomedicine, 4, 40-46.