The shelf-life of a bioherbicide product is an important factor with regard to its commercial potential. The bioherbicidal efficacy of freshly fermented Myrothecium verrucaria (strain IMI 368023) (MV) mycelia formulations and MV mycelia preparations that had been freeze-dried and then stored at -20°C for 8 years was compared. Two concentrations of each formulation (1.0x and 0.5x) were tested, utilizing bioassays on seedlings of the weed, hemp sesbania ( Sesbania exaltata ) under greenhouse conditions or in darkness utilizing hydroponically grown seedlings. Freeze drying of freshly prepared MV mycelium produced a light, brownish-colored powder. Efficacy tests of this reconstituted 8-year-old dried material showed that some bioherbicidal activity was lost during long-term storage, i.e ., ~20% and ~60% seedling dry weight reduction at the 1.0x and 0.5x rate, respectively. Although plant mortality was greater in the fresh mycelial preparations treatments versus the freeze-dried and stored samples at all time points in the time-course, the stored material still caused >80% mortality, 15 days after treatment. Comparative disease progression ratings also showed a similar trend. Overall results show that freeze-drying MV is a useful method to reduce the bulk and cumbersomeness of storing heavy liquid fermentation product, while retaining bioherbicidal activity. These findings increase the utility of this bioherbicide and offer the potential to use the dried material in soil treatments or in a more concentrated form than attainable via the fermented product.
Wilmoth, J.R. (2015) World Population Projected to Reach 9.7 Billion by 2050. United Nations Website. http://www.un.org/en/development/desa/news/population/2015-report.html
Sharma, R. (2015) Allied Market Research. http://www.alliedmarketresearch.com
Heap, I. (2017) The International Survey of Herbicide Resistant Weeds. www.weedscience.org
Charudattan, R. and Walker, H.L. (1982) Biological Control of Weeds with Plant Pathogens. Wiley, New York.
Hoagland, R.E., Ed. (1990) Microbes and Microbial Products as Herbicides. American Chemical Society Symposium Series No. 439. ACS Books, Washington DC.
TeBeest, D.O. (1991) Microbial Control of Weeds. Chapman and Hall, New York. https://doi.org/10.1007/978-1-4615-9680-6
TeBeest, D.O. and Templeton, G.E. (1985) Mycoherbicides: Progress in the Biological Control of Weeds. Plant Disease, 69, 6-10.
Charudattan, R. (2001) Biological Control of Weeds by Means of Plant Pathogens: Significance for Integrated Weed Management in Modern Agro-Ecology. BioControl, 46, 229-260. https://doi.org/10.1023/A:1011477531101
Hoagland, R.E. (2001) Microbial Allelochemicals and Pathogens as Bioherbicidal Agents. Weed Technology, 15, 835-857. https://doi.org/10.1614/0890-037X(2001)015[0835:MAAPAB]2.0.CO;2
Charudattan, R. (2005) Ecological, Practical, and Political Inputs into Selection of Weed Targets: What Makes a Good Biological Control Target? Biological Control, 35, 183-196. https://doi.org/10.1016/j.biocontrol.2005.07.009
Boyetchko, S.M. and Peng, G. (2004) Challenges and Strategies for Development of Mycoherbicides. In: Arora, D.K., Ed., Fungal Biotechnology in Agricultural, Food, and Environmental Applications, Marcel Dekker, New York, 111-121.
Ghorbani, R., Leifert, C. and Seel, W. (2005) Biological Control of Weeds with Antagonistic Plant Pathogens. Advances in Agronomy, 86, 191-225. https://doi.org/10.1016/S0065-2113(05)86004-3
Hallett, S.G. (2005) Where Are the Bioherbicides? Weed Science, 53, 404-415. https://doi.org/10.1614/WS-04-157R2
Weaver, M.A., Lyn, M.E., Boyette, C.D. and Hoagland, R.E. (2007) Bioherbicides for Weed Control. In: Upadhyaya, M.K. and Blackshaw, R.E., Eds., Non-Chemical Weed Management, CAB International, New York, 93-110. https://doi.org/10.1079/9781845932909.0093
Bailey, K.L. (2014) The Bioherbicide Approach to Weed Control Using Plant Pathogens. In: Abrol, D.P., Ed., Integrated Pest Management: Current Concepts and Ecological Perspectives, Elsevier, San Diego, 245-266. https://doi.org/10.1016/B978-0-12-398529-3.00014-2
Duke, S.O, Scheffler, B.E., Boyette, C.D. and Dayan, F.E. (2015) Biotechnology in Weed Control. In: Kirk-Othmer Encyclopedia of Chemical Technology, John Wiley & Sons, New York, 1-25. https://doi.org/10.1002/0471238961.herbduke.a01.pub2
Cordeau, S., Triolet, M., Waymon, S., Steinberg, C. and Guillemin, J.P. (2016) Bioherbicides: Dead in the Water? Crop Protection, 87, 44-49. https://doi.org/10.1016/j.cropro.2016.04.016
Hoagland, R.E. and Boyette, C.D. (2016) Controlling Herbicide-Susceptible, -Tolerant and -Resistant Weeds with Microbial Bioherbicides. Outlooks on Pest Management, 27, 256-266. https://doi.org/10.1564/v27_dec_04
Bakerspigel, A. (1953) Soils as a Storage Medium for Fungi. Mycologia, 45, 596-604.
Tuite, J. (1969) Plant Pathological Methods: Fungi and Bacteria. Burgess Publication Co., Minneapolis, MN.
Amsellem, Z., Zidack, N.K., Quimby Jr., P. and Gressel, J. (1999) Long-Term Dry Preservation of Viable Mycelia of Two Mycoherbicidal Organisms. Crop Protection, 18, 643-649. https://doi.org/10.1016/S0261-2194(99)00070-8
Zidack, N. and Quimby Jr., P. (2002) Formulation of Bacteria for Biological Weed Control Using the Stabileze Method. Biocontrol Science and Technology, 12, 67-74. https://doi.org/10.1080/09583150120093112
Silman, R.W., Bothast, R.J. and Schisler, D.A. (1993) Production of Colletotrichum truncatum for Use as a Mycoherbicide: Effects of Culture, Drying and Storage on Recovery and Efficacy. Biotechnology Advances, 11, 561-575. https://doi.org/10.1016/0734-9750(93)90025-I
Connick, W.J., Daigle, D.J, Boyette, C.D., Williams, K.S., Vinyard, B.T. and Quimby Jr., P.C. (1996) Water Activity and Other Factors that Affect the Viability of Colletotrichum truncatum Conidia in Wheat Flour-Kaolin Granules (“Pesta”). Biocontrol Science and Technology, 6, 277-284. https://doi.org/10.1080/09583159650039467
Boyette, C.D., Abbas, H.K., Johnson, B.J., Hoagland, R.E. and Weaver, M.A. (2014) Biological Control of the Weed Sesbania exaltata Using a Microsclerotia Formulation of the Bioherbicide Colletotrichum truncatum. American Journal of Plant Sciences, 5, 2672-2685. https://doi.org/10.4236/ajps.2014.518282
Connick Jr., W.J., Jackson, M.A., Williams, K.S. and Boyette, C.D. (1997) Stability of Microsclerotial Inoculum of Colletotrichum truncatum Encapsulated in Wheat Flour-Kaolin Granules. World Journal of Microbiology and Biotechnology, 13, 549-554. https://doi.org/10.1023/A:1018517409756
Müller-Stover, D., Thomas, H., Sauerborn, J. and Kroschel, J. (2004) Two Granular Formulations of Fusarium oxysporum f. sp. orthoceras to Mitigate Sunflower Broomrape Orobanche cumana. BioControl, 49, 595-602. https://doi.org/10.1023/B:BICO.0000036438.66150.21
Shabana, Y., Singh, D., Ortiz-Ribbing, L.M. and Hallett, S.G. (2010) Production and Formulation of High Quality Conidia of Microsphaeropsis amaranthi for the Biological Control of Weedy Amaranthus Species. Biological Control, 55, 49-57. https://doi.org/10.1016/j.biocontrol.2010.06.014
Teshler, M.P., Ash, G.J., Zolotarov, Y. and Watson, A.K. (2007) Increased Shelf Life of a Bioherbicide through Combining Modified Atmosphere Packaging and Low Temperatures. Biocontrol Science and Technology, 17, 387-400. https://doi.org/10.1080/09583150701213695
Ash, G.J. (2010) The Science, Art and Business of Successful Bioherbicides. Biological Control, 52, 230-240. https://doi.org/10.1016/j.biocontrol.2009.08.007
Vogelgsang, S., Watson, A.K. and DiTommaso, A. (1998) Effect of Moisture, Inoculum Production, and Planting Substrate on Disease Reaction of Field Bindweed (Convolvulus arvensis L.) to the Fungal Pathogen, Phomopsis convolvulus. European Journal of Plant Pathology, 104, 253-262. https://doi.org/10.1023/A:1008681900370
Schnick, P.J., Stewart-Wade, S.M. and Boland, G.J. (2002) 2,4-D and Sclerotinia minor to Control Common Dandelion. Weed Science, 50, 173-178. https://doi.org/10.1614/0043-1745(2002)050[0173:DASMTC]2.0.CO;2
Bourdot, G.W., Baird, D., Hurrell, G.A. and De Jong, M.D. (2006) Safety Zones for a Sclerotinia sclerotiorum-Based Mycoherbicide: Accounting for Regional and Yearly Variation in Climate. Biocontrol Science and Technology, 16, 345-358. https://doi.org/10.1080/09583150500531966
Hoagland, R.E., Boyette, C.D. and Abbas, H.K. (2007) Myrothecium verrucaria Isolates and Formulations as Bioherbicide Agents for Kudzu. Biocontrol Science and Technology, 17, 721-731. https://doi.org/10.1080/09583150701527268
Walker, H.L. and Connick Jr., W.J. (1983) Sodium Alginate for Production and Formulation of Mycoherbicides. Weed Science, 31, 333-338.
Kennedy, A.C. (2017) Pseudomonas Species Having Weed-Suppressive Activity and Benign Soil Survival Traits for Annual Grass Weed Management. US Patent 9578884 B2.
Lee, H.B., Kim, J.-C., Hong, K.-S. and Kim, C.-J. (2008) Evaluation of a Fungal Strain of Myrothecium roridum F0252, as a Bioherbicide Agent. Plant Pathology Journal, 24, 453-460. https://doi.org/10.5423/PPJ.2008.24.4.453
Morin, L., Gianotti, A.F., Barker, R.D. and Johnston, P.R. (1998) Fusarium tumidum to Infect and Cause Severe Disease on Gorse (Ulex europaeus) in a Controlled Environment. Biocontrol Science and Technology, 8, 301-311. https://doi.org/10.1080/09583159830360
Abi, A.A. and Borromeo, C. (2008) Production and Efficacy of Exserohilum longirostratum as a Bioherbicide for the Control of Itchgrass (Rottboellia cochinchinensis) in Sugarcane Farms. Master’s Thesis, University of Putra, Malaysia.
Walker, H.L. and Tilley, A.M. (1997) Evaluation of an Isolate of Myrothecium verrucaria from Sicklepod (Senna obtusifolia) as a Potential Mycoherbicide Agent. Biological Control, 10, 104-112. https://doi.org/10.1006/bcon.1997.0559
Anderson, K.I. and Hallett, S.G. (2004) Herbicidal Spectrum and Activity of Myrothecium verrucaria. Weed Science, 52, 623-627. https://doi.org/10.1614/WS-03-101R1
Hoagland, R.E., Weaver, M.A. and Boyette, C.D. (2007). Myrothecium verrucaria: Bioherbicide, and Strategies to Reduce Its Non-target Risks. Allelopathy Journal, 19, 179-192.
Boyette, C.D., Walker, H.L. and Abbas, H.K. (2002) Biological Control of Kudzu (Pueraria lobata) with an Isolate of Myrothecium verrucaria. Biocontrol Science and Technology, 12, 75-82. https://doi.org/10.1080/09583150120093031
Boyette, C.D. and Hoagland, R.E. (2007) Evaluation of the Bioherbicide Myrothecium verrucaria for Weed Control in Tomato (Lycopersicon esculentum). Biocontrol Science and Technology, 17, 171-178. https://doi.org/10.1080/09583150600937451
Hoagland, R.E., McCallister, T.S., Boyette, C.D., Weaver, M.A. and Beecham, R.V. (2011) Effects of Myrothecium verrucaria on Morning-Glory (Ipomoea) Species. Allelopathy Journal, 27, 151-162.
Boyette, C.D., Hoagland, R.E. and Stetina, K.C. (2014) Biological Control of the Weed Hemp Sesbania (Sesbania exaltata) in Rice (Oryza sativa) by the Fungus Myrothecium verrucaria. Agronomy, 4, 74-89. https://doi.org/10.3390/agronomy4010074
Hoagland, R.E., Teaster, N.D. and Boyette, C.D. (2013) Bioherbicidal Effects of Myrothecium verrucaria on Glyphosate-Resistant and -Susceptible Palmer Amaranth Biotypes. Allelopathy Journal, 31, 367-376.
Boyette, C.D., Reddy, K.N. and Hoagland, R.E. (2006) Glyphosate and Bioherbicide Interaction for Controlling Kudzu (Pueraria lobata), Redvine (Brunnichia ovata), and Trumpetcreeper (Campsis radicans). Biocontrol Science and Technology, 16, 1067-1077. https://doi.org/10.1080/09583150600828742
Boyette, C.D., Hoagland, R.E., Weaver, M.A. and Reddy, K.N. (2008) Redvine (Brunnichia ovata) and Trumpetcreeper (Campsis radicans) Controlled under Field Conditions by a Synergistic Interaction of the Bioherbicide, Myrothecium verrucaria, with Glyphosate. Weed Biology and Management, 8, 39-45. https://doi.org/10.1111/j.1445-6664.2007.00272.x
Boyette, C.D., Hoagland, R.E., Weaver, M.A. and Stetina, K.C. (2014) Interaction of the Bioherbicide Myrothecium verrucaria and Glyphosate for Kudzu Control. American Journal of Plant Sciences, 5, 3943-3956. https://doi.org/10.4236/ajps.2014.526413
Abbas, H.K., Johnson, B.J., Shier, W.T., Tak, H., Jarvis, B.B. and Boyette, C.D. (2002) Phytotoxicity and Mammalian Cytotoxicity of Macrocyclic Trichothecenes from Myrothecium verrucaria. Phytochemistry, 59, 309-313. https://doi.org/10.1016/S0031-9422(01)00464-2
Boyette, C.D., Weaver, M.A., Hoagland, R.E. and Stetina, K.C. (2008) Submerged Culture of a Mycelial Formulation of a Bioherbicidal Strain of Myrothecium verrucaria with Mitigated Mycotoxin Production. World Journal of Microbiology and Biotechnology, 24, 2721-2726. https://doi.org/10.1007/s11274-008-9759-6
Walker, H.L. and Riley, J.A. (1982) Evaluation of Alternaria cassiae for the Biocontrol of Sicklepod (Cassia obtusifolia). Weed Science, 30, 651-654.
Horsfall, J.G. and Barratt, R.W. (1945) An Improved Grading System for Measuring Diseases. Phytopathology, 35, 655.
Hoagland, R.E. (1995) Hydroponic Seedling Bioassay for the Bioherbicides Colletotrichum truncatum and Alternaria cassia. Biocontrol Science and Technology, 5, 251-259. https://doi.org/10.1080/09583159550039710
Aguilera, J.M. and Karel, M. (1997) Preservation of Biological Materials under Desiccation. Critical Reviews in Food Science and Nutrition, 37, 287-309. https://doi.org/10.1080/10408399709527776